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Cellosaurus HuO9 (CVCL_1298)

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Cell line name HuO9
Synonyms Huo9; HUO9; Hu09
Accession CVCL_1298
Resource Identification Initiative To cite this cell line use: HuO9 (RRID:CVCL_1298)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: Japanese.
Characteristics: Established from a xenograft established in athymic nude mice after five passages.
Doubling time: 57 hours (PubMed=2170070); 43.3 hours (PubMed=14669798).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; ChIP-seq; H3K27ac.
Omics: Genomics; ChIP-seq; H3K4me1.
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Bone, right distal femur; UBERON=UBERON_0004406.
Sequence variations
HLA typing Source: PubMed=15289353
Class I
HLA-AA*24:01,33:03
HLA-BB*44:03:01,51:01
HLA-CC*14:02,14:03
Genome ancestry Source: PubMed=30894373

Origin% genome
African2.89
Native American0
East Asian, North77.77
East Asian, South13.74
South Asian0
European, North1.78
European, South3.83
Disease Osteosarcoma (NCIt: C9145)
Osteosarcoma (ORDO: Orphanet_668)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_WL11 (HuO9-H3)CVCL_WL12 (HuO9-L12)CVCL_WL13 (HuO9-L13)
CVCL_WL14 (HuO9-L6)CVCL_R725 (HuO9-M112)CVCL_R726 (HuO9-M132)
CVCL_JG37 (HuO9/CMV-Luc)
Originate from same individual CVCL_2958 ! HuO9N2
Sex of cell Female
Age at sampling 13Y
Category Cancer cell line
STR profile Source(s): Cosmic-CLP=907072; DepMap=ACH-001526; JCRB=JCRB0427

Markers:
AmelogeninX
CSF1PO10,11
D3S135816
D5S81813
D7S82010
D8S117915
D13S31711
D16S53911
D18S5113
D21S1128
FGA24
Penta D11,13
Penta E17,21
TH019
TPOX9
vWA14

Run an STR similarity search on this cell line
Publications

PubMed=2691514; DOI=10.1007/BF00391353
Akira Kawai, Toshifumi Ozaki, Shogo Ikeda, Takuzo Oda, Masahiro Miyazaki, Jiro Sato, Kazuhisa Taketa, Hajime Inoue;
Two distinct cell lines derived from a human osteosarcoma.
J. Cancer Res. Clin. Oncol. 115:531-536(1989)

PubMed=2170070; DOI=10.1097/00003086-199010000-00036
Akira Kawai;
A newly established human osteosarcoma cell line with osteoblastic properties.
Clin. Orthop. Relat. Res. 259:256-267(1990)

DOI=10.11418/jtca1981.16.3_173
Koichiro Mihara, Masahiro Miyazaki, Kazuo Fushimi, Toshiya Tsuji, Yusuke Inoue, Ken-ichi Fukaya, Ryuichiro Ohashi, Masayoshi Namba;
The p53 gene status and other cellular characteristics of human cell lines maintained in our laboratory.
Tissue Cult. Res. Commun. 16:173-178(1997)

PubMed=9359923; DOI=10.18926/AMO/30789
Koichiro Mihara, Masahiro Miyazaki, Tadashi Kondo, Kazuo Fushimi, Toshiya Tsuji, Yusuke Inoue, Ken-ichi Fukaya, Chikashi Ishioka, Masayoshi Namba;
Yeast functional assay of the p53 gene status in human cell lines maintained in our laboratory.
Acta Med. Okayama 51:261-265(1997)

PubMed=12405284; DOI=10.1023/A:1020395816633
Kenji Kimura, Tetsuhiro Nakano, Yong-Bum Park, Masachika Tani, Hiroyuki Tsuda, Yasuo Beppu, Hideshige Moriya, Jun Yokota;
Establishment of human osteosarcoma cell lines with high metastatic potential to lungs and their utilities for therapeutic studies on metastatic osteosarcoma.
Clin. Exp. Metastasis 19:477-485(2002)

