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Cellosaurus MCF7 AREc32 (CVCL_1D32)

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Cell line name MCF7 AREc32
Synonyms MCF7/AREc32; AREc32
Accession CVCL_1D32
Resource Identification Initiative To cite this cell line use: MCF7 AREc32 (RRID:CVCL_1D32)
Comments Population: Caucasian.
Characteristics: Transfected with a construct containing 8 copies of the rat glutathione-S-transferase (GST) antioxidant response element (ARE) linked to the luciferase reporter gene. Induction of the ARE results in luciferase activity (PubMed=17108137).
Genetic integration: Method=Transfection; Gene=UniProtKB; P00552; Klebsiella pneumoniae transposon Tn5 neo.
Genetic integration: Method=Transfection; Gene=UniProtKB; P08659; Photinus pyralis firefly-type luciferase.
Derived from site: Metastatic; Pleural effusion; UBERON=UBERON_0000175.
Sequence variations
  • Gene deletion; HGNC; HGNC:1787; CDKN2A; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:4172; GATA3; Simple; p.Asp336Glyfs*17 (c.1006dupG); Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Glu545Lys (c.1633G>A); ClinVar=VCV000013655; Zygosity=Heterozygous (from parent cell line).
Disease Invasive breast carcinoma of no special type (NCIt: C4194)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0031 (MCF-7)
Sex of cell Female
Age at sampling 69Y
Category Cancer cell line
STR profile Source(s): ECACC=16071902

Markers:
AmelogeninX
CSF1PO10
D3S135816
D5S81811,12
D7S8209
D8S117910,14
D13S31711
D16S53911,12
D18S5114
D21S1130
FGA23,25
Penta D12
Penta E7,12
TH016
TPOX9,12
vWA14,15

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Web pages Provider; CXR Biosciences; -; https://web.archive.org/web/20170818205759/http://www.cxrbiosciences.com/technologies/arec32-cell-line/
Publications

PubMed=17108137; DOI=10.1158/0008-5472.CAN-06-2298
Xiu-Jun Wang, John D. Hayes, Charles Roland Wolf;
Generation of a stable antioxidant response element-driven reporter gene cell line and its use to show redox-dependent activation of nrf2 by cancer chemotherapeutic agents.
Cancer Res. 66:10983-10994(2006)

PubMed=23032559; DOI=10.1039/c2em30506b
Beate Isabella Escher, Mriga Dutt, Erin Maylin, Janet Y.M. Tang, Simon Toze, Charles Roland Wolf, Martin Lang;
Water quality assessment using the AREc32 reporter gene assay indicative of the oxidative stress response pathway.
J. Environ. Monit. 14:2877-2885(2012)

Cross-references
Cell line collections (Providers) ABM; T6103
CancerTools; 151014
ECACC; 16071902 - Discontinued
Ximbio; 151014
Cell line databases/resources cancercelllines; CVCL_1D32
Encyclopedic resources Wikidata; Q54750439
Entry history
Entry creation08-Jul-2015
Last entry update14-Aug-2025
Version number22