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Cellosaurus NCI-H1993 (CVCL_1512)

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Cell line name NCI-H1993
Synonyms H1993; H-1993; NCIH1993
Accession CVCL_1512
Resource Identification Initiative To cite this cell line use: NCI-H1993 (RRID:CVCL_1512)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: MD Anderson Cell Lines Project.
Population: Caucasian.
Doubling time: 31.6 hours (PubMed=29681454).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Genomics; Whole genome sequencing.
Omics: Phenotyping; CRISPR screening.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Expression; Reverse-phase protein array.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; Array-based CGH.
Omics: Variations; SNP array analysis.
Derived from site: Metastatic; Lymph node; UBERON=UBERON_0000029.
Sequence variations
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Cys242Trp (c.726C>G); ClinVar=VCV000376580; Zygosity=Homozygous (Cosmic-CLP=908476; DepMap=ACH-001137).
Genome ancestry Source: PubMed=30894373

Origin% genome
African1.07
Native American0.66
East Asian, North0.47
East Asian, South1.32
South Asian0.09
European, North67.35
European, South29.05
Disease Lung adenocarcinoma (NCIt: C3512)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Originate from same individual CVCL_E102 ! NCI-BL1993
CVCL_1521 ! NCI-H2073
Sex of cell Female
Age at sampling 47Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-5909; Cosmic-CLP=908476; PubMed=20679594; PubMed=25877200

Markers:
AmelogeninX (ATCC=CRL-5909; Cosmic-CLP=908476; PubMed=25877200)
X,Y (PubMed=20679594)
CSF1PO10,13
D2S133817,23
D3S135817
D5S81811,12
D7S82012
D8S117912,14 (ATCC=CRL-5909)
14 (PubMed=25877200)
D13S31713
D16S53912
D18S5113,18
D19S43313,15
D21S1129
FGA22,23
Penta D10
Penta E12
TH016,9.3
TPOX9
vWA17

Run an STR similarity search on this cell line
Web pages Info; ATCC; -; https://www.atcc.org/en/support/technical-support/faqs/normal-morphology-and-growth-of-atcc-crl-5909-nci-h1993-cells
Info; MCLP; -; https://tcpaportal.org/mclp/
Publications

PubMed=8806092; DOI=10.1002/jcb.240630505
Ruby M. Phelps, Bruce E. Johnson, Daniel C. Ihde, Adi F. Gazdar, David P. Carbone, Patrick R. McClintock, Ritva Ilona Linnoila, Mary J. Matthews, Paul A. Bunn Jr., Desmond Niall Carney, John D. Minna, James L. Mulshine;
NCI-Navy Medical Oncology Branch cell line data base.
J. Cell. Biochem. Suppl. 24:32-91(1996)

PubMed=10987304
Luc Girard, Sabine Zochbauer-Muller, Arvind K. Virmani, Adi F. Gazdar, John D. Minna;
Genome-wide allelotyping of lung cancer identifies new regions of allelic loss, differences between small cell lung cancer and non-small cell lung cancer, and loci clustering.
Cancer Res. 60:4894-4906(2000)

PubMed=11030152; DOI=10.1038/sj.onc.1203815
Sanjay Modi, Akihito Kubo, Herbert Kazuto Oie, Amy B. Coxon, Ahad Rehmatulla, Frederic J. Kaye;
Protein expression of the RB-related gene family and SV40 large T antigen in mesothelioma and lung cancer.
Oncogene 19:4632-4639(2000)

PubMed=16187286; DOI=10.1002/ijc.21491
Cathie Garnis, William W. Lockwood, Emily Vucic, Ying Ge, Luc Girard, John D. Minna, Adi F. Gazdar, Stephen Lam, Calum MacAulay, Wan L. Lam;
High resolution analysis of non-small cell lung cancer cell lines by whole genome tiling path array CGH.
Int. J. Cancer 118:1556-1564(2006)

