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Cellosaurus U-373MG ATCC (CVCL_2219)

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Cell line name U-373MG ATCC
Synonyms U-373MG; U373 MG; U-373-MG; U-373 MG; U373-MG; U373MG; U373; 373 MG; 373MG
Accession CVCL_2219
Resource Identification Initiative To cite this cell line use: U-373MG ATCC (RRID:CVCL_2219)
Comments Problematic cell line: Misidentified/contaminated. Shown to be a U-251MG derivative. This cell line is not the original astrocytoma cell line established in 1973 at the University of Uppsala. See U-373MG Uppsala (Cellosaurus=CVCL_2818) for the original U-373MG cell line.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00295.
Population: Caucasian.
Doubling time: 40 hours (PubMed=9842975).
Omics: Genomics; Whole exome sequencing.
Omics: Metabolomics.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; Array-based CGH.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Brain, parietal lobe; UBERON=UBERON_0001872.
Sequence variations
  • Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Glu242Valfs*15 (c.723_724dupTG); ClinVar=VCV000536553; Zygosity=Unspecified (from parent cell line).
  • Mutation; HGNC; HGNC:11730; TERT; Simple; c.1-124C>T (c.228C>T) (C228T); Zygosity=Unspecified; Note=In promoter (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Homozygous (PubMed=10416987; PubMed=14614447).
Disease Astrocytoma (NCIt: C60781)
Astrocytoma (ORDO: Orphanet_94)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0021 (U-251MG)
Children:
CVCL_E8AN (U373-CD4)
Sex of cell Male
Age at sampling 75Y
Category Cancer cell line
STR profile Source(s): ATCC=HTB-17; JCRB=NIHS0295; KCLB=30017; PubMed=22570425

Markers:
AmelogeninX,Y
CSF1PO11,12
D3S135816,17
D5S81811,12
D7S82010,12
D8S117913,15
D13S31710,11
D16S53912
D18S5113
D21S1129,30
FGA21,25
Penta D10,12
Penta E7,10
TH019.3 (ATCC=HTB-17; KCLB=30017; PubMed=22570425)
9.3,11 (JCRB=NIHS0295)
TPOX8
vWA16,18

Run an STR similarity search on this cell line
Web pages Info; ATCC; -; https://www.atcc.org/en/support/technical-support/faqs/u-373-mg-atcc-htb-17
Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Info; StRAP; -; https://strap.nci.nih.gov/celline_detail.php?sample_id=76
Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/u/cell-lines-detail-510.html
Provider; NIBSC; 0029; http://www.nibsc.org/Default.aspx?pageid=597&id=0029&theme=default
Publications

PubMed=833871; DOI=10.1093/jnci/58.2.209
Jorgen Fogh, William C. Wright, James D. Loveless;
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)

PubMed=450131; DOI=10.1038/279797a0
Rufus S. Day 3rd, Chuck H.J. Ziolkowski;
Human brain tumour cell strains with deficient host-cell reactivation of N-methyl-N'-nitro-N-nitrosoguanidine-damaged adenovirus 5.
Nature 279:797-799(1979)

PubMed=22282976; DOI=10.1093/carcin/1.1.21
Rufus S. Day 3rd, Chuck H.J. Ziolkowski, Dominic A. Scudiero, Sharon A. Meyer, Michael R. Mattern;
Human tumor cell strains defective in the repair of alkylation damage.
Carcinogenesis 1:21-32(1980)

PubMed=6260907; DOI=10.1097/00005072-198105000-00001
Darell Doty Bigner, Sandra H. Bigner, Jan Ponten, Bengt Westermark, Moses Stephen Mahaley Jr., Erkki Ruoslahti, Harvey R. Herschman, Lawrence F. Eng, Carol J. Wikstrand;
Heterogeneity of genotypic and phenotypic characteristics of fifteen permanent cell lines derived from human gliomas.
J. Neuropathol. Exp. Neurol. 40:201-229(1981)

PubMed=6220172
Nicholas C. Dracopoli, Jorgen Fogh;
Polymorphic enzyme analysis of cultured human tumor cell lines.
J. Natl. Cancer Inst. 70:469-476(1983)

PubMed=6582512; DOI=10.1073/pnas.81.2.568; PMCID=PMC344720
Michael Jules Mattes, Carlos Cordon-Cardo, John L. Lewis Jr., Lloyd John Old, Kenneth Oliver Lloyd;
Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.
Proc. Natl. Acad. Sci. U.S.A. 81:568-572(1984)

