ID   ATRFLOX
AC   CVCL_2299
SY   ATRFLOX [Mutatect]; ATRflox/-
DR   CLO; CLO_0001764
DR   EFO; EFO_0006535
DR   ArrayExpress; E-MTAB-2706
DR   ATCC; CRL-2780
DR   BioSample; SAMN03472971
DR   cancercelllines; CVCL_2299
DR   ColonAtlas; ATRFLOX
DR   EGA; EGAS00001000610
DR   EGA; EGAS00001002554
DR   PharmacoDB; ATRFLOX_69_2019
DR   Wikidata; Q54751289
RX   PubMed=11721054;
RX   PubMed=25485619;
RX   PubMed=25877200;
RX   PubMed=26589293;
CC   Population: Caucasian.
CC   Characteristics: One copy of the ATR gene has been disrupted while lox sites have been added flanking exon 2 of the 2nd allele thus making it susceptible to Cre-mediated deletion.
CC   HLA typing: A*01:01,02:01; B*18:01,45:01; C*05:01,07:06 (PubMed=26589293).
CC   Knockout cell: Method=Homologous recombination; HGNC; 882; ATR (Note=1 of 2 alleles).
CC   Sequence variation: Mutation; HGNC; 173; ACVR2A; Simple; p.Lys437Argfs*5 (c.1310delA); dbSNP=rs764719749; Zygosity=Homozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 1101; BRCA2; Simple; p.Ile2675Aspfs*6 (c.8021dupA) (c.8021_8022insA); ClinVar=VCV000267050; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 1787; CDKN2A; Simple; p.Arg24Serfs*20 (c.68dupG) (c.68_69insG) (p.G23fs); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 2514; CTNNB1; Simple; p.Ser45del (c.133_135delTCT); ClinVar=VCV000017576; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 3373; EP300; Simple; p.Met1470Cysfs*22 (c.4408delA); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 3373; EP300; Simple; p.Asn1700Thrfs*9 (c.5099delA); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 6407; KRAS; Simple; p.Gly13Asp (c.38G>A); ClinVar=VCV000012580; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 8975; PIK3CA; Simple; p.His1047Arg (c.3140A>G); ClinVar=VCV000013652; Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 9277; PPM1D; Simple; p.Leu450Ter (c.1349delT) (p.Leu450fs) (c.1344delT); Zygosity=Heterozygous (from parent cell line).
CC   Sequence variation: Mutation; HGNC; 11773; TGFBR2; Simple; p.Lys128Serfs*35 (c.383delA); ClinVar=VCV000477546; Zygosity=Homozygous (from parent cell line).
CC   Omics: Deep exome analysis.
CC   Omics: SNP array analysis.
CC   Omics: Transcriptome analysis by RNAseq.
CC   Derived from site: In situ; Colon; UBERON=UBERON_0001155.
ST   Source(s): ATCC; PubMed=25877200
ST   Amelogenin: X
ST   CSF1PO: 7,9
ST   D13S317: 10,12
ST   D16S539: 11,13
ST   D18S51: 16,17
ST   D21S11: 29,30
ST   D3S1358: 12,19
ST   D5S818: 10,12
ST   D7S820: 11,12
ST   D8S1179: 12,14
ST   FGA: 18,23
ST   Penta D: 9,13
ST   Penta E: 13,14
ST   TH01: 8,9
ST   TPOX: 8
ST   vWA: 17,22
DI   NCIt; C4910; Colon carcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0291 ! HCT 116
SX   Male
AG   48Y
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 30-01-24; Version: 27
//
RX   PubMed=11721054; DOI=10.1126/science.1065521;
RA   Cortez D., Guntuku S., Qin J., Elledge S.J.;
RT   "ATR and ATRIP: partners in checkpoint signaling.";
RL   Science 294:1713-1716(2001).
//
RX   PubMed=25485619; DOI=10.1038/nbt.3080;
RA   Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S.,
RA   Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G.,
RA   Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J.,
RA   Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L.,
RA   Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J.,
RA   Settleman J., Seshagiri S., Zhang Z.-M.;
RT   "A comprehensive transcriptional portrait of human cancer cell
RT   lines.";
RL   Nat. Biotechnol. 33:306-312(2015).
//
RX   PubMed=25877200; DOI=10.1038/nature14397;
RA   Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M.,
RA   Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S.,
RA   Neve R.M.;
RT   "A resource for cell line authentication, annotation and quality
RT   control.";
RL   Nature 520:307-311(2015).
//
RX   PubMed=26589293; DOI=10.1186/s13073-015-0240-5;
RA   Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P.,
RA   Loewer M., Sahin U., Castle J.C.;
RT   "TCLP: an online cancer cell line catalogue integrating HLA type,
RT   predicted neo-epitopes, virus and gene expression.";
RL   Genome Med. 7:118.1-118.7(2015).
//