Cell line name |
ATRFLOX |
Synonyms |
ATRFLOX [Mutatect]; ATRflox/- |
Accession |
CVCL_2299 |
Resource Identification Initiative |
To cite this cell line use: ATRFLOX (RRID:CVCL_2299) |
Comments |
Characteristics: One copy of the ATR gene has been disrupted while lox sites have been added flanking exon 2 of the 2nd allele thus making it susceptible to Cre-mediated deletion. Knockout cell: Method=Homologous recombination (1 of 2 alleles); HGNC; 882; ATR. Omics: Deep exome analysis. Omics: Deep RNAseq analysis. Omics: SNP array analysis. Derived from sampling site: Colon. |
Sequence variations |
- Mutation; HGNC; 173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Homozygous (from parent cell line).
- Mutation; HGNC; 1101; BRCA2; Simple; p.Ile2675Aspfs*6 (c.8021dupA) (c.8021_8022insA); ClinVar=VCV000267050; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; 1787; CDKN2A; Simple; p.Arg24Serfs*20 (c.68dupG) (c.68_69insG) (p.G23fs); Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; 2514; CTNNB1; Simple; p.Ser45del (c.133_135delTCT); ClinVar=VCV000017576; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; 3373; EP300; Simple; p.Met1470Cysfs*22 (c.4408delA); Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; 3373; EP300; Simple; p.Asn1700Thrfs*9 (c.5099delA); Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; 6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; 8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Heterozygous (from parent cell line).
- Mutation; HGNC; 11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Homozygous (from parent cell line).
|
Disease |
Colon carcinoma (NCIt: C4910) |
Species of origin |
Homo sapiens (Human)
(NCBI Taxonomy: 9606) |
Hierarchy |
Parent: CVCL_0291 (HCT 116) |
Sex of cell |
Male |
Age at sampling |
48Y |
Category |
Cancer cell line |
STR profile |
Source(s): ATCC; PubMed=25877200
Markers:Amelogenin | X |
CSF1PO | 7,9 |
D3S1358 | 12,19 |
D5S818 | 10,12 |
D7S820 | 11,12 |
D8S1179 | 12,14 |
D13S317 | 10,12 |
D16S539 | 11,13 |
D18S51 | 16,17 |
D21S11 | 29,30 |
FGA | 18,23 |
Penta D | 9,13 |
Penta E | 13,14 |
TH01 | 8,9 |
TPOX | 8 |
vWA | 17,22 |
Run an STR similarity search on this cell line |
Publications | PubMed=11721054; DOI=10.1126/science.1065521 Cortez D., Guntuku S., Qin J., Elledge S.J. ATR and ATRIP: partners in checkpoint signaling. Science 294:1713-1716(2001) PubMed=25485619; DOI=10.1038/nbt.3080 Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M. A comprehensive transcriptional portrait of human cancer cell lines. Nat. Biotechnol. 33:306-312(2015) |
Cross-references |
Cell line collections |
ATCC; CRL-2780
|
Cell line databases/resources |
CLO; CLO_0001764
ColonAtlas; ATRFLOX
|
Biological sample resources |
BioSample; SAMN03472971
|
Chemistry resources |
PharmacoDB; ATRFLOX_69_2019
|
Encyclopedic resources |
Wikidata; Q54751289
|
Experimental variables resources |
EFO; EFO_0006535
|
Gene expression databases |
ArrayExpress; E-MTAB-2706
|
Sequence databases |
EGA; EGAS00001000610
EGA; EGAS00001002554
|
Entry history |
Entry creation | 04-Apr-2012 |
Last entry update | 23-Jun-2022 |
Version number | 23 |
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