ID   RTG-2
AC   CVCL_1920
SY   RTG 2; RTG2; Rainbow Trout Gonad-2
DR   BTO; BTO:0005472
DR   CLO; CLO_0008895
DR   CLDB; cl4202
DR   CLDB; cl4203
DR   CLDB; cl4204
DR   ATCC; CCL-55
DR   BCRC; 60445
DR   CCLV; CCLV-RIE 0038
DR   CCLV; CCLV-RIE 0088
DR   CCTCC; GDC0169
DR   DSMZ; ACC-352
DR   DSMZCellDive; ACC-352
DR   ECACC; 90102529
DR   IZSLER; BS CL 76
DR   KCB; KCB 89021YJ
DR   Wikidata; Q54951397
RX   CelloPub=CLPUB00103;
RX   DOI=10.1007/BF02618370;
RX   DOI=10.1016/S1873-0140(05)80005-0;
RX   DOI=10.1111/j.1365-2761.1989.tb01290.x;
RX   DOI=10.1139/f78-164;
RX   DOI=10.3147/jsfp.28.27;
RX   DOI=10.3147/jsfp.30.23;
RX   DOI=10.3354/dao001209;
RX   PubMed=235493;
RX   PubMed=1368400;
RX   PubMed=2591197;
RX   PubMed=3410806;
RX   PubMed=5167740;
RX   PubMed=8736390;
RX   PubMed=11501281;
RX   PubMed=14007940;
RX   PubMed=19002917;
RX   PubMed=20420918;
RX   PubMed=28374170;
RX   PubMed=33053677;
RX   PubMed=34382581;
CC   Group: Fish cell line.
CC   Doubling time: ~2 days (Note=At 20 Celsius) (PubMed=5167740; PubMed=3410806); ~3-7 days (DSMZ=ACC-352).
CC   Anecdotal: First fish cell line to have been established, in 1962.
CC   Misspelling: RTG-22; Note=Occasionally.
CC   Misspelling: RTG22; Note=Occasionally.
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Fibroblast of ovary; CL=CL_2000063.
OX   NCBI_TaxID=8022; ! Oncorhynchus mykiss (Rainbow trout)
SX   Female
AG   Yearling
CA   Spontaneously immortalized cell line
DT   Created: 04-04-12; Last updated: 29-06-23; Version: 28
//
RX   CelloPub=CLPUB00103;
RA   Yoshimizu M., Kamei M., Dirakbusarakom S., Kimura T.;
RT   "Fish cell lines: susceptibility to salmonid viruses.";
RL   (In) Invertebrate and fish tissue culture; Kuroda Y., Kurstak E., Maramorosch K. (eds.); pp.207-217; Japan Sci. Soc.; Tokyo (1988).
//
RX   DOI=10.1007/BF02618370;
RA   Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.;
RT   "The animal cell culture collection.";
RL   In Vitro 5:1-16(1970).
//
RX   DOI=10.1016/S1873-0140(05)80005-0;
RA   Bols N.C., Dayeh V.R., Lee L.E.J., Schirmer K.;
RT   "Use of fish cell lines in the toxicology and ecotoxicology of fish.
RT   Piscine cell lines in environmental toxicology.";
RL   (In) Biochemistry and molecular biology of fishes, Vol. 6; Mommsen T.P., Moon T.W. (eds.); pp.43-84; Elsevier; Amsterdam (2005).
//
RX   DOI=10.1111/j.1365-2761.1989.tb01290.x;
RA   Frerichs G.N., Hill B.J., Way K.;
RT   "Ulcerative disease rhabdovirus: cell-line susceptibility and
RT   serological comparison with other fish rhabdoviruses.";
RL   J. Fish Dis. 12:51-56(1989).
//
RX   DOI=10.1139/f78-164;
RA   Kelly R.K., Souter B.W., Miller H.R.;
RT   "Fish cell lines: comparisons of CHSE-214, FHM, and RTG-2 in assaying
RT   IHN and IPN viruses.";
RL   J. Fish. Res. Board Can. 35:1009-1011(1978).
//
RX   DOI=10.3147/jsfp.28.27;
RA   Fernandez R.D., Yoshimizu M., Ezura Y., Kimura T.;
RT   "Comparative growth response of fish cell lines in different media,
RT   temperatures, and and sodium chloride concentrations.";
RL   Fish Pathol. 28:27-34(1993).
//
RX   DOI=10.3147/jsfp.30.23;
RA   Oh M.-J., Yoshimizu M., Kimura T., Ezura Y.;
RT   "A new virus isolated from salmonid fish.";
RL   Fish Pathol. 30:23-32(1995).
//
RX   DOI=10.3354/dao001209;
RA   Kimura T., Yoshimizu M., Gorie S.;
RT   "A new rhabdovirus isolated in Japan from cultured hirame (Japanese
RT   flounder) Paralichthys olivaceus and ayu Plecoglossus altivelis.";
RL   Dis. Aquat. Organ. 1:209-217(1986).
