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Cellosaurus RTG-2 (CVCL_1920)

[Text version]
Cell line name RTG-2
Synonyms RTG 2; RTG2; Rainbow Trout Gonad-2
Accession CVCL_1920
Resource Identification Initiative To cite this cell line use: RTG-2 (RRID:CVCL_1920)
Comments Group: Fish cell line.
Doubling time: ~2 days (Note=At 20 Celsius) (PubMed=5167740; PubMed=3410806); ~3-7 days (DSMZ=ACC-352).
Anecdotal: First fish cell line to have been established, in 1962.
Misspelling: RTG-22; Note=Occasionally.
Misspelling: RTG22; Note=Occasionally.
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Fibroblast of ovary; CL=CL_2000063.
Species of origin Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri) (NCBI Taxonomy: 8022)
Hierarchy Children:
CVCL_0I50 (RTG-2/f)CVCL_1921 (RTG-P1)
Sex of cell Female
Age at sampling Yearling
Category Spontaneously immortalized cell line
Publications

PubMed=14007940; DOI=10.1126/science.135.3508.1065
Ken Wolf, Millicent Childs Quimby;
Established eurythermic line of fish cells in vitro.
Science 135:1065-1066(1962)

DOI=10.1007/BF02618370
Cyril S. Stulberg, Lewis Lemon Coriell, Alexis John Kniazeff, John E. Shannon;
The animal cell culture collection.
In Vitro 5:1-16(1970)

PubMed=5167740; DOI=10.1007/BF02619003
John A. Plumb, Ken Wolf;
Fish cell growth rates. Quantitative comparison of RTG-2 cell growth at 5-25 degrees C.
In Vitro 7:42-45(1971)

PubMed=235493; DOI=10.1128/IAI.11.4.815-822.1975; PMCID=PMC415140
John De Sena, Guido J. Rio;
Partial purification and characterization of RTG-2 fish cell interferon.
Infect. Immun. 11:815-822(1975)

DOI=10.1139/f78-164
R.K. Kelly, Brian W. Souter, H.R. Miller;
Fish cell lines: comparisons of CHSE-214, FHM, and RTG-2 in assaying IHN and IPN viruses.
J. Fish. Res. Board Can. 35:1009-1011(1978)

DOI=10.3354/dao001209
Takahisa Kimura, Mamoru Yoshimizu, Shigeaki Gorie;
A new rhabdovirus isolated in Japan from cultured hirame (Japanese flounder) Paralichthys olivaceus and ayu Plecoglossus altivelis.
Dis. Aquat. Organ. 1:209-217(1986)

CLPUB00103
Mamoru Yoshimizu, Masanori Kamei, S. Dirakbusarakom, Takahisa Kimura;
Fish cell lines: susceptibility to salmonid viruses.
(In book chapter) Invertebrate and fish tissue culture; Kuroda, Yukiaki & Kurstak, Edouard & Maramorosch, Karl (eds.); pp.207-217; Japan Sci. Soc.; Tokyo; Japan (1988)

PubMed=3410806; DOI=10.1007/BF02623650
Lucy E.J. Lee, A. Martinez, Niels C. Bols;
Culture conditions for arresting and stimulating the proliferation of a rainbow trout fibroblast cell line, RTG-2.
In Vitro Cell. Dev. Biol. 24:795-802(1988)

DOI=10.1111/j.1365-2761.1989.tb01290.x
G. Nicolas Frerichs, Barry J. Hill, Keith Way;
Ulcerative disease rhabdovirus: cell-line susceptibility and serological comparison with other fish rhabdoviruses.
J. Fish Dis. 12:51-56(1989)

PubMed=2591197; DOI=10.1016/0305-0491(89)90357-x
Douglas Redford Tocher, Janette Carr, John R. Sargent;
Polyunsaturated fatty acid metabolism in fish cells: differential metabolism of (n-3) and (n-6) series acids by cultured cells originating from a freshwater teleost fish and from a marine teleost fish.
Comp. Biochem. Physiol. 94B:367-374(1989)

PubMed=1368400; DOI=10.1007/BF02540026
Rosemarie Catherine Ganassin, Niels C. Bols;
Effect of purine supplementation on the growth of salmonid cell lines in different mammalian sera.
Cytotechnology 8:21-29(1992)