PubMed=3518877; DOI=10.3109/07357908609038260
Jorgen Fogh;
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=2253244
Sandra H. Bigner, Peter A. Humphrey, Albert J. Wong, Bert Vogelstein, Joachim Mark, Henry Seth Friedman, Darell Doty Bigner;
Characterization of the epidermal growth factor receptor in human glioma cell lines and xenografts.
Cancer Res. 50:8017-8022(1990)

PubMed=8858520; DOI=10.1007/BF00182138
Bertrand C. Liang;
Effects of hypoxia on drug resistance phenotype and genotype in human glioma cell lines.
J. Neurooncol. 29:149-155(1996)

PubMed=9220028; DOI=10.1016/S0303-7207(97)00080-4
Taraneh R. Sharif, Wei Luo, Mohammed Sharif;
Functional expression of bombesin receptor in most adult and pediatric human glioblastoma cell lines; role in mitogenesis and in stimulating the mitogen-activated protein kinase pathway.
Mol. Cell. Endocrinol. 130:119-130(1997)

PubMed=9230885; DOI=10.1093/jnci/89.14.1036
Candelaria Gomez-Manzano, Juan Fueyo, Athanassios P. Kyritsis, Timothy J. McDonnell, Peter A. Steck, Victor Alan Levin, Wai-Kwan Alfred Yung;
Characterization of p53 and p21 functional interactions in glioma cells en route to apoptosis.
J. Natl. Cancer Inst. 89:1036-1044(1997)

PubMed=9842975; DOI=10.1002/(SICI)1097-0215(19981218)79:6<640::AID-IJC15>3.0.CO;2-Z
Michael Weller, Johannes Rieger, Cornelia Grimmel, Erwin G. Van Meir, Nicolas De Tribolet, Stanislaw Krajewski, John C. Reed, Andreas von Deimling, Johannes Dichgans;
Predicting chemoresistance in human malignant glioma cells: the role of molecular genetic analyses.
Int. J. Cancer 79:640-644(1998)

PubMed=10074188; DOI=10.1128/JVI.73.4.3338-3350.1999; PMCID=PMC104098
Nathalie Arbour, Genevieve Cote, Claude Lachance, Marc Tardieu, Neil R. Cashman, Pierre J. Talbot;
Acute and persistent infection of human neural cell lines by human coronavirus OC43.
J. Virol. 73:3338-3350(1999)

PubMed=10402232; DOI=10.3892/ijo.15.2.237
Taraneh R. Sharif, Mohammed Sharif;
Overexpression of protein kinase C epsilon in astroglial brain tumor derived cell lines and primary tumor samples.
Int. J. Oncol. 15:237-243(1999)

PubMed=10416987; DOI=10.1111/j.1750-3639.1999.tb00536.x; PMCID=PMC8098486
Nobuaki Ishii, Daniel Maier, Adrian Merlo, Mitsuhiro Tada, Yutaka Sawamura, Annie-Claire Diserens, Erwin G. Van Meir;
Frequent co-alterations of TP53, p16/CDKN2A, p14ARF, PTEN tumor suppressor genes in human glioma cell lines.
Brain Pathol. 9:469-479(1999)

PubMed=11351043; DOI=10.1038/labinvest.3780280
Angela Bik-Yu Hui, Kwok-Wai Lo, Xiao-Lu Yin, Wai-Sang Poon, Ho-Keung Ng;
Detection of multiple gene amplifications in glioblastoma multiforme using array-based comparative genomic hybridization.
Lab. Invest. 81:717-723(2001)

PubMed=11414198; DOI=10.1007/s004320000207
Harald Lahm, Sabine Andre, Andreas Hoeflich, Jurgen R. Fischer, Bernard Sordat, Herbert Kaltner, Eckhard Wolf, Hans-Joachim Gabius;
Comprehensive galectin fingerprinting in a panel of 61 human tumor cell lines by RT-PCR and its implications for diagnostic and therapeutic procedures.
J. Cancer Res. Clin. Oncol. 127:375-386(2001)

PubMed=14614447; DOI=10.1038/sj.onc.1207198
Jorg Wischhusen, Ulrike Naumann, Hiroko Ohgaki, Farzan Rastinejad, Michael Weller;
CP-31398, a novel p53-stabilizing agent, induces p53-dependent and p53-independent glioma cell death.
Oncogene 22:8233-8245(2003)

PubMed=14655754; DOI=10.1111/j.1750-3639.2003.tb00479.x; PMCID=PMC8095903
Oliver Bahr, Johannes Rieger, Frank Duffner, Richard Meyermann, Michael Weller, Wolfgang Wick;
P-glycoprotein and multidrug resistance-associated protein mediate specific patterns of multidrug resistance in malignant glioma cell lines, but not in primary glioma cells.
Brain Pathol. 13:482-494(2003)

