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Cellosaurus MT-4 (CVCL_2632)

[Text version]
Cell line name MT-4
Synonyms MT4
Accession CVCL_2632
Resource Identification Initiative To cite this cell line use: MT-4 (RRID:CVCL_2632)
Comments Problematic cell line: Partially contaminated. Two different lots formerly distributed by NIH-ARP; 13 P7 3/9/92 and 150048; were shown not to be authentic MT-4 (PubMed=9261343; PubMed=31554688).
Population: Japanese.
Characteristics: Was established by co-cultivation of cells from a 50 year old male T-cell leukemia patient with cord leukocytes from a male infant (CelloPub=CLPUB00542).
Virology: HTLV-1 producing cell line. Expresses Tax. Is permissive for replication of an HIV-1 gp41 mutant lacking the cytoplasmic tail.
Doubling time: ~30 hours (Note=Lot 102799) (JCRB=JCRB1216).
Transformant: NCBI_TaxID; 11908; Human T-lymphotropic virus 1 (HTLV-1).
Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168.
Cell type: T-cell; CL=CL_0000084.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_RW54 (MT4/HIV-1)CVCL_RW55 (MT4C5)
Sex of cell Male
Age at sampling <1D
Category Transformed cell line
STR profile Source(s): JCRB=JCRB0135; JCRB=JCRB1216; PubMed=31554688; PubMed=32938764

Markers:
AmelogeninX,Y
CSF1PO11,12
D3S135817
D5S81810,11
D7S8208,9.3,10 (JCRB=JCRB1216)
8,10 (JCRB=JCRB0135; PubMed=31554688; PubMed=32938764)
D8S117910,15
D13S31712
D16S5399,12
D18S5113
D21S1128,30.3
FGA23
Penta D10,13
Penta E5,15
TH017
TPOX11
vWA17,18

Run an STR similarity search on this cell line
Web pages Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/m/cell-lines-detail-117.html
Publications

CLPUB00542
Isao Miyoshi, Hirokuni Taguchi, Ichiro Kubonishi, Shizuo Yoshimoto, Yuji Ohtsuki, Yukimasa Shiraishi, Tadaatsu Akagi;
Type C virus-producing cell lines derived from adult T cell leukemia.
Gann Monogr. Cancer Res. 28:219-228(1982)

PubMed=2413616; DOI=10.1016/0042-6822(85)90010-8
Shinji Harada, Yoshio Koyanagi, Naoki Yamamoto;
Infection of human T-lymphotropic virus type-I (HTLV-I)-bearing MT-4 cells with HTLV-III (AIDS virus): chronological studies of early events.
Virology 146:272-281(1985)

PubMed=1765142; DOI=10.1016/0014-5793(91)81402-t
Satoshi Orita, Akihiko Saiga, Shin Takagi, Tatsuro Tanaka, Koichi Okumura, Yuko Aono, Yorio Hinuma, Hisanaga Igarashi;
A novel alternatively spliced viral mRNA transcribed in cells infected with human T cell leukemia virus type 1 is mainly responsible for expressing p21X protein.
FEBS Lett. 295:127-134(1991)

PubMed=1364232
Agnes Gyuris, Gabor Vajda, Istvan Foldes;
Establishment of an MT4 cell line persistently producing infective HIV-1 particles.
Acta Microbiol. Hung. 39:271-279(1992)

PubMed=8927062
Mikhail Alexandrovich Susloparov, A.V. Krendel'shchikov, Marina M. Bakhtina, A.I. Bedristov, Igor V. Babkin, Valery V. Gutorov, Alexey M. Eroshkin, Olga A. Mamaeva;
Cloning of proviral DNA sequences of the human T-cell leukemia virus type 1 (HTLV-1) from the genome of cultured MT-4 cells.
Mol. Gen. Mikrobiol. Virusol. 1996:32-39(1996)

PubMed=8957066; DOI=10.1111/j.1349-7006.1996.tb03112.x; PMCID=PMC5920998
Nobuhiro Kawasaki, Yoshinobu Matsuo, Tadashi Yoshino, Hiroyuki Yanai, Takashi Oka, Norihiro Teramoto, Cao Liu, Eisaku Kondo, Jun Minowada, Tadaatsu Akagi;
Metastatic potential of lymphoma/leukemia cell lines in SCID mice is closely related to expression of CD44.
Jpn. J. Cancer Res. 87:1070-1077(1996)

PubMed=9261343; DOI=10.1128/JVI.71.9.6277-6278.1997; PMCID=PMC191899
Kuan-Teh Jeang, David Daniel Derse, Martha Matocha, Opendra Sharma;
Expression status of Tax protein in human T-cell leukemia virus type 1-transformed MT4 cells: recall of MT4 cells distributed by the NIH AIDS Research and Reference Reagent Program.
J. Virol. 71:6277-6278(1997)

PubMed=29892436; DOI=10.1098/rsos.172472; PMCID=PMC5990783
Setsuko Shioda, Fumio Kasai, Ken Watanabe, Kohei Kawakami, Azusa Ohtani, Masashi Iemura, Midori Ozawa, Akemi Arakawa, Noriko Hirayama, Eiko Kawaguchi, Tomoko Tano ...Show all 15 authors... , Sayaka Miyata, Motonobu Satoh, Norio Shimizu, Arihiro Kohara; Show fewer authors
Screening for 15 pathogenic viruses in human cell lines registered at the JCRB Cell Bank: characterization of in vitro human cells by viral infection.
R. Soc. Open Sci. 5:172472-172472(2018)

PubMed=31554688; DOI=10.1128/JVI.01390-19; PMCID=PMC6880167
Melissa Victoria Fernandez, Krista A. Delviks-Frankenberry, David A. Scheiblin, Christine Happel, Vinay K. Pathak, Eric O. Freed;
Authentication analysis of MT-4 cells distributed by the National Institutes of Health AIDS Reagent Program.
J. Virol. 93:e01390.19.1-e01390.19.8(2019)

PubMed=32938764; DOI=10.1128/JVI.01334-20; PMCID=PMC7654282
Melissa Victoria Fernandez, Huxley K. Hoffman, Nairi Pezeshkian, Philip R. Tedbury, Schuyler B. van Engelenburg, Eric O. Freed;
Elucidating the basis for permissivity of the MT-4 T-cell line to replication of an HIV-1 mutant lacking the gp41 cytoplasmic tail.
J. Virol. 94:e01334.20.1-e01334.20.27(2020)

Cross-references
Cell line collections (Providers) BEI_Resources; ARP-120
ECACC; 08081402
JCRB; JCRB0135 - Discontinued
JCRB; JCRB1216 - Discontinued
JCRB; NIHS0145 - Discontinued
JCRB; NIHS0529 - Discontinued
KCB; KCB 200308YJ
Cell line databases/resources CLO; CLO_0007891
CLDB; cl3594
CLDB; cl3599
Anatomy/cell type resources BTO; BTO_0003030
Biological sample resources BioSample; SAMN03470803
BioSample; SAMN03472442
Chemistry resources ChEMBL-Cells; CHEMBL3308375
ChEMBL-Targets; CHEMBL388
PubChem_Cell_line; CVCL_2632
Encyclopedic resources Wikidata; Q54906940
Polymorphism and mutation databases Cosmic; 1191697
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number27