//
RX   PubMed=235493; DOI=10.1128/IAI.11.4.815-822.1975;
RA   De Sena J., Rio G.J.;
RT   "Partial purification and characterization of RTG-2 fish cell
RT   interferon.";
RL   Infect. Immun. 11:815-822(1975).
//
RX   PubMed=1368400; DOI=10.1007/BF02540026;
RA   Ganassin R.C., Bols N.C.;
RT   "Effect of purine supplementation on the growth of salmonid cell lines
RT   in different mammalian sera.";
RL   Cytotechnology 8:21-29(1992).
//
RX   PubMed=2591197; DOI=10.1016/0305-0491(89)90357-x;
RA   Tocher D.R., Carr J., Sargent J.R.;
RT   "Polyunsaturated fatty acid metabolism in fish cells: differential
RT   metabolism of (n-3) and (n-6) series acids by cultured cells
RT   originating from a freshwater teleost fish and from a marine teleost
RT   fish.";
RL   Comp. Biochem. Physiol. 94B:367-374(1989).
//
RX   PubMed=3410806; DOI=10.1007/BF02623650;
RA   Lee L.E.J., Martinez A., Bols N.C.;
RT   "Culture conditions for arresting and stimulating the proliferation of
RT   a rainbow trout fibroblast cell line, RTG-2.";
RL   In Vitro Cell. Dev. Biol. 24:795-802(1988).
//
RX   PubMed=5167740; DOI=10.1007/BF02619003;
RA   Plumb J.A., Wolf K.;
RT   "Fish cell growth rates. Quantitative comparison of RTG-2 cell growth
RT   at 5-25 degrees C.";
RL   In Vitro 7:42-45(1971).
//
RX   PubMed=8736390; DOI=10.1177/019262339602400310;
RA   Carter C.A., Ellington W.W., Van Beneden R.J.;
RT   "Confocal laser scanning microscopy of oncogene localization in
RT   rainbow trout cell lines derived from normal and tumor tissue.";
RL   Toxicol. Pathol. 24:339-345(1996).
//
RX   PubMed=11501281; DOI=10.1002/tox.1039;
RA   Nehls S., Segner H.;
RT   "Detection of DNA damage in two cell lines from rainbow trout, RTG-2
RT   and RTL-W1, using the comet assay.";
RL   Environ. Toxicol. 16:321-329(2001).
//
RX   PubMed=14007940; DOI=10.1126/science.135.3508.1065;
RA   Wolf K., Quimby M.C.;
RT   "Established eurythermic line of fish cells in vitro.";
RL   Science 135:1065-1066(1962).
//
RX   PubMed=19002917; DOI=10.1023/A:1020516804173;
RA   Perry G.M.L., McDonald G.J., Ferguson M.M., Ganassin R.C., Bols N.C.;
RT   "Characterization of rainbow trout cell lines using microsatellite DNA
RT   profiling.";
RL   Cytotechnology 37:143-151(2001).
//
RX   PubMed=20420918; DOI=10.1016/j.fsi.2010.04.007;
RA   Kawano A., Kales S.C., Fujiki K., DeWitte-Orr S.J., Dixon B.,
RA   Lee L.E.J., Bols N.C.;
RT   "A comparison of rainbow trout cell lines for their expression of the
RT   major histocompatibility complex genes and the induction of
RT   beta-2-microglobulin by dsRNA.";
RL   Fish Shellfish Immunol. 29:312-318(2010).
//
RX   PubMed=28374170; DOI=10.1007/s11626-017-0142-5;
RA   Bols N.C., Pham P.H., Dayeh V.R., Lee L.E.J.;
RT   "Invitromatics, invitrome, and invitroomics: introduction of three new
RT   terms for in vitro biology and illustration of their use with the cell
RT   lines from rainbow trout.";
RL   In Vitro Cell. Dev. Biol. Anim. 53:383-405(2017).
//
RX   PubMed=33053677; DOI=10.3390/pathogens9100833;
RA   Pham P.H., Misk E., Papazotos F., Jones G., Polinski M.P.,
RA   Contador E., Russell S., Garver K.A., Lumsden J.S., Bols N.C.;
RT   "Screening of fish cell lines for piscine orthoreovirus-1 (PRV-1)
RT   amplification: identification of the non-supportive PRV-1 invitrome.";
RL   Pathogens 9:833.1-833.25(2020).
//
RX   PubMed=34382581; DOI=10.3354/dao03615;
RA   Ito T., Kamaishi T.;
RT   "Japanese amberjack Seriola quinqueradiata and red sea bream Pagrus
RT   major susceptibility to infectious hematopoietic necrosis virus (IHNV)
RT   isolate.";
RL   Dis. Aquat. Organ. 146:1-8(2021).
//