PubMed=18083107; DOI=10.1016/j.cell.2007.11.025
Klarisa Rikova, Ai-Lan Guo, Qing-Fu Zeng, Anthony Possemato, Jian Yu, Herbert Haack, Julie Nardone, Kimberly Lee, Cynthia Reeves, Yu Li, Ye-Rong Hu ...Show all 30 authors... , Zhi-Ping Tan, Matthew P. Stokes, Laura Sullivan, Jeffrey Mitchell, Randy Wetzel, Joan MacNeill, Jian-Min Ren, Jin Yuan, Corey E. Bakalarski, Judit Villen, Jon M. Kornhauser, Bradley Smith, Dai-Qiang Li, Xin-Min Zhou, Steven P. Gygi, Ting-Lei Gu, Roberto D. Polakiewicz, John Rush, Michael J. Comb; Show fewer authors
Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer.
Cell 131:1190-1203(2007)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... , Jonathan Hinton, Ciara Fahey, Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=20679594; DOI=10.1093/jnci/djq279; PMCID=PMC2935474
Adi F. Gazdar, Luc Girard, William W. Lockwood, Wan L. Lam, John D. Minna;
Lung cancer cell lines as tools for biomedical discovery and research.
J. Natl. Cancer Inst. 102:1310-1321(2010)

PubMed=22961666; DOI=10.1158/2159-8290.CD-12-0112; PMCID=PMC3567922
Lauren Averett Byers, Jing Wang, Monique B. Nilsson, Junya Fujimoto, Pierre Saintigny, John Stuart Yordy, Uma Giri, Michael Peyton, You-Hong Fan, Li-Xia Diao, Fatemeh Masrorpour ...Show all 28 authors... , Li Shen, Wen-Bin Liu, Boris Duchemann, Praveen Tumula, Vikas Bhardwaj, James Welsh, Stephanie Weber, Bonnie S. Glisson, Neda Kalhor, Ignacio Ivan Wistuba, Luc Girard, Scott M. Lippman, Gordon B. Mills, Kevin Robert Coombes, John N. Weinstein, John D. Minna, John Victor Heymach; Show fewer authors
Proteomic profiling identifies dysregulated pathways in small cell lung cancer and novel therapeutic targets including PARP1.
Cancer Discov. 2:798-811(2012)

PubMed=24297535; DOI=10.1142/9789814583220_0008; PMCID=PMC3940063
Oleg Mayba, Florian Gnad, Michael Peyton, Fan Zhang, Kimberly Walter, Pan Du, Melanie A. Huntley, Zhao-Shi Jiang, Jin-Feng Liu, Peter M. Haverty, Robert Clifford Gentleman ...Show all 16 authors... , Rui-Qiang Li, John D. Minna, Ying-Rui Li, David S. Shames, Ze-Min Zhang; Show fewer authors
Integrative analysis of two cell lines derived from a non-small-lung cancer patient -- a panomics approach.
Pac. Symp. Biocomput. 2014:75-86(2014)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005; PMCID=PMC5501076
Jun Li, Wei Zhao, Rehan Akbani, Wen-Bin Liu, Zhen-Lin Ju, Shi-Yun Ling, Christopher P. Vellano, Paul Roebuck, Qing-Hua Yu, A. Karina Eterovic, Lauren Averett Byers ...Show all 25 authors... , Michael A. Davies, Wan-Leng Deng, Y.N. Vashisht Gopal, Guo Chen, Erika Maria von Euw, Dennis Joseph Slamon, Dylan Conklin, John Victor Heymach, Adi F. Gazdar, John D. Minna, Jeffrey N. Myers, Yi-Ling Lu, Gordon B. Mills, Han Liang; Show fewer authors
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

PubMed=29681454; DOI=10.1016/j.cell.2018.03.028; PMCID=PMC5935540
Elizabeth A. McMillan, Myung-Jeom Ryu, Caroline H. Diep, Saurabh Mendiratta, Jean Rene Clemenceau, Rachel M. Vaden, Ju-Hwa Kim, Takashi Motoyaji, Kyle R. Covington, Michael Peyton, Kenneth Huffman ...Show all 40 authors... , Xiao-Feng Wu, Luc Girard, Yeojin Sung, Pei-Hsaun Chen, Prema Latha Mallipeddi, Joo Young Lee, Jordan Hanson, Sukesh Voruganti, Yunku Yu, Sunho Park, Jessica Sudderth, Christopher DeSevo, Donna M. Muzny, HarshaVardhan Doddapaneni, Adi F. Gazdar, Richard Alexander Gibbs, Tae Hyun Hwang, John Victor Heymach, Ignacio Ivan Wistuba, Kevin Robert Coombes, Noelle S. Williams, David A. Wheeler, John B. MacMillan, Ralph J. DeBerardinis, Michael G. Roth, Bruce Arthur Posner, John D. Minna, Hyun Seok Kim, Michael A. White; Show fewer authors
Chemistry-first approach for nomination of personalized treatment in lung cancer.
Cell 173:864-878.e29(2018)

