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Cellosaurus Ej138 (CVCL_2443)

[Text version]
Cell line name Ej138
Synonyms EJ138; EJ 138; MGH-U1; MGHU1; MGH-UI; MGH-U1 (EJ); EJ
Accession CVCL_2443
Resource Identification Initiative To cite this cell line use: Ej138 (RRID:CVCL_2443)
Comments Problematic cell line: Contaminated. Shown to be a T24 derivative (PubMed=3708594; PubMed=6823318; PubMed=20143388).
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00080.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00195.
Population: Caucasian; Swedish.
Doubling time: 20 hours (PubMed=3708594).
Omics: Transcriptomics; ESTs sequencing.
Omics: Transcriptomics; Microarray.
Omics: Variations; SNP array analysis.
Anecdotal: This is the cell line where the first human oncogene, HRAS was first discovered (PubMed=6286138).
Derived from site: In situ; Urinary bladder; UBERON=UBERON_0001255.
Sequence variations
  • Mutation; HGNC; HGNC:5173; HRAS; Simple; p.Gly12Val (c.35G>T); ClinVar=VCV000012600; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Tyr126Ter (c.378C>G); ClinVar=VCV000578988; Zygosity=Homozygous (from parent cell line).
Disease Bladder carcinoma (NCIt: C4912)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0554 (T24)
Children:
CVCL_LN34 (EJ-30)CVCL_LN38 (MGH-U1/OCI)
Sex of cell Female
Age at sampling 82Y
Category Cancer cell line
STR profile Source(s): PubMed=11416159

Markers:
AmelogeninX
D8S117914
D18S5116,18
D21S1129
FGA22
TH016
vWA17

Run an STR similarity search on this cell line
Web pages Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Publications

PubMed=864752; DOI=10.1093/jnci/58.6.1743
Christopher John Marshall, Leonard Maurice Franks, Anthony W. Carbonell;
Markers of neoplastic transformation in epithelial cell lines derived from human carcinomas.
J. Natl. Cancer Inst. 58:1743-1751(1977)

PubMed=870558; DOI=10.1177/25.4.870558
Frances J. Benham, David C. Cottell, Leonard Maurice Franks, Patricia D. Wilson;
Alkaline phosphatase activity in human bladder tumor cell lines.
J. Histochem. Cytochem. 25:266-274(1977)

PubMed=651066; DOI=10.5980/jpnjurol1928.69.1_40
Tetsuro Kato, Kiyoshi Ishikawa, Ryosuke Nemoto;
Morphologic characterization of two established cell lines, T24 and MGH-U1, derived from human bladder carcinoma.
Nihon Hinyokika Gakkai Zasshi 69:40-46(1978)

PubMed=663932; DOI=10.1620/tjem.124.33
Tetsuro Kato, Kiyoshi Ishikawa, Ryosuke Nemoto, Akira Senoo, Yasuji Amano;
Morphological characterization of two established cell lines, T24 and MGH-U1, derived from human urinary bladder carcinoma.
Tohoku J. Exp. Med. 124:339-349(1978)

PubMed=6244232
Richard D. Williams;
Human urologic cancer cell lines.
Invest. Urol. 17:359-363(1980)

PubMed=6286138; DOI=10.1016/0092-8674(82)90100-3
Chiaho Shih, Robert Allan Weinberg;
Isolation of a transforming sequence from a human bladder carcinoma cell line.
Cell 29:161-169(1982)

PubMed=7185004; DOI=10.2302/kjm.31.127
Masaaki Tachibana;
Studies on cellular adhesiveness in five different culture cell lines derived from carcinoma of the urinary bladder.
Keio J. Med. 31:127-148(1982)

PubMed=6823318; DOI=10.1038/301429a0
Carol M. O'Toole, Susan Povey, Peter J. Hepburn, Leonard Maurice Franks;
Identity of some human bladder cancer cell lines.
Nature 301:429-430(1983)