DOI=10.3147/jsfp.28.27
Roselyn D. Fernandez, Mamoru Yoshimizu, Yoshio Ezura, Takahisa Kimura;
Comparative growth response of fish cell lines in different media, temperatures, and and sodium chloride concentrations.
Fish Pathol. 28:27-34(1993)

DOI=10.3147/jsfp.30.23
Myung-Joo Oh, Mamoru Yoshimizu, Takahisa Kimura, Yoshio Ezura;
A new virus isolated from salmonid fish.
Fish Pathol. 30:23-32(1995)

PubMed=8736390; DOI=10.1177/019262339602400310
Charleata A. Carter, William W. Ellington, Rebecca J. Van Beneden;
Confocal laser scanning microscopy of oncogene localization in rainbow trout cell lines derived from normal and tumor tissue.
Toxicol. Pathol. 24:339-345(1996)

PubMed=11501281; DOI=10.1002/tox.1039
Sebastian Nehls, Helmut Segner;
Detection of DNA damage in two cell lines from rainbow trout, RTG-2 and RTL-W1, using the comet assay.
Environ. Toxicol. 16:321-329(2001)

PubMed=19002917; DOI=10.1023/A:1020516804173; PMCID=PMC3449788
Guy M.L. Perry, Gordon J. McDonald, Moira M. Ferguson, Rosemarie Catherine Ganassin, Niels C. Bols;
Characterization of rainbow trout cell lines using microsatellite DNA profiling.
Cytotechnology 37:143-151(2001)

DOI=10.1016/S1873-0140(05)80005-0
Niels C. Bols, Vivian R. Dayeh, Lucy E.J. Lee, Kristin Schirmer;
Use of fish cell lines in the toxicology and ecotoxicology of fish Piscine cell lines in environmental toxicology.
(In book chapter) Biochemistry and molecular biology of fishes, Vol. 6; Mommsen, Thomas P. & Moon, Thomas W. (eds.); pp.43-84; Elsevier; Amsterdam; Netherlands (2005)

PubMed=20420918; DOI=10.1016/j.fsi.2010.04.007
Atsushi Kawano, Steve C. Kales, Kazuhiro Fujiki, Stephanie J. DeWitte-Orr, Brian Dixon, Lucy E.J. Lee, Niels C. Bols;
A comparison of rainbow trout cell lines for their expression of the major histocompatibility complex genes and the induction of beta-2-microglobulin by dsRNA.
Fish Shellfish Immunol. 29:312-318(2010)

PubMed=28374170; DOI=10.1007/s11626-017-0142-5
Niels C. Bols, Phuc H. Pham, Vivian R. Dayeh, Lucy E.J. Lee;
Invitromatics, invitrome, and invitroomics: introduction of three new terms for in vitro biology and illustration of their use with the cell lines from rainbow trout.
In Vitro Cell. Dev. Biol. Anim. 53:383-405(2017)

PubMed=33053677; DOI=10.3390/pathogens9100833; PMCID=PMC7601784
Phuc H. Pham, Ehab Misk, Fotini Papazotos, Ginny Jones, Mark P. Polinski, Elena Contador, Spencer Russell, Kyle A. Garver, John S. Lumsden, Niels C. Bols;
Screening of fish cell lines for piscine orthoreovirus-1 (PRV-1) amplification: identification of the non-supportive PRV-1 invitrome.
Pathogens 9:833.1-833.25(2020)

PubMed=34382581; DOI=10.3354/dao03615
Takafumi Ito, Takashi Kamaishi;
Japanese amberjack Seriola quinqueradiata and red sea bream Pagrus major susceptibility to infectious hematopoietic necrosis virus (IHNV) isolate.
Dis. Aquat. Organ. 146:1-8(2021)

Cross-references
Cell line collections (Providers) ATCC; CCL-55
BCRC; 60445
CCLV; CCLV-RIE 0038
CCLV; CCLV-RIE 0088
CCTCC; GDC0169
DSMZ; ACC-352
ECACC; 90102529
IZSLER; BS CL 76
KCB; KCB 89021YJ
Cell line databases/resources CLO; CLO_0008895
CLDB; cl4202
CLDB; cl4203
CLDB; cl4204
DSMZCellDive; ACC-352
Anatomy/cell type resources BTO; BTO_0005472
Encyclopedic resources Wikidata; Q54951397
Entry history
Entry creation04-Apr-2012
Last entry update19-Dec-2024
Version number29