PubMed=30038707; DOI=10.18632/oncotarget.25642; PMCID=PMC6049873
Li-Qin Du, Zhen-Ze Zhao, Milind Bhalchandra Suraokar, Spencer S. Shelton, Xiu-Ye Ma, Tzu-Hung Hsiao, John D. Minna, Ignacio Ivan Wistuba, Alexander Pertsemlidis;
LMO1 functions as an oncogene by regulating TTK expression and correlates with neuroendocrine differentiation of lung cancer.
Oncotarget 9:29601-29618(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Fiona M. Behan, Francesco Iorio, Gabriele Picco, Emanuel Goncalves, Charlotte M. Beaver, Giorgia Migliardi, Rita Santos, Yanhua Rao, Francesco Sassi, Marika Pinnelli, Rizwan Ansari ...Show all 25 authors... , Sarah Harper, David Adam Jackson, Rebecca McRae, Rachel Pooley, Piers Wilkinson, Dieudonne J. van der Meer, David Dow, Carolyn A. Buser-Doepner, Andrea Bertotti, Livio Trusolino, Euan Alexander Stronach, Julio Saez-Rodriguez, Kosuke Yusa, Mathew J. Garnett; Show fewer authors
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)

PubMed=31803961; DOI=10.1002/jcb.29564; PMCID=PMC7496084
James L. Mulshine, Peter Ujhazy, Melissa Antman, Christine M. Burgess, Igor A. Kuzmin, Paul A. Bunn Jr., Bruce E. Johnson, Jack Alan Roth, Harvey I. Pass, Sheila M. Ross, Carolyn R. Aldige ...Show all 13 authors... , Ignacio Ivan Wistuba, John D. Minna; Show fewer authors
From clinical specimens to human cancer preclinical models -- a journey the NCI-cell line database-25 years later.
J. Cell. Biochem. 121:3986-3999(2020)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) ATCC; CRL-5909
NCI-DTP; NCI-H1993
Cell line databases/resources CLO; CLO_0008036
cancercelllines; CVCL_1512
Cell_Model_Passport; SIDM00758
Cosmic-CLP; 908476
DepMap; ACH-001137
IGRhCellID; NCIH1993
LINCS_LDP; LCL-1646
Biological sample resources BioSample; SAMN03473255
CRISP screens repositories BioGRID_ORCS_Cell_line; 959
Chemistry resources ChEMBL-Cells; CHEMBL3308138
ChEMBL-Targets; CHEMBL1075527
GDSC; 908476
PharmacoDB; NCIH1993_1054_2019
PubChem_Cell_line; CVCL_1512
Encyclopedic resources Wikidata; Q54907897
Experimental variables resources EFO; EFO_0002272
Gene expression databases ArrayExpress; E-MTAB-38
ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-3610
GEO; GSM62328
GEO; GSM108831
GEO; GSM108832
GEO; GSM274706
GEO; GSM434315
GEO; GSM513952
GEO; GSM514337
GEO; GSM794341
GEO; GSM817095
GEO; GSM1670204
Polymorphism and mutation databases Cosmic; 687809
Cosmic; 877248
Cosmic; 903608
Cosmic; 908476
Cosmic; 980988
Cosmic; 1032460
Cosmic; 1146895
Cosmic; 1694669
Cosmic; 1870266
Cosmic; 1891898
Cosmic; 1995549
Cosmic; 2125239
Cosmic; 2630415
IARC_TP53; 21550
Progenetix; CVCL_1512
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number46