ID   MT-4
AC   CVCL_2632
SY   MT4
DR   BTO; BTO_0003030
DR   CLO; CLO_0007891
DR   CLDB; cl3594
DR   CLDB; cl3599
DR   BEI_Resources; ARP-120
DR   BioSample; SAMN03470803
DR   BioSample; SAMN03472442
DR   ChEMBL-Cells; CHEMBL3308375
DR   ChEMBL-Targets; CHEMBL388
DR   Cosmic; 1191697
DR   ECACC; 08081402
DR   JCRB; JCRB0135
DR   JCRB; JCRB1216
DR   JCRB; NIHS0145
DR   JCRB; NIHS0529
DR   KCB; KCB 200308YJ
DR   PubChem_Cell_line; CVCL_2632
DR   Wikidata; Q54906940
RX   CelloPub=CLPUB00542;
RX   PubMed=1364232;
RX   PubMed=1765142;
RX   PubMed=2413616;
RX   PubMed=8927062;
RX   PubMed=8957066;
RX   PubMed=9261343;
RX   PubMed=29892436;
RX   PubMed=31554688;
RX   PubMed=32938764;
WW   Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/m/cell-lines-detail-117.html
CC   Problematic cell line: Partially contaminated. Two different lots formerly distributed by NIH-ARP; 13 P7 3/9/92 and 150048; were shown not to be authentic MT-4 (PubMed=9261343; PubMed=31554688).
CC   Population: Japanese.
CC   Characteristics: Was established by co-cultivation of cells from a 50 year old male T-cell leukemia patient with cord leukocytes from a male infant (CelloPub=CLPUB00542).
CC   Virology: HTLV-1 producing cell line. Expresses Tax. Is permissive for replication of an HIV-1 gp41 mutant lacking the cytoplasmic tail.
CC   Doubling time: ~30 hours (Note=Lot 102799) (JCRB=JCRB1216).
CC   Transformant: NCBI_TaxID; 11908; Human T-lymphotropic virus 1 (HTLV-1).
CC   Discontinued: JCRB; JCRB0135; probable.
CC   Discontinued: JCRB; JCRB1216; probable.
CC   Discontinued: JCRB; NIHS0145; true.
CC   Discontinued: JCRB; NIHS0529; true.
CC   Derived from site: In situ; Umbilical cord blood; UBERON=UBERON_0012168.
CC   Cell type: T-cell; CL=CL_0000084.
ST   Source(s): JCRB=JCRB0135; JCRB=JCRB1216; PubMed=31554688; PubMed=32938764
ST   Amelogenin: X,Y
ST   CSF1PO: 11,12
ST   D13S317: 12
ST   D16S539: 9,12
ST   D18S51: 13
ST   D21S11: 28,30.3
ST   D3S1358: 17
ST   D5S818: 10,11
ST   D7S820: 8,9.3,10 (JCRB=JCRB1216)
ST   D7S820: 8,10 (JCRB=JCRB0135; PubMed=31554688; PubMed=32938764)
ST   D8S1179: 10,15
ST   FGA: 23
ST   Penta D: 10,13
ST   Penta E: 5,15
ST   TH01: 7
ST   TPOX: 11
ST   vWA: 17,18
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
SX   Male
AG   <1D
CA   Transformed cell line
DT   Created: 04-04-12; Last updated: 10-04-25; Version: 27
//
RX   CelloPub=CLPUB00542;
RA   Miyoshi, Isao
RA   Taguchi, Hirokuni
RA   Kubonishi, Ichiro
RA   Yoshimoto, Shizuo
RA   Ohtsuki, Yuji
RA   Shiraishi, Yukimasa
RA   Akagi, Tadaatsu
RT   "Type C virus-producing cell lines derived from adult T cell leukemia.";
RL   Gann Monogr. Cancer Res. 28:219-228(1982).
//
RX   PubMed=1364232;
RA   Gyuris, Agnes
RA   Vajda, Gabor
RA   Foldes, Istvan
RT   "Establishment of an MT4 cell line persistently producing infective
RT   HIV-1 particles.";
RL   Acta Microbiol. Hung. 39:271-279(1992).
//
RX   PubMed=1765142; DOI=10.1016/0014-5793(91)81402-t;
RA   Orita, Satoshi
RA   Saiga, Akihiko
RA   Takagi, Shin
RA   Tanaka, Tatsuro
RA   Okumura, Koichi
RA   Aono, Yuko
RA   Hinuma, Yorio
RA   Igarashi, Hisanaga
RT   "A novel alternatively spliced viral mRNA transcribed in cells
RT   infected with human T cell leukemia virus type 1 is mainly responsible
RT   for expressing p21X protein.";
RL   FEBS Lett. 295:127-134(1991).
