ID   HCEC-12
AC   CVCL_2064
SY   Human Corneal Endothelial Cells-12
DR   BioSample; SAMN03473460
DR   DSMZ; ACC-646
DR   DSMZCellDive; ACC-646
DR   FCS-free; 208-2-425-3-4-12
DR   Wikidata; Q54881866
RX   PubMed=10794243;
RX   PubMed=18196893;
RX   PubMed=20974632;
CC   Doubling time: ~40-50 hours (DSMZ=ACC-646).
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P03070; SV40 large T antigen.
CC   Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Transposon Tn5 neo.
CC   Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pRNS-1).
CC   Derived from site: In situ; Eye, cornea, endothelium; UBERON=UBERON_0001985.
CC   Cell type: Endothelial cell of cornea; CL=CL_0000132.
ST   Source(s): DSMZ=ACC-646
ST   Amelogenin: X
ST   CSF1PO: 12,13
ST   D13S317: 8,11
ST   D16S539: 11,14
ST   D18S51: 15
ST   D19S433: 14,15
ST   D21S11: 29
ST   D2S1338: 18,25
ST   D3S1358: 15,16
ST   D5S818: 11,12
ST   D7S820: 10,11
ST   D8S1179: 13
ST   FGA: 26
ST   Penta D: 11,12
ST   Penta E: 10
ST   TH01: 9,9.3
ST   TPOX: 8
ST   vWA: 14,17
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
SX   Female
AG   91Y
CA   Transformed cell line
DT   Created: 04-04-12; Last updated: 10-09-24; Version: 20
//
RX   PubMed=10794243; DOI=10.1034/j.1600-0420.2000.078002130.x;
RA   Bednarz J., Teifel M., Friedl P., Engelmann K.;
RT   "Immortalization of human corneal endothelial cells using
RT   electroporation protocol optimized for human corneal endothelial and
RT   human retinal pigment epithelial cells.";
RL   Acta Ophthalmol. Scand. 78:130-136(2000).
//
RX   PubMed=18196893; DOI=10.1159/000113406;
RA   Valtink M., Gruschwitz R., Funk R.H.W., Engelmann K.;
RT   "Two clonal cell lines of immortalized human corneal endothelial cells
RT   show either differentiated or precursor cell characteristics.";
RL   Cells Tissues Organs 187:286-294(2008).
//
RX   PubMed=20974632; DOI=10.1136/bjo.2010.183418;
RA   Jackel T., Knels L., Valtink M., Funk R.H.W., Engelmann K.;
RT   "Serum-free corneal organ culture medium (SFM) but not conventional
RT   minimal essential organ culture medium (MEM) protects human corneal
RT   endothelial cells from apoptotic and necrotic cell death.";
RL   Br. J. Ophthalmol. 95:123-130(2011).
//