PubMed=16232199; DOI=10.1111/j.1349-7006.2005.00099.x; PMCID=PMC11159392
Kuniyasu Saigusa, Naoya Hashimoto, Hitoshi Tsuda, Sana Yokoi, Motohiko Maruno, Toshiki Yoshimine, Masaru Aoyagi, Kikuo Ohno, Issei Imoto, Johji Inazawa;
Overexpressed Skp2 within 5p amplification detected by array-based comparative genomic hybridization is associated with poor prognosis of glioblastomas.
Cancer Sci. 96:676-683(2005)

PubMed=16697959; DOI=10.1016/j.ccr.2006.03.030
Jeongwu Lee, Svetlana Kotliarova, Yuri Kotliarov, Ai-Guo Li, Qin Su, Nicholas Murray Donin, Sandra Pastorino, Benjamin W. Purow, Neil Christopher, Wei Zhang, John K. Park, Howard Alan Fine;
Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines.
Cancer Cell 9:391-403(2006)

PubMed=17595512; DOI=10.1159/000104150
Johannes Rieger, Brigitte Frank, Michael Weller, Wolfgang Wick;
Mechanisms of resistance of human glioma cells to Apo2 ligand/TNF-related apoptosis-inducing ligand.
Cell. Physiol. Biochem. 20:23-34(2007)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=20593219; DOI=10.1007/s11060-010-0283-9
Michael D. Blough, Desiree C. Beauchamp, Morgan R. Westgate, John J.P. Kelly, J. Gregory Cairncross;
Effect of aberrant p53 function on temozolomide sensitivity of glioma cell lines and brain tumor initiating cells from glioblastoma.
J. Neurooncol. 102:1-7(2011)

PubMed=21406405; DOI=10.1158/0008-5472.CAN-10-3112
Michal Grzmil, Pier Morin Jr., Maria Maddalena Lino, Adrian Merlo, Stephan Frank, Yu-Hua Wang, Gerald Moncayo, Brian Arthur Hemmings;
MAP kinase-interacting kinase 1 regulates SMAD2-dependent TGF-beta signaling pathway in human glioblastoma.
Cancer Res. 71:2392-2402(2011)

PubMed=22570425; DOI=10.1093/neuonc/nos072; PMCID=PMC3367844
Pierre Bady, Annie-Claire Diserens, Vincent Castella, Stefanie Kalt, Karl Heinimann, Marie-France Hamou, Mauro Delorenzi, Monika E. Hegi;
DNA fingerprinting of glioma cell lines and considerations on similarity measurements.
Neuro-oncol. 14:701-711(2012)

PubMed=26496030; DOI=10.18632/oncotarget.6171; PMCID=PMC4791243
Vikas Patil, Jagriti Pal, Kumaravel Somasundaram;
Elucidating the cancer-specific genetic alteration spectrum of glioblastoma derived cell lines from whole exome and RNA sequencing.
Oncotarget 6:43452-43471(2015)

Cross-references
Cell line collections (Providers) ATCC; HTB-17 - Discontinued
CCLV; CCLV-RIE 0675
ECACC; 89081403 - Discontinued
IZSLER; BS TCL 89
JCRB; NIHS0295 - Discontinued
KCLB; 30017
NCBI_Iran; C455
Cell line databases/resources CLO; CLO_0009460
CLO; CLO_0009461
CLDB; cl4587
CLDB; cl4589
CLDB; cl4964
cancercelllines; CVCL_2219
CGH-DB; 320-1
LINCS_LDP; LCL-1360
Lonza; 334
Lonza; 1006
TOKU-E; 3660
Anatomy/cell type resources BTO; BTO_0002126
Chemistry resources ChEMBL-Cells; CHEMBL3307474
ChEMBL-Cells; CHEMBL3886101
ChEMBL-Targets; CHEMBL615024
PubChem_Cell_line; CVCL_2219
Encyclopedic resources Wikidata; Q54973575
Experimental variables resources EFO; EFO_0001255
Gene expression databases GEO; GSM101681
GEO; GSM101682
GEO; GSM827314
GEO; GSM1638667
Metabolomic databases MetaboLights; MTBLS4708
Polymorphism and mutation databases Cosmic; 687589
Cosmic; 849849
Cosmic; 850825
Cosmic; 920819
Cosmic; 970093
Cosmic; 1066236
Cosmic; 1171226
Cosmic; 1175824
Cosmic; 1217674
Cosmic; 1294952
Cosmic; 1610742
Cosmic; 2367504
Cosmic; 2491105
Cosmic; 2701087
IARC_TP53; 2576
Progenetix; CVCL_2219
Proteomic databases PRIDE; PXD000430
PRIDE; PXD031569
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number39