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Cellosaurus Rh18 (CVCL_1659)

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Cell line name Rh18
Synonyms RH18; RH-18; Rh-18; Rh 18; SJRH-18; SJRH18
Accession CVCL_1659
Secondary accession CVCL_5918
Resource Identification Initiative To cite this cell line use: Rh18 (RRID:CVCL_1659)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: NCI Pediatric Preclinical Testing Program (PPTP) cell line panel.
Population: African American.
Characteristics: Established from a immuno-deficient mice xenograft (HxRh18).
Doubling time: 57.8 hours (PubMed=3607778); 83 hours (PubMed=20922763); ~60-70 hours (DSMZ=ACC-491); 65 hours (COG).
Microsatellite instability: Instable (MSI-low) (Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Quantitative.
Omics: Proteomics; Subcellular; Cell surface.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Perineus, muscle; UBERON=UBERON_0002379.
Sequence variations
Genome ancestry Source: PubMed=30894373

Origin% genome
African74.53
Native American0.16
East Asian, North4.19
East Asian, South0
South Asian0
European, North5.05
European, South16.06
Disease Alveolar rhabdomyosarcoma (NCIt: C3749)
Alveolar rhabdomyosarcoma (ORDO: Orphanet_99756)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_DG02 (Rh18c)
Sex of cell Female
Age at sampling 2Y
Category Cancer cell line
STR profile Source(s): COG; Cosmic-CLP=971774; DSMZ=ACC-491; PubMed=20922763

Markers:
AmelogeninX
CSF1PO10
D2S133819,24
D3S135816
D5S81812
D7S8208,10
D8S117913,15
D13S31712
D16S5399,12
D18S5116,17
D19S43312,14
D21S1132.2,33.2
FGA23
Penta D7,12
Penta E8,13
TH017
TPOX8,9
vWA15,17

Run an STR similarity search on this cell line
Web pages Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/r/cell-lines-detail-557.html
Provider; CCR; -; https://www.cccells.org/PDF_Files/Rhabdomyosarcoma/Rh18%20Cell%20Line%20Data%20Sheet.pdf
Publications

PubMed=6950170; DOI=10.1093/jnci/68.3.437
Janet A. Houghton, Peter J. Houghton, Bruce Leonard Webber;
Growth and characterization of childhood rhabdomyosarcomas as xenografts.
J. Natl. Cancer Inst. 68:437-443(1982)

PubMed=3607778
Bonni J. Hazelton, Janet A. Houghton, David Marion Parham, Edwin C. Douglass, Pamela M. Torrance, Hallie Holt, Peter J. Houghton;
Characterization of cell lines derived from xenografts of childhood rhabdomyosarcoma.
Cancer Res. 47:4501-4507(1987)

CLPUB00569
Paul S. Dickman, Maria G. Tsokos, Timothy J. Triche;
Biology of rhabdomyosarcoma: cell culture, xenografts, and animal models.
(In book chapter) Rhabdomyosarcoma and related tumors in children and adolescents; Maurer, Harold M. & Ruymann, Frederick Beeman & Pochedly, Carl Eugene (eds.); pp.49-88; CRC Press; Boca Raton; USA (1991)

PubMed=8275086; DOI=10.1038/ng1193-230
Naomi Galili, Richard J. Davis, William J. Fredericks, Sunil Mukhopadhyay, Frank J. Rauscher 3rd, Beverly S. Emanuel, Giovanni Rovera, Frederic G. Barr;
Fusion of a fork head domain gene to PAX3 in the solid tumour alveolar rhabdomyosarcoma.
Nat. Genet. 5:230-235(1993)

PubMed=8383879; DOI=10.1126/science.8383879
Stephen Justice Tapscott, Mathew J. Thayer, Harold Martin Weintraub;
Deficiency in rhabdomyosarcomas of a factor required for MyoD activity and myogenesis.
Science 259:1450-1453(1993)

PubMed=7536457; DOI=10.1002/gcc.2870120305
Jaclyn A. Biegel, Lynn M. Nycum, Virginia A. Valentine, Frederic G. Barr, David N. Shapiro;
Detection of the t(2;13)(q35;q14) and PAX3-FKHR fusion in alveolar rhabdomyosarcoma by fluorescence in situ hybridization.
Genes Chromosomes Cancer 12:186-192(1995)

PubMed=9802058
Christina R. McPake, David M. Tillman, Catherine A. Poquette, E. Olusegen George, Janet A. Houghton, Linda C. Harris;
Bax is an important determinant of chemosensitivity in pediatric tumor cell lines independent of Bcl-2 expression and p53 status.
Oncol. Res. 10:235-244(1998)

PubMed=9823299
Javed Khan, Richard M. Simon, Michael Bittner, Yi-Dong Chen, Stephen B. Leighton, Thomas Pohida, Paul D. Smith, Yuan Jiang, Gerald C. Gooden, Jeffrey M. Trent, Paul S. Meltzer;
Gene expression profiling of alveolar rhabdomyosarcoma with cDNA microarrays.
Cancer Res. 58:5009-5013(1998)

