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Cellosaurus MT-4 (CVCL_2632)

[Text version]
Cell line name MT-4
Synonyms MT4
Accession CVCL_2632
Resource Identification Initiative To cite this cell line use: MT-4 (RRID:CVCL_2632)
Comments Problematic cell line: Partially contaminated. Two different lots formerly distributed by NIH-ARP; 13 P7 3/9/92 and 150048; were shown not to be authentic MT-4 (PubMed=9261343; PubMed=31554688).
Population: Japanese.
Characteristics: Was established by co-cultivation of cells from a 50 year old male T-cell leukemia patient with cord leukocytes from a male infant (CelloPub=CLPUB00542).
Virology: HTLV-1 producing cell line. Expresses Tax. Is permissive for replication of an HIV-1 gp41 mutant lacking the cytoplasmic tail.
Doubling time: ~30 hours (Note=Lot 102799) (JCRB=JCRB1216).
Transformant: NCBI_TaxID; 11908; Human T-lymphotropic virus 1 (HTLV-1).
Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168.
Cell type: T-cell; CL=CL_0000084.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
Sex of cell Male
Age at sampling <1D
Category Transformed cell line
STR profile Source(s): JCRB=JCRB0135; JCRB=JCRB1216; PubMed=31554688; PubMed=32938764

D7S8208,9.3,10 (JCRB=JCRB1216)
8,10 (JCRB=JCRB0135; PubMed=31554688; PubMed=32938764)
Penta D10,13
Penta E5,15

Run an STR similarity search on this cell line
Web pages https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/m/cell-lines-detail-117.html

Miyoshi I., Taguchi H., Kubonishi I., Yoshimoto S., Ohtsuki Y., Shiraishi Y., Akagi T.
Type C virus-producing cell lines derived from adult T cell leukemia.
Gann Monogr. Cancer Res. 28:219-228(1982)

PubMed=2413616; DOI=10.1016/0042-6822(85)90010-8
Harada S., Koyanagi Y., Yamamoto N.
Infection of human T-lymphotropic virus type-I (HTLV-I)-bearing MT-4 cells with HTLV-III (AIDS virus): chronological studies of early events.
Virology 146:272-281(1985)

PubMed=1765142; DOI=10.1016/0014-5793(91)81402-t
Orita S., Saiga A., Takagi S., Tanaka T., Okumura K., Aono Y., Hinuma Y., Igarashi H.
A novel alternatively spliced viral mRNA transcribed in cells infected with human T cell leukemia virus type 1 is mainly responsible for expressing p21X protein.
FEBS Lett. 295:127-134(1991)

Gyuris A., Vajda G., Foldes I.
Establishment of an MT4 cell line persistently producing infective HIV-1 particles.
Acta Microbiol. Hung. 39:271-279(1992)

Susloparov M.A., Krendel'shchikov A.V., Bakhtina M.M., Bedristov A.I., Babkin I.V., Gutorov V.V., Eroshkin A.M., Mamaeva O.A.
Cloning of proviral DNA sequences of the human T-cell leukemia virus type 1 (HTLV-1) from the genome of cultured MT-4 cells.
Mol. Genet. Mikrobiol. Virusol. 1996:32-39(1996)

PubMed=8957066; DOI=10.1111/j.1349-7006.1996.tb03112.x
Kawasaki N., Matsuo Y., Yoshino T., Yanai H., Oka T., Teramoto N., Liu C., Kondo E., Minowada J., Akagi T.
Metastatic potential of lymphoma/leukemia cell lines in SCID mice is closely related to expression of CD44.
Jpn. J. Cancer Res. 87:1070-1077(1996)

PubMed=9261343; DOI=10.1128/JVI.71.9.6277-6278.1997
Jeang K.-T., Derse D.D., Matocha M., Sharma O.
Expression status of Tax protein in human T-cell leukemia virus type 1-transformed MT4 cells: recall of MT4 cells distributed by the NIH AIDS Research and Reference Reagent Program.
J. Virol. 71:6277-6278(1997)

PubMed=29892436; DOI=10.1098/rsos.172472
Shioda S., Kasai F., Watanabe K., Kawakami K., Ohtani A., Iemura M., Ozawa M., Arakawa A., Hirayama N., Kawaguchi E., Tano T., Miyata S., Satoh M., Shimizu N., Kohara A.
Screening for 15 pathogenic viruses in human cell lines registered at the JCRB Cell Bank: characterization of in vitro human cells by viral infection.
R. Soc. Open Sci. 5:172472-172472(2018)

PubMed=31554688; DOI=10.1128/JVI.01390-19
Fernandez M.V., Delviks-Frankenberry K.A., Scheiblin D.A., Happel C., Pathak V.K., Freed E.O.
Authentication analysis of MT-4 cells distributed by the NIH AIDS Reagent Program.
J. Virol. 93:e01390.19.1-e01390.19.8(2019)

PubMed=32938764; DOI=10.1128/JVI.01334-20
Fernandez M.V., Hoffman H.K., Pezeshkian N., Tedbury P.R., van Engelenburg S.B., Freed E.O.
Elucidating the basis for permissivity of the MT-4 T-cell line to replication of an HIV-1 mutant lacking the gp41 cytoplasmic tail.
J. Virol. 94:e01334.20.1-e01334.20.27(2020)

Cell line collections (Providers) ECACC; 08081402
HIVReagentProgram; ARP-120
JCRB; JCRB0135 - Discontinued
JCRB; JCRB1216 - Discontinued
JCRB; NIHS0145 - Discontinued
JCRB; NIHS0529 - Discontinued
KCB; KCB 200308YJ
Cell line databases/resources CLO; CLO_0007891
CLDB; cl3594
CLDB; cl3599
Anatomy/cell type resources BTO; BTO:0003030
Biological sample resources BioSample; SAMN03470803
BioSample; SAMN03472442
Chemistry resources ChEMBL-Cells; CHEMBL3308375
ChEMBL-Targets; CHEMBL388
PubChem_Cell_line; CVCL_2632
Encyclopedic resources Wikidata; Q54906940
Polymorphism and mutation databases Cosmic; 1191697
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number24