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Cellosaurus HuH-1 (CVCL_2956)

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Cell line name HuH-1
Synonyms huH-1; HUH-1; huH 1; HuH1; HUh1; HUH1; huH1
Accession CVCL_2956
Resource Identification Initiative To cite this cell line use: HuH-1 (RRID:CVCL_2956)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: Liver Cancer Model Repository (LIMORE).
Part of: MD Anderson Cell Lines Project.
Part of: TCGA-110-CL cell line panel.
Population: Japanese.
Virology: Contains multiple copies of integrated hepatitis B virus (HBV) genomes.
Doubling time: 28.23 hours (PubMed=31378681); ~36 hours (Note=Lot 02172003), ~31 hours (Note=Lot 07192012), ~39 hours (Note=Lot 08102017), ~35 hours (Note=Lot 04022021) (JCRB=JCRB0199).
Microsatellite instability: Stable (MSS) (Sanger).
Transformant: NCBI_TaxID; 10407; Hepatitis B virus (HBV).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Genomics; Whole genome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Expression; Reverse-phase protein array.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; miRNA profiling; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Liver; UBERON=UBERON_0002107.
Sequence variations
  • Mutation; HGNC; HGNC:903; AXIN1; Simple; p.Asn302Profs*111 (c.904_907delAACC); Zygosity=Homozygous (PubMed=31395879; DepMap=ACH-000475).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg110Leu (c.329G>T); ClinVar=VCV000406597; Zygosity=Homozygous (PubMed=31395879; Cosmic-CLP=1298146; DepMap=ACH-000475).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*02:06,11:01
HLA-BB*08:20,35:08
HLA-CC*01:03,07:02
Class II
HLA-DRDRB1*04:05,13:03
Genome ancestry Source: PubMed=30894373

Origin% genome
African0.02
Native American0.87
East Asian, North80.09
East Asian, South18.1
South Asian0
European, North0
European, South0.92
Disease Adult hepatocellular carcinoma (NCIt: C7956)
Adult hepatocellular carcinoma (ORDO: Orphanet_210159)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_F0A3 (huSP)
Sex of cell Male
Age at sampling 53Y
Category Cancer cell line
STR profile Source(s): Cosmic-CLP=1298146; JCRB=JCRB0199; PubMed=25877200; PubMed=31378681

Markers:
AmelogeninX,Y
CSF1PO11
D3S135816,17
D5S81810,13
D7S82011
D8S117910,12
D13S31710,12
D16S5399
D18S5116
D21S1133.2
FGA24
Penta D12
Penta E16,18
TH016,9
TPOX8,11
vWA17,18

Run an STR similarity search on this cell line
Web pages Info; LCCL; -; https://lccl.zucmanlab.com/hcc/cellLines/Huh1
Info; MCLP; -; https://tcpaportal.org/mclp/
Publications

PubMed=6268479
Nam-Ho Huh, Tadashi Utakoji;
Production of HBs-antigen by two new human hepatoma cell lines and its enhancement by dexamethasone.
Gann 72:178-179(1981)

PubMed=6310497; DOI=10.1093/nar/11.16.5391; PMCID=PMC326285
Katsuro Koike, Midori Kobayashi, Hiroshi Mizusawa, Eisaku Yoshida, Katsuyuki Yaginuma, Masanori Taira;
Rearrangement of the surface antigen gene of hepatitis B virus integrated in the human hepatoma cell lines.
Nucleic Acids Res. 11:5391-5402(1983)

DOI=10.1007/978-4-431-68349-0_4
Jennifer J. Alexander;
Human hepatoma cell lines.
(In book chapter) Neoplasms of the liver; Okuda, Kunio & Ishak, Kamal George (eds.); pp.47-56; Springer; Tokyo; Japan (1987)

PubMed=8389256; DOI=10.1093/carcin/14.5.987
Ih-Chang Hsu, Takayoshi Tokiwa, William P. Bennett, Robert A. Metcalf, Judith A. Welsh, Tsung-Tang Sun, Curtis Craig Harris;
p53 gene mutation and integrated hepatitis B viral DNA sequences in human liver cancer cell lines.
Carcinogenesis 14:987-992(1993)

