Cellosaurus cell line HCEC-12 (CVCL

Cellosaurus HCEC-12 (CVCL_2064)

[Text version]

Cell line name

HCEC-12

Synonyms

Human Corneal Endothelial Cells-12

Accession

CVCL_2064

Resource Identification Initiative

To cite this cell line use: HCEC-12 (RRID:CVCL_2064)

Comments

Doubling time: ~40-50 hours (DSMZ=ACC-646).
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pRNS-1).
Derived from site: In situ; Eye, cornea, endothelium; UBERON=UBERON_0001985.
Cell type: Endothelial cell of cornea; CL=CL_0000132.

Species of origin

Homo sapiens (Human) (NCBI Taxonomy: 9606)

Hierarchy

Children:

CVCL_2065 (HCEC-B4G12)

CVCL_2066 (HCEC-H9C1)

Sex of cell

Female

Age at sampling

91Y

Category

Transformed cell line

STR profile

Source(s): DSMZ=ACC-646

Markers:

Amelogenin	X
CSF1PO	12,13
D2S1338	18,25
D3S1358	15,16
D5S818	11,12
D7S820	10,11
D8S1179	13
D13S317	8,11
D16S539	11,14
D18S51	15
D19S433	14,15
D21S11	29
FGA	26
Penta D	11,12
Penta E	10
TH01	9,9.3
TPOX	8
vWA	14,17

Publications

PubMed=10794243; DOI=10.1034/j.1600-0420.2000.078002130.x
Bednarz J., Teifel M., Friedl P., Engelmann K.
Immortalization of human corneal endothelial cells using electroporation protocol optimized for human corneal endothelial and human retinal pigment epithelial cells.
Acta Ophthalmol. Scand. 78:130-136(2000)

PubMed=18196893; DOI=10.1159/000113406
Valtink M., Gruschwitz R., Funk R.H.W., Engelmann K.
Two clonal cell lines of immortalized human corneal endothelial cells show either differentiated or precursor cell characteristics.
Cells Tissues Organs 187:286-294(2008)

PubMed=20974632; DOI=10.1136/bjo.2010.183418
Jackel T., Knels L., Valtink M., Funk R.H.W., Engelmann K.
Serum-free corneal organ culture medium (SFM) but not conventional minimal essential organ culture medium (MEM) protects human corneal endothelial cells from apoptotic and necrotic cell death.
Br. J. Ophthalmol. 95:123-130(2011)

Cross-references

Cell line collections (Providers)

DSMZ; ACC-646

Cell line databases/resources

DSMZCellDive; ACC-646
FCS-free; 208-2-425-3-4-12

Biological sample resources

BioSample; SAMN03473460

Encyclopedic resources

Wikidata; Q54881866

Entry history

Entry creation

04-Apr-2012

Last entry update

10-Sep-2024

Version number