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Cellosaurus LLC-MK2 (CVCL_3009)

[Text version]
Cell line name LLC-MK2
Synonyms Llc-Mk2; LLC-MK-2; LLC-MK2 Original; LLCMK2; LLcMK2; Lilly Laboratories Culture-Monkey Kidney 2; MK2
Accession CVCL_3009
Resource Identification Initiative To cite this cell line use: LLC-MK2 (RRID:CVCL_3009)
Comments Group: Non-human primate cell line.
Group: Patented cell line.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CL-7.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CL-7.1.
Characteristics: Derived from the trypsinization of a pool of kidneys from 6 adult rhesus monkeys.
Virology: Susceptible to infection by SARS coronavirus (SARS-CoV) (PubMed=16494729).
Virology: Susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Species of origin Macaca mulatta (Rhesus macaque) (NCBI Taxonomy: 9544)
Hierarchy Children:
CVCL_U988 (NCTC 3196)CVCL_3069 (NCTC 3526)
Sex of cell Sex unspecified
Age at sampling Adult
Category Spontaneously immortalized cell line
Web pages https://en.vircell.com/products/llc-mk2-cell-line/

PubMed=13821019; DOI=10.1093/oxfordjournals.aje.a120078
Evans V.J., Kerr H.A., McQuilkin W.T., Earle W.R., Hull R.N.
Growth in vitro of a long-term strain of monkey-kidney cells in medium NCTC 109 free of any added protein.
Am. J. Hyg. 70:297-302(1959)

Cheery W.R., Hull R.N.
Studies and observations on the growth of mammalian cells in agitated fluid medium.
J. Biochem. Microbiol. Technol. Eng. 2:267-285(1960)

PubMed=14473108; DOI=10.4049/jimmunol.88.6.796
Meyer H.M. Jr., Hopps H.E., Rogers N.G., Brooks B.E., Bernheim B.C., Jones W.P., Nisalak A., Douglas R.D.
Studies on simian virus 40.
J. Immunol. 88:796-806(1962)

PubMed=14449901; DOI=10.1084/jem.115.5.903
Hull R.N., Cherry W.R., Tritch O.J.
Growth characteristics of monkey kidney cell strains LLC-MK1, LLC-MK2, and LLC-MK2(NCTC-3196) and their utility in virus research.
J. Exp. Med. 115:903-918(1962)

Hull R.N., Tritch O.J.
Characterization of cell strains by viral susceptibility.
Natl. Cancer Inst. Monogr. 7:161-172(1962)

PubMed=14185313; DOI=10.1126/science.146.3641.241
Cell Culture Collection Committee
Animal cell strains. The Cell Culture Collection Committee has assembled and certified 23 strains of animal cells.
Science 146:241-243(1964)

Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.
The animal cell culture collection.
In Vitro 5:1-16(1970)

PubMed=18605421; DOI=10.1093/jnci/45.5.951
Ikeuchi T., Sandberg A.A.
Chromosome pulverization in virus-induced heterokaryons of mammalian cells from different species.
J. Natl. Cancer Inst. 45:951-963(1970)

Petricciani J.C., Wallace R.E., McCoy D.W.
A comparison of three in vivo assays for cell tumorigenicity.
Cancer Res. 34:105-108(1974)

Hull R.N., Huseby R.M.
Enhanced production of plasminogen activator.
Patent number US3904480, 09-Sep-1975

PubMed=6298990; DOI=10.1016/0378-1135(82)90056-6
McPhee D.A., Parsonson I.M., Della-Porta A.J.
Comparative studies on the growth of Australian bluetongue virus serotypes in continuous cell lines and embryonated chicken eggs.
Vet. Microbiol. 7:401-410(1982)

PubMed=16494729; DOI=10.3201/eid1201.050496
Kaye M., Druce J., Tran T., Kostecki R., Chibo D., Morris J., Catton M., Birch C.
SARS-associated coronavirus replication in cell lines.
Emerg. Infect. Dis. 12:128-133(2006)

PubMed=28046048; DOI=10.1371/journal.pone.0169391
Dotti S., Lombardo T., Villa R., Cacciamali A., Zanotti C., Andreani N.A., Cinotti S., Ferrari M.
Transformation and tumorigenicity testing of simian cell lines and evaluation of poliovirus replication.
PLoS ONE 12:E0169391-E0169391(2017)

PubMed=33389257; DOI=10.1007/s10096-020-04106-0
Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)

Cell line collections (Providers) AddexBio; P0014004/4952
BCRC; 60092
BCRC; 60365
BCRJ; 0146
CLS; 305149
ECACC; 85062804
Cell line databases/resources CLO; CLO_0007330
CLDB; cl3214
CLDB; cl3215
CLDB; cl3218
CLDB; cl3219
CLDB; cl3220
Lonza; 128
Chemistry resources ChEMBL-Cells; CHEMBL3307684
ChEMBL-Targets; CHEMBL614691
PubChem_Cell_line; CVCL_3009
Encyclopedic resources Wikidata; Q54902701
Entry history
Entry creation04-Apr-2012
Last entry update29-Jun-2023
Version number33