PubMed=8835345; DOI=10.1002/(SICI)1096-9071(199602)48:2<133::AID-JMV3>3.0.CO;2-A
So Tsuboi, Seishi Nagamori, Masahiro Miyazaki, Koichiro Mihara, Ken-ichi Fukaya, Kiichiro Teruya, Tsunenori Kosaka, Takao Tsuji, Masayoshi Namba;
Persistence of hepatitis C virus RNA in established human hepatocellular carcinoma cell lines.
J. Med. Virol. 48:133-140(1996)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Jordi Ginesta Barretina, Giordano Caponigro, Nicolas Stransky, Kavitha Venkatesan, Adam A. Margolin, Sungjoon Kim, Christopher J. Wilson, Joseph Lehar, Gregory V. Kryukov, Dmitriy Sonkin, Anupama Reddy ...Show all 61 authors... , Manway Liu, Lauren Murray, Michael F. Berger, John E. Monahan, Paula Morais, Jodi Meltzer, Adam Korejwa, Judit Jane-Valbuena, Felipa A. Mapa, Joseph Thibault, Eva Bric-Furlong, Pichai Raman, Aaron Shipway, Ingo H. Engels, Jill Cheng, Guo-Ying K. Yu, Jian-Jun Yu, Peter Aspesi Jr., Melanie de Silva, Kalpana Jagtap, Michael D. Jones, Li Wang, Charles Hatton, Emanuele Palescandolo, Supriya Gupta, Scott Mahan, Carrie Sougnez, Robert C. Onofrio, Ted Liefeld, Laura E. MacConaill, Wendy Winckler, Michael Reich, Nan-Xin Li, Jill P. Mesirov, Stacey B. Gabriel, Gad Getz, Kristin Ardlie, Vivien Chan, Vic E. Myer, Barbara L. Weber, Jeff Porter, Markus Warmuth, Peter Finan, Jennifer L. Harris, Matthew Langer Meyerson, Todd Robert Golub, Michael P. Morrissey, William Raj Sellers, Robert Schlegel, Levi Alexander Garraway; Show fewer authors
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=23505090; DOI=10.1002/hep.26402
Kai Wang, Ho Yeong Lim, Stephanie Shi, Jeeyun Lee, Shi-Bing Deng, Tao Xie, Zhou Zhu, Yu-Li Wang, David Pocalyko, Wei Jennifer Yang, Paul A. Rejto ...Show all 14 authors... , Mao Mao, Cheol-Keun Park, Jiang-Chun Xu; Show fewer authors
Genomic landscape of copy number aberrations enables the identification of oncogenic drivers in hepatocellular carcinoma.
Hepatology 58:706-717(2013)

PubMed=25485619; DOI=10.1038/nbt.3080
Christiaan Klijn, Steffen Durinck, Eric W. Stawiski, Peter M. Haverty, Zhao-Shi Jiang, Han-Bin Liu, Jeremiah Degenhardt, Oleg Mayba, Florian Gnad, Jin-Feng Liu, Gregoire Pau ...Show all 30 authors... , Jens Reeder, Yi Cao, Kiran Mukhyala, Suresh K. Selvaraj, Ma-Mie Yu, Gregory J. Zynda, Matthew J. Brauer, Thomas D. Wu, Robert Clifford Gentleman, Gerard Manning, Robert L. Yauch, Richard Bourgon, David Stokoe, Zora Modrusan, Richard M. Neve, Frederic J. de Sauvage, Jeffrey Settleman, Somasekar Seshagiri, Ze-Min Zhang; Show fewer authors
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Jelle Scholtalbers, Sebastian Boegel, Thomas Bukur, Marius Byl, Sebastian Goerges, Patrick Sorn, Martin Loewer, Ugur Sahin, John C. Castle;
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005; PMCID=PMC5501076
Jun Li, Wei Zhao, Rehan Akbani, Wen-Bin Liu, Zhen-Lin Ju, Shi-Yun Ling, Christopher P. Vellano, Paul Roebuck, Qing-Hua Yu, A. Karina Eterovic, Lauren Averett Byers ...Show all 25 authors... , Michael A. Davies, Wan-Leng Deng, Y.N. Vashisht Gopal, Guo Chen, Erika Maria von Euw, Dennis Joseph Slamon, Dylan Conklin, John Victor Heymach, Adi F. Gazdar, John D. Minna, Jeffrey N. Myers, Yi-Ling Lu, Gordon B. Mills, Han Liang; Show fewer authors
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

