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Cellosaurus CRFK (CVCL_2426)

[Text version]
Cell line name CRFK
Synonyms CrFK; Crfk; NBL-CrFK; Crandell Reese Feline Kidney; CFK; Crandell Feline Kidney; CCC; Crandell's Cat Cell
Accession CVCL_2426
Resource Identification Initiative To cite this cell line use: CRFK (RRID:CVCL_2426)
Comments Group: Vaccine production cell line.
Part of: Naval Biosciences Laboratory (NBL) collection (transferred to ATCC in 1982).
Biotechnology: Widely used to manufacture vaccines for parvoviruses such as feline panleukopenia virus (FPLV) and canine parvoviruses (CPVs).
Virology: Persistently infected by feline endogenous virus RD-114.
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
Species of origin Felis catus (Cat) (Felis silvestris catus) (NCBI Taxonomy: 9685)
Hierarchy Parent: CVCL_DB45 (FCK)
CVCL_W183 (CCC3a subline 8C)CVCL_X693 (CRFK/FIV-AZR-1)CVCL_WL54 (Teth-CRFK)
Sex of cell Female
Age at sampling 3M
Category Spontaneously immortalized cell line
Web pages https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/c/cell-lines-detail-435.html

PubMed=4130570; DOI=10.1007/BF02618435
Crandell R.A., Fabricant C.G., Nelson-Rees W.A.
Development, characterization, and viral susceptibility of a feline (Felis catus) renal cell line (CRFK).
In Vitro 9:176-185(1973)

PubMed=75945; DOI=10.1099/0022-1317-38-3-483
O'Brien S.J., Simonson J.M., Davis S.
Deposition of retrovirus associated antigens (p30 and gp70) on cell membranes of feline and murine leukaemia virus infected cells.
J. Gen. Virol. 38:483-496(1978)

Fulton R.W., Pearson N.J.
Interferon production by bovine tracheal organ cultures infected with bovid herpesvirus 1 strains.
Can. J. Comp. Med. 44:447-452(1980)

PubMed=1313703; DOI=10.1292/jvms.54.173
Miyazawa T., Toyosaki T., Tomonaga K., Norimine J., Ohno K., Hasegawa A., Kai C., Mikami T.
Further characterization of a feline T-lymphoblastoid cell line (MYA-1 cells) highly sensitive for feline immunodeficiency virus.
J. Vet. Med. Sci. 54:173-175(1992)

PubMed=9696876; DOI=10.1128/JVI.72.9.7685-7687.1998
Baumann J.G., Gunzburg W.H., Salmons B.
CrFK feline kidney cells produce an RD114-like endogenous virus that can package murine leukemia virus-based vectors.
J. Virol. 72:7685-7687(1998)

PubMed=20631117; DOI=10.1128/jcm.00992-10
Yoshikawa R., Sato E., Igarashi T., Miyazawa T.
Characterization of RD-114 virus isolated from a commercial canine vaccine manufactured using CRFK cells.
J. Clin. Microbiol. 48:3366-3369(2010)

PubMed=21029758; DOI=10.1016/j.virusres.2010.10.020
Okada M., Yoshikawa R., Shojima T., Baba K., Miyazawa T.
Susceptibility and production of a feline endogenous retrovirus (RD-114 virus) in various feline cell lines.
Virus Res. 155:268-273(2011)

PubMed=23585909; DOI=10.1371/journal.pone.0061530
Fukuma A., Yoshikawa R., Miyazawa T., Yasuda J.
A new approach to establish a cell line with reduced risk of endogenous retroviruses.
PLoS ONE 8:E61530-E61530(2013)

Cell line collections (Providers) ATCC; CCL-94
ATCC; CRL-6073 - Discontinued
BCRC; 60151
BCRJ; 0072
ECACC; 86093002
KCB; KCB 200974YJ
KCLB; 10094
Cell line databases/resources CLO; CLO_0002609
CLDB; cl907
CLDB; cl908
Lonza; 801
TOKU-E; 997
Encyclopedic resources Wikidata; Q54814484
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number30