ID MT-4 AC CVCL_2632 SY MT4 DR BTO; BTO_0003030 DR CLO; CLO_0007891 DR CLDB; cl3594 DR CLDB; cl3599 DR BEI_Resources; ARP-120 DR BioSample; SAMN03470803 DR BioSample; SAMN03472442 DR ChEMBL-Cells; CHEMBL3308375 DR ChEMBL-Targets; CHEMBL388 DR Cosmic; 1191697 DR ECACC; 08081402 DR JCRB; JCRB0135 DR JCRB; JCRB1216 DR JCRB; NIHS0145 DR JCRB; NIHS0529 DR KCB; KCB 200308YJ DR PubChem_Cell_line; CVCL_2632 DR Wikidata; Q54906940 RX CelloPub=CLPUB00542; RX PubMed=1364232; RX PubMed=1765142; RX PubMed=2413616; RX PubMed=8927062; RX PubMed=8957066; RX PubMed=9261343; RX PubMed=29892436; RX PubMed=31554688; RX PubMed=32938764; WW Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/m/cell-lines-detail-117.html CC Problematic cell line: Partially contaminated. Two different lots formerly distributed by NIH-ARP; 13 P7 3/9/92 and 150048; were shown not to be authentic MT-4 (PubMed=9261343; PubMed=31554688). CC Population: Japanese. CC Characteristics: Was established by co-cultivation of cells from a 50 year old male T-cell leukemia patient with cord leukocytes from a male infant (CelloPub=CLPUB00542). CC Virology: HTLV-1 producing cell line. Expresses Tax. Is permissive for replication of an HIV-1 gp41 mutant lacking the cytoplasmic tail. CC Doubling time: ~30 hours (Note=Lot 102799) (JCRB=JCRB1216). CC Transformant: NCBI_TaxID; 11908; Human T-lymphotropic virus 1 (HTLV-1). CC Discontinued: JCRB; JCRB0135; probable. CC Discontinued: JCRB; JCRB1216; probable. CC Discontinued: JCRB; NIHS0145; true. CC Discontinued: JCRB; NIHS0529; true. CC Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168. CC Cell type: T-cell; CL=CL_0000084. ST Source(s): JCRB=JCRB0135; JCRB=JCRB1216; PubMed=31554688; PubMed=32938764 ST Amelogenin: X,Y ST CSF1PO: 11,12 ST D13S317: 12 ST D16S539: 9,12 ST D18S51: 13 ST D21S11: 28,30.3 ST D3S1358: 17 ST D5S818: 10,11 ST D7S820: 8,9.3,10 (JCRB=JCRB1216) ST D7S820: 8,10 (JCRB=JCRB0135; PubMed=31554688; PubMed=32938764) ST D8S1179: 10,15 ST FGA: 23 ST Penta D: 10,13 ST Penta E: 5,15 ST TH01: 7 ST TPOX: 11 ST vWA: 17,18 OX NCBI_TaxID=9606; ! Homo sapiens (Human) SX Male AG <1D CA Transformed cell line DT Created: 04-04-12; Last updated: 10-04-25; Version: 27 CH CVCL_RW54 ! MT4/HIV-1 CH CVCL_RW55 ! MT4C5 // RX PubMed=1765142; DOI=10.1016/0014-5793(91)81402-t; RA Orita, Satoshi RA Saiga, Akihiko RA Takagi, Shin RA Tanaka, Tatsuro RA Okumura, Koichi RA Aono, Yuko RA Hinuma, Yorio RA Igarashi, Hisanaga RT "A novel alternatively spliced viral mRNA transcribed in cells RT infected with human T cell leukemia virus type 1 is mainly responsible RT for expressing p21X protein."; RL FEBS Lett. 295:127-134(1991). // RX PubMed=29892436; DOI=10.1098/rsos.172472; PMCID=PMC5990783; RA Shioda, Setsuko RA Kasai, Fumio RA Watanabe, Ken RA Kawakami, Kohei RA Ohtani, Azusa RA Iemura, Masashi RA Ozawa, Midori RA Arakawa, Akemi RA Hirayama, Noriko RA Kawaguchi, Eiko RA Tano, Tomoko RA Miyata, Sayaka RA Satoh, Motonobu RA Shimizu, Norio RA Kohara, Arihiro