PubMed=6826254; DOI=10.1002/ijc.2910310308
Staffan Paulie, Yngve Hansson, Marie-Louise Lundblad, Peter Perlmann;
Lectins as probes for identification of tumor-associated antigens on urothelial and colonic carcinoma cell lines.
Int. J. Cancer 31:297-303(1983)

PubMed=4027986
Chi-Wei Lin, Julia C. Lin, George Russell Prout Jr.;
Establishment and characterization of four human bladder tumor cell lines and sublines with different degrees of malignancy.
Cancer Res. 45:5070-5079(1985)

PubMed=3708594
John R.W. Masters, Peter J. Hepburn, Lawrence Walker, Wilma J. Highman, Ludwik K. Trejdosiewicz, Susan Povey, Mohamed Parkar, Bridget T. Hill, Peter N. Riddle, Leonard Maurice Franks;
Tissue culture model of transitional cell carcinoma: characterization of twenty-two human urothelial cell lines.
Cancer Res. 46:3630-3636(1986)

PubMed=2903891; DOI=10.1080/09553008814552341
John Hollis Peacock, Anna M. Cassoni, Trevor J. McMillan, George Gordon Steel;
Radiosensitive human tumour cell lines may not be recovery deficient.
Int. J. Radiat. Biol. 54:945-953(1988)

PubMed=11416159; DOI=10.1073/pnas.121616198; PMCID=PMC35459
John R.W. Masters, Jim A. Thomson, Bernadette Daly-Burns, Yvonne A. Reid, Wilhelm Gerhard Dirks, Phil Packer, Lorraine H. Toji, Tadao Ohno, Hideyuki Tanabe, Colin Francis Arlett, Lloyd R. Kelland ...Show all 16 authors... , Maureen Harrison, Arvind K. Virmani, Timothy H. Ward, Karen L. Ayres, Paul G. Debenham; Show fewer authors
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

PubMed=20143388; DOI=10.1002/ijc.25242
Amanda Capes-Davis, George Theodosopoulos, Isobel Atkin, Hans Gunther Drexler, Arihiro Kohara, Roderick A.F. MacLeod, John R.W. Masters, Yukio Nakamura, Yvonne A. Reid, Roger Robert Reddel, Robert Ian Freshney;
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=23401075; DOI=10.1002/path.4176
Ya-Nan Guo, Yvonne Chekaluk, Jian-Ming Zhang, Jin-Yan Du, Nathanael S. Gray, Chin-Lee Wu, David Joseph Kwiatkowski;
TSC1 involvement in bladder cancer: diverse effects and therapeutic implications.
J. Pathol. 230:17-27(2013)

PubMed=25336970; DOI=10.2147/OTT.S69239; PMCID=PMC4199822
Pei-Shan Yang, Yu-Chao Hsu, Yu-Hsiang Lin, Cheng-Pang Hou, Chien-Lun Chen, Phei-Lang Chang, Horng-Heng Juang, Ke-Hung Tsui;
The expression sequence tag is an effective method for screening DNA segments that predict urinary bladder transitional cell carcinoma prognosis.
Onco Targets Ther. 7:1777-1781(2014)

Cross-references
Cell line collections (Providers) ECACC; 85061108
NCBI_Iran; C429
Cell line databases/resources CLO; CLO_0002905
CLO; CLO_0002906
CLDB; cl1152
CLDB; cl1154
cancercelllines; CVCL_2443
LINCS_LDP; LCL-1704
Anatomy/cell type resources BTO; BTO_0003553
Biological sample resources BioSample; SAMN03151939
BioSample; SAMN03151949
Chemistry resources ChEMBL-Cells; CHEMBL3307583
ChEMBL-Targets; CHEMBL614292
PubChem_Cell_line; CVCL_2443
Encyclopedic resources Wikidata; Q54832176
Experimental variables resources EFO; EFO_0022418
Gene expression databases GEO; GSM827443
GEO; GSM1374473
Polymorphism and mutation databases Cosmic; 760480
Cosmic; 925828
Cosmic; 1093986
Cosmic; 1927289
Progenetix; CVCL_2443
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number37