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Cellosaurus KP-3 (CVCL_3005)

[Text version]
Cell line name KP-3
Synonyms KP3
Accession CVCL_3005
Resource Identification Initiative To cite this cell line use: KP-3 (RRID:CVCL_3005)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: NCI RAS program mutant KRAS cell line panel.
Population: Japanese.
Doubling time: ~28 hours (Note=Lot 12012006), ~38 hours (Note=Lot 10112019) (JCRB=JCRB0178.0).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Metabolomics.
Omics: Phenotyping; CRISPR screening.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: Metastatic; Liver; UBERON=UBERON_0002107.
Sequence variations
  • Mutation; HGNC; HGNC:6407; KRAS; Simple; p.Gly12Val (c.35G>T); ClinVar=VCV000012583; Zygosity=Homozygous (PubMed=21607521; Cosmic-CLP=1298219; DepMap=ACH-000108).
  • Mutation; HGNC; HGNC:6770; SMAD4; Simple; p.Glu538Ter (c.1612G>T); ClinVar=VCV000581974; Zygosity=Homozygous (PubMed=18380791; Cosmic-CLP=1298219; DepMap=ACH-000108).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Pro153Alafs*28 (c.455dupC) (c.455_456insC) (p.P152fs) (c.459_460insC); ClinVar=VCV000182958; Zygosity=Heterozygous (Cosmic-CLP=1298219; DepMap=ACH-000108).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*24:02,24:02
HLA-BB*35:01,35:01
HLA-CC*08:01,08:01
Class II
HLA-DQDQA1*03:02,03:02
DQB1*03:02,03:02
HLA-DRDRB1*03:02,12:01
Genome ancestry Source: PubMed=30894373

Origin% genome
African0.33
Native American0.8
East Asian, North78.29
East Asian, South19.41
South Asian0.83
European, North0.07
European, South0.25
Disease Pancreatic adenosquamous carcinoma (NCIt: C5721)
Squamous cell carcinoma of pancreas (ORDO: Orphanet_424039)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_3006 (KP-3L)CVCL_0U13 (KP-3N)
Sex of cell Male
Age at sampling 75Y
Category Cancer cell line
STR profile Source(s): Cosmic-CLP=1298219; JCRB=JCRB0178.0; PubMed=25877200; Technion Genomics Center

Markers:
AmelogeninX
CSF1PO12
D1S165616,17.3
D2S44110,14
D2S133818,20
D3S135816
D5S81813
D7S82010,12
D8S117913,14
D10S124812
D12S39114
D13S3179,11
D16S5399,13
D18S5117
D19S43317
D21S1130,32
D22S104511
FGA23
Penta D12,13
Penta E15,17
TH016,9.3
TPOX9,11
vWA17,18 (Cosmic-CLP=1298219; JCRB=JCRB0178.0)
18 (PubMed=25877200; Technion Genomics Center)

Run an STR similarity search on this cell line
Publications

PubMed=2172194; DOI=10.1111/j.1349-7006.1990.tb03336.x; PMCID=PMC5917979
Yurika Ikeda, Mitsue Ezaki, Itsuro Hayashi, Daisuke Yasuda, Kazumichi Nakayama, Akira Kono;
Establishment and characterization of human pancreatic cancer cell lines in tissue culture and in nude mice.
Jpn. J. Cancer Res. 81:987-993(1990)

PubMed=21607521; DOI=10.3892/or.1.6.1223
Haruo Iguchi, Ryoji Morita, Daisuke Yasuda, Ryoichi Takayanagi, Yurika Ikeda, Yutaka Takada, Takao Shimazoe, Hajime Nawata, Akira Kono;
Alterations of the p53 tumor-suppressor gene and ki-ras oncogene in human pancreatic cancer-derived cell-lines with different metastatic potential.
Oncol. Rep. 1:1223-1227(1994)

PubMed=18380791; DOI=10.1111/j.1349-7006.2008.00779.x; PMCID=PMC11158928
Ayako Suzuki, Tatsuhiro Shibata, Yutaka Shimada, Yoshinori Murakami, Akira Horii, Keiko Shiratori, Setsuo Hirohashi, Johji Inazawa, Issei Imoto;
Identification of SMURF1 as a possible target for 7q21.3-22.1 amplification detected in a pancreatic cancer cell line by in-house array-based comparative genomic hybridization.
Cancer Sci. 99:986-994(2008)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Jordi Ginesta Barretina, Giordano Caponigro, Nicolas Stransky, Kavitha Venkatesan, Adam A. Margolin, Sungjoon Kim, Christopher J. Wilson, Joseph Lehar, Gregory V. Kryukov, Dmitriy Sonkin, Anupama Reddy ...Show all 61 authors... , Manway Liu, Lauren Murray, Michael F. Berger, John E. Monahan, Paula Morais, Jodi Meltzer, Adam Korejwa, Judit Jane-Valbuena, Felipa A. Mapa, Joseph Thibault, Eva Bric-Furlong, Pichai Raman, Aaron Shipway, Ingo H. Engels, Jill Cheng, Guo-Ying K. Yu, Jian-Jun Yu, Peter Aspesi Jr., Melanie de Silva, Kalpana Jagtap, Michael D. Jones, Li Wang, Charles Hatton, Emanuele Palescandolo, Supriya Gupta, Scott Mahan, Carrie Sougnez, Robert C. Onofrio, Ted Liefeld, Laura E. MacConaill, Wendy Winckler, Michael Reich, Nan-Xin Li, Jill P. Mesirov, Stacey B. Gabriel, Gad Getz, Kristin Ardlie, Vivien Chan, Vic E. Myer, Barbara L. Weber, Jeff Porter, Markus Warmuth, Peter Finan, Jennifer L. Harris, Matthew Langer Meyerson, Todd Robert Golub, Michael P. Morrissey, William Raj Sellers, Robert Schlegel, Levi Alexander Garraway; Show fewer authors
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=22585861; DOI=10.1158/2159-8290.CD-11-0224; PMCID=PMC5057396
Richard Marcotte, Kevin R. Brown, Fernando Jose Suarez Saiz, Azin Sayad, Konstantina Karamboulas, Paul M. Krzyzanowski, Fabrice Sircoulomb, Mauricio Medrano, Yaroslav Fedyshyn, Judice Lie-Yong Koh, Dewald van Dyk ...Show all 28 authors... , Bohdana Fedyshyn, Marianna Luhova, Glauber C. Brito, Franco J. Vizeacoumar, Frederick S. Vizeacoumar, Alessandro Datti, Dahlia Kasimer, Alla Buzina, Patricia Mero, Christine Misquitta, Josee Normand, Maliha Haider, Troy Ketela, Jeffrey L. Wrana, Robert Rottapel, Benjamin G. Neel, Jason Moffat; Show fewer authors
Essential gene profiles in breast, pancreatic, and ovarian cancer cells.
Cancer Discov. 2:172-189(2012)

