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Cellosaurus SKNO-1 (CVCL_2196)

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Cell line name SKNO-1
Synonyms Skno-1; SKNO1
Accession CVCL_2196
Resource Identification Initiative To cite this cell line use: SKNO-1 (RRID:CVCL_2196)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: LL-100 blood cancer cell line panel.
Population: Japanese.
Characteristics: CSF2 dependent.
Doubling time: 48-72 hours (PubMed=7772516); 24 hours (PubMed=25984343); ~35-50 hours (DSMZ=ACC-690); ~38 hours (Note=Lot 06112012), 52-57 hours (Note=Lot 02032016) (JCRB=JCRB1170).
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; shRNA library screening.
Omics: Transcriptomics; RNAseq.
Derived from site: In situ; Bone marrow; UBERON=UBERON_0002371.
Sequence variations
  • Gene fusion; HGNC; HGNC:10471; RUNX1 + HGNC; HGNC:1535; RUNX1T1; Name(s)=RUNX1-RUNX1T1, AML1-ETO (PubMed=11753612; PubMed=15843827).
  • Mutation; HGNC; HGNC:6342; KIT; Simple; p.Asn822Lys (c.2466T>A) (N818K); ClinVar=VCV000375931; Zygosity=Homozygous (PubMed=18385756; DepMap=ACH-001656).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg248Gln (c.743G>A); ClinVar=VCV000012356; Zygosity=Homozygous (PubMed=7772516; DepMap=ACH-001656).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.His368Arg (c.1103G>A); Zygosity=Homozygous (PubMed=7772516; DepMap=ACH-001656).
HLA typing Source: DSMZCellDive=ACC-690
Class I
HLA-AA*02:06:01,31:01:02
HLA-BB*40:02:01,46:01:01
HLA-CC*01:02:01,03:04:01
Class II
HLA-DRDRA*01:01:01,01:02:02
DRB1*14:02:01,14:02:01
Genome ancestry Source: PubMed=30894373

Origin% genome
African1.83
Native American0
East Asian, North90.65
East Asian, South3.5
South Asian1.23
European, North0.2
European, South2.59
Disease Adult acute myeloid leukemia with maturation (NCIt: C7961)
Acute myeloblastic leukemia with maturation (ORDO: Orphanet_98834)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Male
Age at sampling 22Y
Category Cancer cell line
STR profile Source(s): DSMZ=ACC-690; JCRB=JCRB1170

Markers:
AmelogeninX
CSF1PO12,13
D2S133819
D3S135816
D5S81810,13
D7S82010,12
D8S117914,15
D13S3178,11
D16S53910,11
D18S5113,14
D19S43313,15
D21S1129,30
FGA23
Penta D9
Penta E11,12
TH017
TPOX8,9
vWA14,17

Run an STR similarity search on this cell line
Publications

PubMed=7772516; DOI=10.1111/j.1365-2141.1995.tb08418.x
Sachiko Matozaki, Toshitaro Nakagawa, Ryuji Kawaguchi, Riyoji Aozaki, Masayoshi Tsutsumi, Tohru Murayama, Tamio Koizumi, Ryuichiro Nishimura, Takashi Isobe, Kazuo Chihara;
Establishment of a myeloid leukaemic cell line (SKNO-1) from a patient with t(8;21) who acquired monosomy 17 during disease progression.
Br. J. Haematol. 89:805-811(1995)

PubMed=9738977; DOI=10.1111/j.1349-7006.1998.tb03275.x; PMCID=PMC5921886
Jun Takizawa, Ritsuro Suzuki, Hiroyuki Kuroda, Atae Utsunomiya, Yoshitoyo Kagami, Tatsuroh Joh, Yoshifusa Aizawa, Ryuzo Ueda, Masao Seto;
Expression of the TCL1 gene at 14q32 in B-cell malignancies but not in adult T-cell leukemia.
Jpn. J. Cancer Res. 89:712-718(1998)

PubMed=10637496; DOI=10.1038/sj.leu.2401604
Hans Gunther Drexler, Severine Fombonne, Yoshinobu Matsuo, Z.-B. Hu, Hiroyuki Hamaguchi, Cord C. Uphoff;
p53 alterations in human leukemia-lymphoma cell lines: in vitro artifact or prerequisite for cell immortalization?
Leukemia 14:198-206(2000)

DOI=10.1016/B978-0-12-221970-2.50457-5
Hans Gunther Drexler;
The leukemia-lymphoma cell line factsbook.
(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001)

