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Cellosaurus OSC-20 (CVCL_3087)

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Cell line name OSC-20
Synonyms OSC20
Accession CVCL_3087
Resource Identification Initiative To cite this cell line use: OSC-20 (RRID:CVCL_3087)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: Japanese.
Doubling time: 27.4 hours (Note=At 35th passage) (DOI=10.15114/tr.25.93); 65 hours (Note=Lot 02032003), ~49 hours (Note=Lot 11152011), ~35 hours (Note=Lot 07182019) (JCRB=JCRB0197).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; CRISPR screening.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Variations; SNP array analysis.
Derived from site: Metastatic; Cervical lymph node; UBERON=UBERON_0002429.
Sequence variations
  • Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Pro81Leu (c.242C>T); ClinVar=VCV000833629; Zygosity=Homozygous (Cosmic-CLP=1240196; DepMap=ACH-001626).
  • Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.His1048Arg (c.3143A>G); ClinVar=VCV000450316; Zygosity=Heterozygous (Cosmic-CLP=1240196; DepMap=ACH-001626).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg249Ser (c.747G>T); ClinVar=VCV000012352; Zygosity=Homozygous (Cosmic-CLP=1240196; DepMap=ACH-001626).
HLA typing Source: PubMed=9119748
Class I
HLA-AA*02,11
HLA-BB*46,55
HLA-CC*w01,w09
Genome ancestry Source: PubMed=30894373

Origin% genome
African3.14
Native American0.23
East Asian, North75.72
East Asian, South16.66
South Asian0
European, North0
European, South4.24
Disease Tongue squamous cell carcinoma (NCIt: C4648)
Squamous cell carcinoma of the oral tongue (ORDO: Orphanet_457252)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Originate from same individual CVCL_WW78 ! TcOSC-20
Sex of cell Female
Age at sampling 59Y
Category Cancer cell line
STR profile Source(s): Cosmic-CLP=1240196; DepMap=ACH-001626

Markers:
AmelogeninX
CSF1PO12,13
D3S135816
D5S81813 (Cosmic-CLP=1240196)
13,14 (DepMap=ACH-001626)
D7S82010
D8S117912,14
D13S3178
D16S5399,13
D18S5113
D21S1129,32.2
FGA22
Penta D9,12
Penta E14,16
TH019,9.3
TPOX8,11
vWA18

Run an STR similarity search on this cell line
Publications

DOI=10.15114/tr.25.93
Toshikazu Yokoi, Shoji Hirata, Fumikatu Nishimura, Akira Miyakawa, Tetsuyo Odajima, Gen-iku Kohama, Yohichi Mochizuki;
Some properties of a newly established human cell line from a oral squamous carcinoma.
Tumor Res. 25:93-103(1990)

PubMed=9119748; DOI=10.1111/j.1349-7006.1997.tb00365.x; PMCID=PMC5921363
Akihiro Miyazaki, Noriyuki Sato, Shuji Takahashi, Aya Sasaki, Gen-iku Kohama, Akira Yamaguchi, Atsuhito Yagihashi, Kokichi Kikuchi;
Cytotoxicity of histocompatibility leukocyte antigen-DR8-restricted CD4 killer T cells against human autologous squamous cell carcinoma.
Jpn. J. Cancer Res. 88:191-197(1997)

DOI=10.15114/tr.35.25
Noriyuki Yonekura, Noriko Yokosawa, Yasushi Hariya, Hironari Dehari, Gen-iku Kohama, Nobuhiro Fujii;
Interferon-alpha and interferon-gamma augment apoptosis in oral carcinoma cells treated with Shiga toxin 2 (Stx2).
Tumor Res. 35:25-33(2000)

PubMed=12716470; DOI=10.1111/j.1349-7006.2002.tb01338.x; PMCID=PMC5927106
Hiroaki Kondo, Hiroeki Sahara, Akihiro Miyazaki, Yuki Nabeta, Yoshihiko Hirohashi, Takayuki Kanaseki, Akira Yamaguchi, Naoyuki Yamada, Kazuo Hirayama, Manabu Suzuki, Junji Hamuro ...Show all 16 authors... , Toshihiko Torigoe, Nobuaki Takahashi, Gen-iku Kohama, Hideyuki Ikeda, Noriyuki Sato; Show fewer authors
Natural antigenic peptides from squamous cell carcinoma recognized by autologous HLA-DR8-restricted CD4+ T cells.
Jpn. J. Cancer Res. 93:917-924(2002)

PubMed=19123955; DOI=10.1186/1479-5876-7-1; PMCID=PMC2627828
Jun-ichi Kobayashi, Toshihiko Torigoe, Yoshihiko Hirohashi, Satomi Idenoue, Akihiro Miyazaki, Akira Yamaguchi, Hiroyoshi Hiratsuka, Noriyuki Sato;
Comparative study on the immunogenicity between an HLA-A24-restricted cytotoxic T-cell epitope derived from survivin and that from its splice variant survivin-2B in oral cancer patients.
J. Transl. Med. 7:1.1-1.11(2009)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Fiona M. Behan, Francesco Iorio, Gabriele Picco, Emanuel Goncalves, Charlotte M. Beaver, Giorgia Migliardi, Rita Santos, Yanhua Rao, Francesco Sassi, Marika Pinnelli, Rizwan Ansari ...Show all 25 authors... , Sarah Harper, David Adam Jackson, Rebecca McRae, Rachel Pooley, Piers Wilkinson, Dieudonne J. van der Meer, David Dow, Carolyn A. Buser-Doepner, Andrea Bertotti, Livio Trusolino, Euan Alexander Stronach, Julio Saez-Rodriguez, Kosuke Yusa, Mathew J. Garnett; Show fewer authors
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) JCRB; FDSC0051 - Discontinued
JCRB; JCRB0197
Cell line databases/resources CLO; CLO_0009950
cancercelllines; CVCL_3087
Cell_Model_Passport; SIDM00565
Cosmic-CLP; 1240196
DepMap; ACH-001626
LINCS_LDP; LCL-1220
CRISP screens repositories BioGRID_ORCS_Cell_line; 979
Chemistry resources GDSC; 1240196
PharmacoDB; OSC20_1206_2019
Encyclopedic resources Wikidata; Q54936583
Gene expression databases ArrayExpress; E-MTAB-3610
GEO; GSM827220
GEO; GSM1670309
Polymorphism and mutation databases Cosmic; 1120702
Cosmic; 1995604
Progenetix; CVCL_3087
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number36