DOI=10.4172/2324-9293.1000104
Markus Udo Wagenhauser, Felix Ruckert, Marco Niedergethmann, Robert Grutzmann, Hans-Detlev Saeger;
Distribution of characteristic mutations in native ductal adenocarcinoma of the pancreas and pancreatic cancer cell lines.
Cell Biol. Res. Ther. 2:1000104.1-1000104.5(2013)

PubMed=25485619; DOI=10.1038/nbt.3080
Christiaan Klijn, Steffen Durinck, Eric W. Stawiski, Peter M. Haverty, Zhao-Shi Jiang, Han-Bin Liu, Jeremiah Degenhardt, Oleg Mayba, Florian Gnad, Jin-Feng Liu, Gregoire Pau ...Show all 30 authors... , Jens Reeder, Yi Cao, Kiran Mukhyala, Suresh K. Selvaraj, Ma-Mie Yu, Gregory J. Zynda, Matthew J. Brauer, Thomas D. Wu, Robert Clifford Gentleman, Gerard Manning, Robert L. Yauch, Richard Bourgon, David Stokoe, Zora Modrusan, Richard M. Neve, Frederic J. de Sauvage, Jeffrey Settleman, Somasekar Seshagiri, Ze-Min Zhang; Show fewer authors
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26216984; DOI=10.1073/pnas.1501605112; PMCID=PMC4538616
Anneleen Daemen, David Peterson, Nisebita Sahu, Ron McCord, Xiang-Nan Du, Bonnie Liu, Katarzyna Kowanetz, Rebecca Hong, John Moffat, Min Gao, Aaron Boudreau ...Show all 22 authors... , Rana Mroue, Laura Corson, Thomas O'Brien, Jing Qing, Deepak Sampath, Mark Merchant, Robert L. Yauch, Gerard Manning, Jeffrey Settleman, Georgia Hatzivassiliou, Marie Evangelista; Show fewer authors
Metabolite profiling stratifies pancreatic ductal adenocarcinomas into subtypes with distinct sensitivities to metabolic inhibitors.
Proc. Natl. Acad. Sci. U.S.A. 112:E4410-E4417(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Jelle Scholtalbers, Sebastian Boegel, Thomas Bukur, Marius Byl, Sebastian Goerges, Patrick Sorn, Martin Loewer, Ugur Sahin, John C. Castle;
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Fiona M. Behan, Francesco Iorio, Gabriele Picco, Emanuel Goncalves, Charlotte M. Beaver, Giorgia Migliardi, Rita Santos, Yanhua Rao, Francesco Sassi, Marika Pinnelli, Rizwan Ansari ...Show all 25 authors... , Sarah Harper, David Adam Jackson, Rebecca McRae, Rachel Pooley, Piers Wilkinson, Dieudonne J. van der Meer, David Dow, Carolyn A. Buser-Doepner, Andrea Bertotti, Livio Trusolino, Euan Alexander Stronach, Julio Saez-Rodriguez, Kosuke Yusa, Mathew J. Garnett; Show fewer authors
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=32782605; DOI=10.3892/ol.2020.11825; PMCID=PMC7400074
Kohji Takagi, Johji Imura, Akiko Shimomura, Akira Noguchi, Takashi Minamisaka, Shinichi Tanaka, Takeshi Nishida, Hideki Hatta, Takahiko Nakajima;
Establishment of highly invasive pancreatic cancer cell lines and the expression of IL-32.
Oncol. Lett. 20:2888-2896(2020)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) JCRB; JCRB0178.0
JCRB; NIHS0275.0 - Discontinued
Cell line databases/resources CLO; CLO_0037108
cancercelllines; CVCL_3005
Cell_Model_Passport; SIDM00571
CGH-DB; 176-1
CGH-DB; 9371-4
Cosmic-CLP; 1298219
DepMap; ACH-000108
LINCS_LDP; LCL-1723
Anatomy/cell type resources BTO; BTO_0003551
Biological sample resources BioSample; SAMN03471644
BioSample; SAMN03472945
BioSample; SAMN10987829
CRISP screens repositories BioGRID_ORCS_Cell_line; 614
Chemistry resources GDSC; 1298219
PharmacoDB; KP3_784_2019
Encyclopedic resources Wikidata; Q54900364
Experimental variables resources EFO; EFO_0006622
Gene expression databases ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM827292
GEO; GSM887236
GEO; GSM888310
GEO; GSM1670003
Polymorphism and mutation databases Cosmic; 918053
IARC_TP53; 28373
LiGeA; CCLE_616
Progenetix; CVCL_3005
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000610
EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number41