PubMed=11753612; DOI=10.1038/sj.leu.2402318
Zhang-Xi Xiao, Melvyn Francis Greaves, Patricia Ann Buffler, Martyn T. Smith, Mark R. Segal, Brian M. Dicks, John K. Wiencke, Joseph Leo Wiemels;
Molecular characterization of genomic AML1-ETO fusions in childhood leukemia.
Leukemia 15:1906-1913(2001)

PubMed=15843827; DOI=10.1038/sj.leu.2403749
Anna K. Hagstrom-Andersson, Patrik Eden, David Lindgren, Jens Nilsson, Carin Lassen, Jesper Heldrup, Magnus Fontes, Ake Borg, Felix Mitelman, Bertil Johansson, Mattias Hoglund, Thoas Fioretos;
Gene expression profiling of leukemic cell lines reveals conserved molecular signatures among subtypes with specific genetic aberrations.
Leukemia 19:1042-1050(2005)

PubMed=18385756; DOI=10.1038/leu.2008.61
Heiko Becker, Dietmar Pfeifer, Jesus Duque-Afonso, Stephen D. Nimer, Hendrik Veelken, Michael Schwabe, Michael Lubbert;
Two cell lines of t(8;21) acute myeloid leukemia with activating KIT exon 17 mutation: models for the 'second hit' hypothesis.
Leukemia 22:1792-1794(2008)

PubMed=25984343; DOI=10.1038/sdata.2014.35; PMCID=PMC4432652
Glenn S. Cowley, Barbara A. Weir, Francisca Vazquez, Pablo Tamayo, Justine A. Scott, Scott Rusin, Alexandra East-Seletsky, Levi D. Ali, William F.J. Gerath, Sarah E. Pantel, Patrick Hall Lizotte ...Show all 40 authors... , Guo-Zhi Jiang, Jessica Hsiao, Aviad Tsherniak, Elizabeth Dwinell, Simon Aoyama, Michael Okamoto, William Harrington, Ellen T. Gelfand, Thomas M. Green, Mark J. Tomko, Shuba Gopal, Terence C. Wong, Hu-Bo Li, Sara Howell, Nicolas Stransky, Ted Liefeld, Dongkeun Jang, Jonathan Bistline, Barbara Hill Meyers, Scott Allen Armstrong, Kenneth Carl Anderson, Kimberly Stegmaier, Michael Reich, David Pellman, Jesse S. Boehm, Jill P. Mesirov, Todd Robert Golub, David E. Root, William Chun Hahn; Show fewer authors
Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.
Sci. Data 1:140035.1-140035.12(2014)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31160637; DOI=10.1038/s41598-019-44491-x; PMCID=PMC6547646
Hilmar Quentmeier, Claudia Pommerenke, Wilhelm Gerhard Dirks, Sonja Eberth, Max Koeppel, Roderick A.F. MacLeod, Stefan Nagel, Klaus G. Steube, Cord C. Uphoff, Hans Gunther Drexler;
The LL-100 panel: 100 cell lines for blood cancer studies.
Sci. Rep. 9:8218.1-8218.14(2019)

PubMed=36982453; DOI=10.3390/ijms24065377; PMCID=PMC10049680
Rafal Skopek, Malgorzata Palusinska, Katarzyna Kaczor-Keller, Rafal Pingwara, Anna Papierniak-Wygladala, Tino Schenk, Slawomir Lewicki, Artur Zelent, Lukasz Szymanski;
Choosing the right cell line for acute myeloid leukemia (AML) research.
Int. J. Mol. Sci. 24:5377.1-5377.34(2023)

Cross-references
Cell line collections (Providers) DSMZ; ACC-690
JCRB; JCRB1170
Cell line databases/resources cancercelllines; CVCL_2196
Cell_Model_Passport; SIDM01864
DepMap; ACH-001656
DSMZCellDive; ACC-690
Biological sample resources BioSample; SAMN03473440
BioSample; SAMN10989585
Encyclopedic resources Wikidata; Q54954758
Gene expression databases ArrayExpress; E-MTAB-7721
ArrayExpress; E-MTAB-7722
GEO; GSM236805
GEO; GSM236841
Polymorphism and mutation databases Cosmic; 787478
Cosmic; 975301
Cosmic; 1149337
Cosmic; 1281353
Cosmic; 1524841
Cosmic; 2306239
Cosmic; 2690678
IARC_TP53; 16154
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number33