Cellosaurus logo
expasy logo

Cellosaurus HCEC-12 (CVCL_2064)

[Text][XML][JSON]
Cell line name HCEC-12
Synonyms Human Corneal Endothelial Cells-12
Accession CVCL_2064
Resource Identification Initiative To cite this cell line use: HCEC-12 (RRID:CVCL_2064)
Comments Doubling time: ~40-50 hours (DSMZ=ACC-646).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; Simian virus 40 (SV40) large T antigen.
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pRNS-1).
Derived from site: In situ; Eye, cornea, endothelium; UBERON=UBERON_0001985.
Cell type: Endothelial cell of cornea; CL=CL_0000132.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_2065 (HCEC-B4G12)CVCL_2066 (HCEC-H9C1)
Sex of cell Female
Age at sampling 91Y
Category Transformed cell line
STR profile Source(s): DSMZ=ACC-646

Markers:
AmelogeninX
CSF1PO12,13
D2S133818,25
D3S135815,16
D5S81811,12
D7S82010,11
D8S117913
D13S3178,11
D16S53911,14
D18S5115
D19S43314,15
D21S1129
FGA26
Penta D11,12
Penta E10
TH019,9.3
TPOX8
vWA14,17

Run an STR similarity search on this cell line
Publications

PubMed=10794243; DOI=10.1034/j.1600-0420.2000.078002130.x
Jurgen Bednarz, Michael Teifel, Peter Friedl, Katrin Engelmann;
Immortalization of human corneal endothelial cells using electroporation protocol optimized for human corneal endothelial and human retinal pigment epithelial cells.
Acta Ophthalmol. Scand. 78:130-136(2000)

PubMed=18196893; DOI=10.1159/000113406
Monika Valtink, Rita Gruschwitz, Richard H.W. Funk, Katrin Engelmann;
Two clonal cell lines of immortalized human corneal endothelial cells show either differentiated or precursor cell characteristics.
Cells Tissues Organs 187:286-294(2008)

PubMed=20974632; DOI=10.1136/bjo.2010.183418
Thekla Jackel, Lilla Knels, Monika Valtink, Richard H.W. Funk, Katrin Engelmann;
Serum-free corneal organ culture medium (SFM) but not conventional minimal essential organ culture medium (MEM) protects human corneal endothelial cells from apoptotic and necrotic cell death.
Br. J. Ophthalmol. 95:123-130(2011)

Cross-references
Cell line collections (Providers) DSMZ; ACC-646
Cell line databases/resources DSMZCellDive; ACC-646
FCS-free; 208-2-425-3-4-12
Biological sample resources BioSample; SAMN03473460
Encyclopedic resources Wikidata; Q54881866
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number21