Cellosaurus cell line ZFL (CVCL

Cellosaurus ZFL (CVCL_3276)

Cross-references
Cell line name	ZFL
Synonyms	ZF-L; ZebraFish Liver; ZLE; Zebrafish Liver Epithelium
Accession	CVCL_3276
Resource Identification Initiative	To cite this cell line use: ZFL (RRID:CVCL_3276)
Comments	Group: Fish cell line. Doubling time: 2.54 +- 0.28 days (Note=In L-15-FBS), 6.6 +- 0.43 days (Note=In UltraMEM-ITES) (PubMed=28587087); 72 hours (ATCC=CRL-2643). Donor information: According to PubMed=7994634 obtained from pooling the livers of three fish, while ATCC mentions 10 individuals (ATCC=CRL-2643; PubMed=7994634). Derived from site: In situ; Liver; UBERON=UBERON_0002107. Cell type: Epithelial cell; CL=CL_0000066.
Species of origin	Danio rerio (Zebrafish) (Brachydanio rerio) (NCBI Taxonomy: 7955)
Sex of cell	Sex unspecified
Age at sampling	Adult
Category	Spontaneously immortalized cell line
Publications	DOI=10.1007/BF01404818 Bradford C.S., Sun L., Collodi P., Barnes D.W. Cell cultures from zebrafish embryos and adult tissues. J. Tissue Cult. Methods 16:99-107(1994) PubMed=7994634; DOI=10.1007/BF00757560 Ghosh C., Zhou Y.L., Collodi P. Derivation and characterization of a zebrafish liver cell line. Cell Biol. Toxicol. 10:167-176(1994) PubMed=24355757; DOI=10.1016/j.aquatox.2013.11.023 Eide M., Rusten M., Male R., Jensen K.H.M., Goksoyr A. A characterization of the ZFL cell line and primary hepatocytes as in vitro liver cell models for the zebrafish (Danio rerio). Aquat. Toxicol. 147:7-17(2014) PubMed=25536407; DOI=10.1016/j.cbpb.2014.12.002 Ruyra A., Torrealba D., Morera D., Tort L., MacKenzie S., Roher N. Zebrafish liver (ZFL) cells are able to mount an anti-viral response after stimulation with poly (I:C). Comp. Biochem. Physiol. 182B:55-63(2015) PubMed=28587087; DOI=10.3390/md15060147; PMCID=PMC5484097 Liu C.-L., Watson A.M., Place A.R., Jagus R. Taurine biosynthesis in a fish liver cell line (ZFL) adapted to a serum-free medium. Mar. Drugs 15:147.1-147.15(2017)
Cell line collections (Providers)	ATCC; CRL-2643 BCRC; 60454 BCRJ; 0256
Cell line databases/resources	CLO; CLO_0009725 FCS-free; 154-27-282-1-4-3
Encyclopedic resources	Wikidata; Q54995906
Entry history
Entry creation	04-Apr-2012
Last entry update	30-Jan-2024
Version number	21