PubMed=11051265
Istvan Petak, Leslie Douglas, David M. Tillman, Reka Vernes, Janet A. Houghton;
Pediatric rhabdomyosarcoma cell lines are resistant to Fas-induced apoptosis and highly sensitive to TRAIL-induced apoptosis.
Clin. Cancer Res. 6:4119-4127(2000)

PubMed=19235922; DOI=10.1002/gcc.20655
Edoardo Missiaglia, Joanna Selfe, Mohamed Hamdi, Daniel Williamson, Gerben Schaaf, Cheng Fang, Jan Koster, Brenda M. Summersgill, Boo Messahel, Rogier Versteeg, Kathy Pritchard-Jones ...Show all 13 authors... , Marcel Kool, Janet M. Shipley; Show fewer authors
Genomic imbalances in rhabdomyosarcoma cell lines affect expression of genes frequently altered in primary tumors: an approach to identify candidate genes involved in tumor development.
Genes Chromosomes Cancer 48:455-467(2009)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... , Jonathan Hinton, Ciara Fahey, Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20922763; DOI=10.1002/pbc.22801; PMCID=PMC3005554
Min H. Kang, Malcolm A. Smith, Christopher L. Morton, Nino Keshelava, Peter J. Houghton, Charles Patrick Reynolds;
National Cancer Institute pediatric preclinical testing program: model description for in vitro cytotoxicity testing.
Pediatr. Blood Cancer 56:239-249(2011)

PubMed=23578105; DOI=10.1111/cas.12173; PMCID=PMC7657110
Riki Nishimura, Junko Takita, Aiko Sato-Otsubo, Motohiro Kato, Katsuyoshi Koh, Ryoji Hanada, Yukichi Tanaka, Keisuke Kato, Daichi Maeda, Masashi Fukayama, Masashi Sanada ...Show all 13 authors... , Yasuhide Hayashi, Seishi Ogawa; Show fewer authors
Characterization of genetic lesions in rhabdomyosarcoma using a high-density single nucleotide polymorphism array.
Cancer Sci. 104:856-864(2013)

PubMed=23882450; DOI=10.3389/fonc.2013.00183; PMCID=PMC3713458
Ashley R.P. Hinson, Rosanne Jones, Lisa E.S. Crose, Brian C. Belyea, Frederic G. Barr, Corinne Mary Linardic;
Human rhabdomyosarcoma cell lines for rhabdomyosarcoma research: utility and pitfalls.
Front. Oncol. 3:183.1-183.12(2013)

PubMed=26351324; DOI=10.1158/1535-7163.MCT-15-0074; PMCID=PMC4636476
Beverly A. Teicher, Eric C. Polley, Mark W. Kunkel, David Evans, Thomas E. Silvers, Rene M. Delosh, Julie Laudeman, Chad Ogle, Russell Reinhart, Michael Selby, John Connelly ...Show all 14 authors... , Erik Harris, Anne Monks, Joel Morris; Show fewer authors
Sarcoma cell line screen of oncology drugs and investigational agents identifies patterns associated with gene and microRNA expression.
Mol. Cancer Ther. 14:2452-2462(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=32758582; DOI=10.1016/j.gene.2020.145025
Sai Batchu, Alec Sean Kellish, Abraham A. Hakim;
Assessing alveolar rhabdomyosarcoma cell lines as tumor models by comparison of mRNA expression profiles.
Gene 760:145025.1-145025.5(2020)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

PubMed=36768928; DOI=10.3390/ijms24032601; PMCID=PMC9917031
Andrea Timpanaro, Caroline Piccand, Anne-Christine Uldry, Peter-Karl Bode, Dzhangar Dzhumashev, Rita Sala, Manfred Heller, Jochen Rossler, Michele Bernasconi;
Surfaceome profiling of cell lines and patient-derived xenografts confirm FGFR4, NCAM1, CD276, and highlight AGRL2, JAM3, and L1CAM as surface targets for rhabdomyosarcoma.
Int. J. Mol. Sci. 24:2601.1-2601.28(2023)

Cross-references
Cell line collections (Providers) DSMZ; ACC-491
Cell line databases/resources cancercelllines; CVCL_1659
Cell_Model_Passport; SIDM00454
Cosmic-CLP; 971774
DepMap; ACH-000689
DSMZCellDive; ACC-491
Anatomy/cell type resources BTO; BTO_0005380
Biological sample resources BioSample; SAMN03473565
BioSample; SAMN10989566
Chemistry resources ChEMBL-Cells; CHEMBL3308533
ChEMBL-Targets; CHEMBL2366350
GDSC; 971774
PharmacoDB; RH18_1311_2019
PubChem_Cell_line; CVCL_1659
Encyclopedic resources Wikidata; Q54950333
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM219745
GEO; GSM1670377
GEO; GSM1676325
GEO; GSM1701658
Polymorphism and mutation databases Cosmic; 687954
Cosmic; 801757
Cosmic; 802046
Cosmic; 971774
Cosmic; 1037296
Cosmic; 1048109
Cosmic; 1097747
Cosmic; 1104560
Cosmic; 1108739
Cosmic; 1509196
Cosmic; 1620037
IARC_TP53; 24981
IARC_TP53; 27702
LiGeA; CCLE_325
Proteomic databases PRIDE; PXD030304
PRIDE; PXD039480
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number40