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Cellosaurus P815 (CVCL_2154)

[Text version]
Cell line name P815
Synonyms P-815; P 815
Accession CVCL_2154
Resource Identification Initiative To cite this cell line use: P815 (RRID:CVCL_2154)
Comments Part of: Tumor Immunology Bank (TIB) collection from Salk (transferred to ATCC in 1981).
Doubling time: ~18-22 hours (DSMZ=ACC-1).
Transformant: ChEBI; CHEBI_34342; 3-methylcholanthrene (3-MC; 20-methylcholanthrene; 20-MC; MCA).
Omics: Variations; SNP array analysis.
Misspelling: P825; Note=Occasionally.
Sequence variations
  • Mutation; MGI; MGI:96677; Kit; Simple; p.Asp818Tyr (c.2452G>T) (D814Y); Zygosity=Unspecified (PubMed=7513208; PubMed=10210327).
Disease Mouse mast cell neoplasm (NCIt: C21632)
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: DBA/2.
Hierarchy Children:
CVCL_A9JC (P815 Hsp65-IL-2)CVCL_4Z63 (P815 MB)CVCL_4269 (P815-1-1)
CVCL_F735 (P815-X2)CVCL_C0DF (P815.Trh4)
Sex of cell Male
Category Cancer cell line
STR profile Source(s): DSMZ=ACC-1; PubMed=31220119

Markers:
Mouse STR 1-116,17
Mouse STR 1-214,17,18
Mouse STR 2-115
Mouse STR 3-213,14
Mouse STR 4-220.3
Mouse STR 5-513 (PubMed=31220119)
13,14 (DSMZ=ACC-1)
Mouse STR 6-417 (DSMZ=ACC-1)
17,18 (PubMed=31220119)
Mouse STR 6-712 (PubMed=31220119)
12,13 (DSMZ=ACC-1)
Mouse STR 7-127
Mouse STR 8-116
Mouse STR 11-216,17,18
Mouse STR 12-116,17 (DSMZ=ACC-1)
17 (PubMed=31220119)
Mouse STR 13-117,18
Mouse STR 15-320.3,21.3 (PubMed=31220119)
21.3 (DSMZ=ACC-1)
Mouse STR 17-217
Mouse STR 18-317 (PubMed=31220119)
17,18 (DSMZ=ACC-1)
Mouse STR 19-212
Mouse STR X-127 (PubMed=31220119)
27,28 (DSMZ=ACC-1)

Run an STR similarity search on this cell line
Web pages Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/p/cell-lines-detail-232.html
Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/p/cell-lines-detail-233.html
Publications

PubMed=4208429; DOI=10.1038/249049a0
Peter Ralph, Ilona Nakoinz;
Lipopolysaccharides inhibit lymphosarcoma cells of bone marrow orgin.
Nature 249:49-51(1974)

PubMed=1234049; DOI=10.1002/eji.1830050208
Rolf Kiessling, Eva Klein, Hans Wigzell;
'Natural' killer cells in the mouse. I. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Specificity and distribution according to genotype.
Eur. J. Immunol. 5:112-117(1975)

DOI=10.2220/biomedres.5.19
Mariko Yanagisawa, Hiroyuki Hasegawa, Arata Ichiyama, Syun Hosoda, Wataru Nakamura;
Comparison of serotonin-producing murine mastocytomas, P-815 and FMA3: determination of tryptophan hydroxylase, aromatic L-amino acid decarboxylase, and cellular concentration of tryptophan, 5- hydroxytryprophan, 5-hydroxytryptamine and 5-hydroxyindoleacetic acid.
Biomed. Res. 5:19-28(1984)

PubMed=3792703; DOI=10.1111/j.1432-0436.1986.tb00568.x
Pieter J.M. Leenen, Anita M.A.C. Jansen, Willem van Ewijk;
Murine macrophage cell lines can be ordered in a linear differentiation sequence.
Differentiation 32:157-164(1986)

PubMed=7513208; DOI=10.1182/blood.V83.9.2619.2619
Tohru Tsujimura, Takuma Furitsu, Masahiro Morimoto, Koji Isozaki, Shintaro Nomura, Yuji Matsuzawa, Yukihiko Kitamura, Yuzuru Kanakura;
Ligand-independent activation of c-kit receptor tyrosine kinase in a murine mastocytoma cell line P-815 generated by a point mutation.
Blood 83:2619-2626(1994)

PubMed=10210327; DOI=10.1016/S0301-472X(98)00075-7
Cheryl A. London, Stephen Joseph Galli, Toshifumi Yuuki, Zhi-Qing Hu, Stuart C. Helfand, Edwin N. Geissler;
Spontaneous canine mast cell tumors express tandem duplications in the proto-oncogene c-kit.
Exp. Hematol. 27:689-697(1999)

PubMed=18432777; DOI=10.1002/0471142735.im2004s43
Thomas F. Gajewski, Mary A. Markiewicz, Catherine Uyttenhove;
The P815 mastocytoma tumor model.
Curr. Protoc. Immunol. 43:20.4.1-20.4.18(2001)

PubMed=25277546; DOI=10.1186/1471-2164-15-847; PMCID=PMC4198738
John Paul Didion, Ryan J. Buus, Zohreh Naghashfar, David W. Threadgill, Herbert Carpenter Morse 3rd, Fernando Pardo-Manuel de Villena;
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)

PubMed=31220119; DOI=10.1371/journal.pone.0218412; PMCID=PMC6586308
Jamie L. Almeida, Aleksandra Dakic, Karin Kindig, Maikan Kone, Deborah Lee Dormady Letham, Scott Langdon, Ruth Peat, Jayamalini Holding-Pillai, Erin M. Hall, Mark Ladd, Megan D. Shaffer ...Show all 23 authors... , Heath Berg, Jin-Liang Li, Georges Wigger, Steve Lund, Carolyn R. Steffen, Barbara B. Fransway, Bob Geraghty, Manuela Natoli, Beth A. Bauer, Susanne M. Gollin, Dale W. Lewis, Yvonne A. Reid; Show fewer authors
Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.
PLoS ONE 14:e0218412.1-e0218412.24(2019)

Cross-references
Cell line collections (Providers) ATCC; TIB-64
BCRC; 60178
BCRJ; 0200
CCTCC; GDC0608
CLS; 400242 - Discontinued
DSMZ; ACC-1
IZSLER; BS TCL 118
JCRB; IFO50029 - Discontinued
JCRB; JCRB0078
KCB; KCB 93032YJ
NCBI_Iran; C550
RCB; RCB1167
TKG; TKG 0168
Cell line databases/resources CLO; CLO_0008340
CLO; CLO_0008341
CLO; CLO_0050217
CLDB; cl3784
CLDB; cl3785
CLDB; cl5244
CCRID; 1101MOU-PUMC000114
CCRID; 3101MOUSCSP516
CCRID; 3101MOUTCM12
CCRID; 4201MOU-CCTCC00608
DSMZCellDive; ACC-1
Lonza; 187
Anatomy/cell type resources BTO; BTO_0000985
Biological sample resources BioSample; SAMN11397663
Chemistry resources ChEMBL-Cells; CHEMBL3307568
ChEMBL-Targets; CHEMBL614226
PubChem_Cell_line; CVCL_2154
Encyclopedic resources Wikidata; Q54937317
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number34