PubMed=29892436; DOI=10.1098/rsos.172472; PMCID=PMC5990783
Setsuko Shioda, Fumio Kasai, Ken Watanabe, Kohei Kawakami, Azusa Ohtani, Masashi Iemura, Midori Ozawa, Akemi Arakawa, Noriko Hirayama, Eiko Kawaguchi, Tomoko Tano ...Show all 15 authors... , Sayaka Miyata, Motonobu Satoh, Norio Shimizu, Arihiro Kohara; Show fewer authors
Screening for 15 pathogenic viruses in human cell lines registered at the JCRB Cell Bank: characterization of in vitro human cells by viral infection.
R. Soc. Open Sci. 5:172472-172472(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31063779; DOI=10.1053/j.gastro.2019.05.001
Stefano Caruso, Anna-Line Calatayud, Jill Pilet, Tiziana La Bella, Samia Rekik, Sandrine Imbeaud, Eric Letouze, Lea Meunier, Quentin Bayard, Nataliya Rohr-Udilova, Camille Peneau ...Show all 20 authors... , Bettina Grasl-Kraupp, Leanne de Koning, Berengere Ouine, Paulette Bioulac-Sage, Gabrielle Couchy, Julien Calderaro, Jean-Charles Nault, Jessica Zucman-Rossi, Sandra Rebouissou; Show fewer authors
Analysis of liver cancer cell lines identifies agents with likely efficacy against hepatocellular carcinoma and markers of response.
Gastroenterology 157:760-776(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31378681; DOI=10.1016/j.ccell.2019.07.001; PMCID=PMC7505724
Zhi-Xin Qiu, Hong Li, Zheng-Tao Zhang, Zhen-Feng Zhu, Sheng He, Xu-Jun Wang, Peng-Cheng Wang, Jian-Jie Qin, Li-Ping Zhuang, Wei Wang, Fu-Bo Xie ...Show all 33 authors... , Ying Gu, Ke-Ke Zou, Chao Li, Chun Li, Chen-Hua Wang, Jin Cen, Xiao-Tao Chen, Ya-Jing Shu, Zhao Zhang, Lu-Lu Sun, Li-Hua Min, Yong Fu, Xiao-Wu Huang, Hui Lv, He Zhou, Yuan Ji, Zhi-Gang Zhang, Zhi-Qiang Meng, Xiao-Lei Shi, Hai-Bin Zhang, Yi-Xue Li, Li-Jian Hui; Show fewer authors
A pharmacogenomic landscape in human liver cancers.
Cancer Cell 36:179-193.e11(2019)

PubMed=31395879; DOI=10.1038/s41467-019-11415-2; PMCID=PMC6687785
Katharine Yu, Bin Chen, Dvir Aran, Joe Charalel, Christina Yau, Douglas M. Wolf, Laura J. van 't Veer, Atul Janardhan Butte, Ted Goldstein, Marina Sirota;
Comprehensive transcriptomic analysis of cell lines as models of primary tumors across 22 tumor types.
Nat. Commun. 10:3574.1-3574.11(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023; PMCID=PMC7339254
David Paul Nusinow, John Szpyt, Mahmoud Ghandi, Christopher M. Rose, E. Robert McDonald 3rd, Marian Kalocsay, Judit Jane-Valbuena, Ellen T. Gelfand, Devin K. Schweppe, Mark P. Jedrychowski, Javad Golji ...Show all 20 authors... , Dale A. Porter, Tomas Rejtar, Y. Karen Wang, Gregory V. Kryukov, Frank Stegmeier, Brian K. Erickson, Levi Alexander Garraway, William Raj Sellers, Steven P. Gygi; Show fewer authors
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)

PubMed=32899426; DOI=10.3390/cancers12092510; PMCID=PMC7565451
Dominique Scherer, Marcela Davila-Lopez, Benjamin Goeppert, Sanna Abrahamsson, Rosa Gonzalez Silos, Igor Nova, Katherine Marcelain, Juan Carlos Roa, David Ibberson, Sinan U. Umu, Trine Ballestad Rounge ...Show all 13 authors... , Stephanie Roessler, Justo Lorenzo-Bermejo; Show fewer authors
RNA sequencing of hepatobiliary cancer cell lines: data and applications to mutational and transcriptomic profiling.
Cancers (Basel) 12:2510.1-2510.14(2020)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) JCRB; JCRB0199
Cell line databases/resources CLO; CLO_0009988
cancercelllines; CVCL_2956
Cell_Model_Passport; SIDM00586
CGH-DB; 452-3
Cosmic-CLP; 1298146
DepMap; ACH-000475
LIMORE; HuH1
LINCS_LDP; LCL-1940
Lonza; 1679
Anatomy/cell type resources BTO; BTO_0005773
Biological sample resources BioSample; SAMN03473212
BioSample; SAMN10988262
CRISP screens repositories BioGRID_ORCS_Cell_line; 644
Chemistry resources GDSC; 1298146
PharmacoDB; huH1_637_2019
Encyclopedic resources Wikidata; Q54896843
Experimental variables resources EFO; EFO_0006445
Gene expression databases ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM827219
GEO; GSM887146
GEO; GSM888218
GEO; GSM936761
GEO; GSM1374565
GEO; GSM1669918
GEO; GSM2551568
Polymorphism and mutation databases Cosmic; 1187328
Cosmic; 2023878
IARC_TP53; 30077
LiGeA; CCLE_117
Progenetix; CVCL_2956
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000610
EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number42