//
RX   PubMed=2413616; DOI=10.1016/0042-6822(85)90010-8;
RA   Harada, Shinji
RA   Koyanagi, Yoshio
RA   Yamamoto, Naoki
RT   "Infection of human T-lymphotropic virus type-I (HTLV-I)-bearing MT-4
RT   cells with HTLV-III (AIDS virus): chronological studies of early
RT   events.";
RL   Virology 146:272-281(1985).
//
RX   PubMed=8927062;
RA   Susloparov, Mikhail Alexandrovich
RA   Krendel'shchikov, A.V.
RA   Bakhtina, Marina M.
RA   Bedristov, A.I.
RA   Babkin, Igor V.
RA   Gutorov, Valery V.
RA   Eroshkin, Alexey M.
RA   Mamaeva, Olga A.
RT   "Cloning of proviral DNA sequences of the human T-cell leukemia virus
RT   type 1 (HTLV-1) from the genome of cultured MT-4 cells.";
RL   Mol. Gen. Mikrobiol. Virusol. 1996:32-39(1996).
//
RX   PubMed=8957066; DOI=10.1111/j.1349-7006.1996.tb03112.x; PMCID=PMC5920998;
RA   Kawasaki, Nobuhiro
RA   Matsuo, Yoshinobu
RA   Yoshino, Tadashi
RA   Yanai, Hiroyuki
RA   Oka, Takashi
RA   Teramoto, Norihiro
RA   Liu, Cao
RA   Kondo, Eisaku
RA   Minowada, Jun
RA   Akagi, Tadaatsu
RT   "Metastatic potential of lymphoma/leukemia cell lines in SCID mice is
RT   closely related to expression of CD44.";
RL   Jpn. J. Cancer Res. 87:1070-1077(1996).
//
RX   PubMed=9261343; DOI=10.1128/JVI.71.9.6277-6278.1997; PMCID=PMC191899;
RA   Jeang, Kuan-Teh
RA   Derse, David Daniel
RA   Matocha, Martha
RA   Sharma, Opendra
RT   "Expression status of Tax protein in human T-cell leukemia virus type
RT   1-transformed MT4 cells: recall of MT4 cells distributed by the NIH
RT   AIDS Research and Reference Reagent Program.";
RL   J. Virol. 71:6277-6278(1997).
//
RX   PubMed=29892436; DOI=10.1098/rsos.172472; PMCID=PMC5990783;
RA   Shioda, Setsuko
RA   Kasai, Fumio
RA   Watanabe, Ken
RA   Kawakami, Kohei
RA   Ohtani, Azusa
RA   Iemura, Masashi
RA   Ozawa, Midori
RA   Arakawa, Akemi
RA   Hirayama, Noriko
RA   Kawaguchi, Eiko
RA   Tano, Tomoko
RA   Miyata, Sayaka
RA   Satoh, Motonobu
RA   Shimizu, Norio
RA   Kohara, Arihiro
RT   "Screening for 15 pathogenic viruses in human cell lines registered at
RT   the JCRB Cell Bank: characterization of in vitro human cells by viral
RT   infection.";
RL   R. Soc. Open Sci. 5:172472-172472(2018).
//
RX   PubMed=31554688; DOI=10.1128/JVI.01390-19; PMCID=PMC6880167;
RA   Fernandez, Melissa Victoria
RA   Delviks-Frankenberry, Krista A.
RA   Scheiblin, David A.
RA   Happel, Christine
RA   Pathak, Vinay K.
RA   Freed, Eric O.
RT   "Authentication analysis of MT-4 cells distributed by the National
RT   Institutes of Health AIDS Reagent Program.";
RL   J. Virol. 93:e01390.19.1-e01390.19.8(2019).
//
RX   PubMed=32938764; DOI=10.1128/JVI.01334-20; PMCID=PMC7654282;
RA   Fernandez, Melissa Victoria
RA   Hoffman, Huxley K.
RA   Pezeshkian, Nairi
RA   Tedbury, Philip R.
RA   van Engelenburg, Schuyler B.
RA   Freed, Eric O.
RT   "Elucidating the basis for permissivity of the MT-4 T-cell line to
RT   replication of an HIV-1 mutant lacking the gp41 cytoplasmic tail.";
RL   J. Virol. 94:e01334.20.1-e01334.20.27(2020).
//