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Cellosaurus LLC-MK2 (CVCL_3009)

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Cell line name LLC-MK2
Synonyms Llc-Mk2; LLC-MK-2; LLC-MK2 Original; LLCMK2; LLcMK2; Lilly Laboratories Culture-Monkey Kidney 2; MK2
Accession CVCL_3009
Resource Identification Initiative To cite this cell line use: LLC-MK2 (RRID:CVCL_3009)
Comments Group: Non-human primate cell line.
Group: Patented cell line.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CL-7.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CL-7.1.
Virology: Susceptible to infection by SARS coronavirus (SARS-CoV) (PubMed=16494729).
Virology: Susceptible to infection by SARS coronavirus 2 (SARS-CoV-2) (COVID-19) (PubMed=33389257).
Donor information: Derived from the trypsinization of a pool of kidneys from 6 adult rhesus monkeys.
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Species of origin Macaca mulatta (Rhesus macaque) (NCBI Taxonomy: 9544)
Hierarchy Children:
CVCL_U988 (NCTC 3196)CVCL_3069 (NCTC 3526)CVCL_E0XB (Ubigene LLC-MK2 APOE KO)
Sex of cell Sex unspecified
Age at sampling Adult
Category Spontaneously immortalized cell line
Web pages Info; Evident; -; https://evidentscientific.com/en/microscope-resource/image-gallery/confocal/epithelialindex
Provider; Vircell; -; https://www.vircell.com/en/products/llc-mk2-cell-line
Publications

PubMed=13821019; DOI=10.1093/oxfordjournals.aje.a120078
Virginia John Evans, Harold A. Kerr, William T. McQuilkin, Wilton Robinson Earle, Robert N. Hull;
Growth in vitro of a long-term strain of monkey-kidney cells in medium NCTC 109 free of any added protein.
Am. J. Hyg. 70:297-302(1959)

DOI=10.1002/jbmte.390020304
William R. Cherry, Robert N. Hull;
Studies and observations on the growth of mammalian cells in agitated fluid medium.
J. Biochem. Microbiol. Technol. Eng. 2:267-285(1960)

PubMed=14449901; DOI=10.1084/jem.115.5.903; PMCID=PMC2137539
Robert N. Hull, William R. Cherry, O. Jeannette Tritch;
Growth characteristics of monkey kidney cell strains LLC-MK1, LLC-MK2, and LLC-MK2(NCTC-3196) and their utility in virus research.
J. Exp. Med. 115:903-918(1962)

PubMed=14449902
Robert N. Hull, O. Jeannette Tritch;
Characterization of cell strains by viral susceptibility.
Natl. Cancer Inst. Monogr. 7:161-172(1962)

PubMed=14473108; DOI=10.4049/jimmunol.88.6.796
Harry Martin Meyer Jr., Hope Elizabeth Hopps, Nancy G. Rogers, Barbara E. Brooks, Barbara C. Bernheim, William P. Jones, Ananda Nisalak, Robert D. Douglas;
Studies on simian virus 40.
J. Immunol. 88:796-806(1962)

PubMed=14185313; DOI=10.1126/science.146.3641.241
Cell Culture Collection Committee
Animal cell strains. The Cell Culture Collection Committee has assembled and certified 23 strains of animal cells.
Science 146:241-243(1964)

DOI=10.1007/BF02618370
Cyril S. Stulberg, Lewis Lemon Coriell, Alexis John Kniazeff, John E. Shannon;
The animal cell culture collection.
In Vitro 5:1-16(1970)

PubMed=18605421; DOI=10.1093/jnci/45.5.951
Tatsuro Ikeuchi, Avery Aba Sandberg;
Chromosome pulverization in virus-induced heterokaryons of mammalian cells from different species.
J. Natl. Cancer Inst. 45:951-963(1970)

PubMed=4203458
John C. Petricciani, Roslyn Elizabeth Wallace, Donald W. McCoy;
A comparison of three in vivo assays for cell tumorigenicity.
Cancer Res. 34:105-108(1974)

Patent=US3904480
Robert N. Hull, Rolf M. Huseby;
Enhanced production of plasminogen activator.
Patent number US3904480, 09-Sep-1975

PubMed=6298990; DOI=10.1016/0378-1135(82)90056-6
Dale A. McPhee, Ian Malcolm Parsonson, Anthony James Della-Porta;
Comparative studies on the growth of Australian bluetongue virus serotypes in continuous cell lines and embryonated chicken eggs.
Vet. Microbiol. 7:401-410(1982)

PubMed=16494729; DOI=10.3201/eid1201.050496; PMCID=PMC3291385
Matthew Kaye, Julian Druce, Thomas Tran, Renata Kostecki, Doris Chibo, Jessica Morris, Mike Catton, Chris Birch;
SARS-associated coronavirus replication in cell lines.
Emerg. Infect. Dis. 12:128-133(2006)

PubMed=28046048; DOI=10.1371/journal.pone.0169391; PMCID=PMC5207746
Silvia Dotti, Tina Lombardo, Riccardo Villa, Andrea Cacciamali, Cinzia Zanotti, Nadia Andrea Andreani, Stefano Cinotti, Maura Ferrari;
Transformation and tumorigenicity testing of simian cell lines and evaluation of poliovirus replication.
PLoS ONE 12:e0169391.1-e0169391.13(2017)

PubMed=33389257; DOI=10.1007/s10096-020-04106-0; PMCID=PMC7778494
Nathalie Wurtz, Gwilherm Penant, Priscilla Jardot, Nathalie Duclos, Bernard La Scola;
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)

Cross-references
Cell line collections (Providers) AddexBio; P0014004/4952
ATCC; CCL-7
ATCC; CCL-7.1
BCRC; 60092
BCRC; 60365
BCRJ; 0146
CCTCC; GDC0156
CLS; 305149
ECACC; 85062804
IZSLER; BS CL 57
Cell line databases/resources CLO; CLO_0007330
CLDB; cl3214
CLDB; cl3215
CLDB; cl3218
CLDB; cl3219
CLDB; cl3220
CCRID; 1102MON-NIFDC00078
CCRID; 3101MONGNO6
CCRID; 4201MON-CCTCC00156
Lonza; 128
Chemistry resources ChEMBL-Cells; CHEMBL3307684
ChEMBL-Targets; CHEMBL614691
PubChem_Cell_line; CVCL_3009
Encyclopedic resources Wikidata; Q54902701
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number36