RT "Screening for 15 pathogenic viruses in human cell lines registered at RT the JCRB Cell Bank: characterization of in vitro human cells by viral RT infection."; RL R. Soc. Open Sci. 5:172472-172472(2018). // RX PubMed=2413616; DOI=10.1016/0042-6822(85)90010-8; RA Harada, Shinji RA Koyanagi, Yoshio RA Yamamoto, Naoki RT "Infection of human T-lymphotropic virus type-I (HTLV-I)-bearing MT-4 RT cells with HTLV-III (AIDS virus): chronological studies of early RT events."; RL Virology 146:272-281(1985). // RX CelloPub=CLPUB00542; RA Miyoshi, Isao RA Taguchi, Hirokuni RA Kubonishi, Ichiro RA Yoshimoto, Shizuo RA Ohtsuki, Yuji RA Shiraishi, Yukimasa RA Akagi, Tadaatsu RT "Type C virus-producing cell lines derived from adult T cell leukemia."; RL Gann Monogr. Cancer Res. 28:219-228(1982). // RX PubMed=9261343; DOI=10.1128/JVI.71.9.6277-6278.1997; PMCID=PMC191899; RA Jeang, Kuan-Teh RA Derse, David Daniel RA Matocha, Martha RA Sharma, Opendra RT "Expression status of Tax protein in human T-cell leukemia virus type RT 1-transformed MT4 cells: recall of MT4 cells distributed by the NIH RT AIDS Research and Reference Reagent Program."; RL J. Virol. 71:6277-6278(1997). // RX PubMed=31554688; DOI=10.1128/JVI.01390-19; PMCID=PMC6880167; RA Fernandez, Melissa Victoria RA Delviks-Frankenberry, Krista A. RA Scheiblin, David A. RA Happel, Christine RA Pathak, Vinay K. RA Freed, Eric O. RT "Authentication analysis of MT-4 cells distributed by the National RT Institutes of Health AIDS Reagent Program."; RL J. Virol. 93:e01390.19.1-e01390.19.8(2019). // RX PubMed=8927062; RA Susloparov, Mikhail Alexandrovich RA Krendel'shchikov, A.V. RA Bakhtina, Marina M. RA Bedristov, A.I. RA Babkin, Igor V. RA Gutorov, Valery V. RA Eroshkin, Alexey M. RA Mamaeva, Olga A. RT "Cloning of proviral DNA sequences of the human T-cell leukemia virus RT type 1 (HTLV-1) from the genome of cultured MT-4 cells."; RL Mol. Gen. Mikrobiol. Virusol. 1996:32-39(1996). // RX PubMed=1364232; RA Gyuris, Agnes RA Vajda, Gabor RA Foldes, Istvan RT "Establishment of an MT4 cell line persistently producing infective RT HIV-1 particles."; RL Acta Microbiol. Hung. 39:271-279(1992). // RX PubMed=32938764; DOI=10.1128/JVI.01334-20; PMCID=PMC7654282; RA Fernandez, Melissa Victoria RA Hoffman, Huxley K. RA Pezeshkian, Nairi RA Tedbury, Philip R. RA van Engelenburg, Schuyler B. RA Freed, Eric O. RT "Elucidating the basis for permissivity of the MT-4 T-cell line to RT replication of an HIV-1 mutant lacking the gp41 cytoplasmic tail."; RL J. Virol. 94:e01334.20.1-e01334.20.27(2020). // RX PubMed=8957066; DOI=10.1111/j.1349-7006.1996.tb03112.x; PMCID=PMC5920998; RA Kawasaki, Nobuhiro RA Matsuo, Yoshinobu RA Yoshino, Tadashi RA Yanai, Hiroyuki RA Oka, Takashi RA Teramoto, Norihiro RA Liu, Cao RA Kondo, Eisaku RA Minowada, Jun RA Akagi, Tadaatsu RT "Metastatic potential of lymphoma/leukemia cell lines in SCID mice is RT closely related to expression of CD44."; RL Jpn. J. Cancer Res. 87:1070-1077(1996). //