PubMed=14669798; DOI=10.1023/A:1027355610603
Tetsuhiro Nakano, Masachika Tani, Yasunori Ishibashi, Kenji Kimura, Yong-Bum Park, Natsuko Imaizumi, Hiroyuki Tsuda, Kazuhiko Aoyagi, Hiroki Sasaki, Susumu Ohwada, Jun Yokota;
Biological properties and gene expression associated with metastatic potential of human osteosarcoma.
Clin. Exp. Metastasis 20:665-674(2003)

PubMed=15289353; DOI=10.1158/0008-5472.CAN-04-0522
Tomohide Tsukahara, Yuki Nabeta, Satoshi Kawaguchi, Hideyuki Ikeda, Yuriko Sato, Kumiko Shimozawa, Kazunori Ida, Hiroko Asanuma, Yoshihiko Hirohashi, Toshihiko Torigoe, Hiroaki Hiraga, Satoshi Nagoya, Takuro Wada ...Show all 15 authors... , Toshihiko Yamashita, Noriyuki Sato; Show fewer authors
Identification of human autologous cytotoxic T-lymphocyte-defined osteosarcoma gene that encodes a transcriptional regulator, papillomavirus binding factor.
Cancer Res. 64:5442-5448(2004)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa, Jonathan Hinton, Ciara Fahey ...Show all 22 authors... , Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=26320182; DOI=10.18632/oncotarget.5177; PMCID=PMC4745740
Ling Ren, Arnulfo Mendoza, Jack Zhu, Joseph W. Briggs, Charles Halsey, Ellen S. Hong, Sandra Sczerba Burkett, James J. Morrow, Michael M. Lizardo, Tanasa Osborne, Samuel Q. Li, Hue H. Luu, Paul S. Meltzer ...Show all 14 authors... , Chand Khanna; Show fewer authors
Characterization of the metastatic phenotype of a panel of established osteosarcoma cells.
Oncotarget 6:29469-29481(2015)

PubMed=26351324; DOI=10.1158/1535-7163.MCT-15-0074; PMCID=PMC4636476
Beverly A. Teicher, Eric C. Polley, Mark W. Kunkel, David Evans, Thomas E. Silvers, Rene M. Delosh, Julie Laudeman, Chad Ogle, Russell Reinhart, Michael Selby, John Connelly, Erik Harris, Anne Monks ...Show all 14 authors... , Joel Morris; Show fewer authors
Sarcoma cell line screen of oncology drugs and investigational agents identifies patterns associated with gene and microRNA expression.
Mol. Cancer Ther. 14:2452-2462(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer, Patricia Greninger, Ewald van Dyk ...Show all 39 authors... , Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=29334376; DOI=10.1038/nm.4475; PMCID=PMC5803371
James J. Morrow, Ian Bayles, Alister Peter William Funnell, Tyler Eugene Miller, Alina Saiakhova, Michael M. Lizardo, Cynthia F. Bartels, Maaike Y. Kapteijn, Stevephen Hung, Arnulfo Mendoza, Gursimran Dhillon, Daniel R. Chee, Jay T. Myers ...Show all 26 authors... , Frederick Allen, Marco Gambarotti, Alberto Righi, Analisa DiFeo, Brian P. Rubin, Alex Y. Huang, Paul S. Meltzer, Lee J. Helman, Piero Picci, Hendrik Henri Versteeg, John A. Stamatoyannopoulos, Chand Khanna, Peter C. Scacheri; Show fewer authors
Positively selected enhancer elements endow osteosarcoma cells with metastatic competence.
Nat. Med. 24:176-185(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker, Jennifer Koh, Howard Lightfoot ...Show all 32 authors... , Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) JCRB; JCRB0427
Cell line databases/resources CLO; CLO_0009875
cancercelllines; CVCL_1298
Cell_Model_Passport; SIDM00599
Cosmic-CLP; 907072
DepMap; ACH-001526
LINCS_LDP; LCL-1423
Biological sample resources BioSample; SAMN03471626
Chemistry resources ChEMBL-Cells; CHEMBL3308748
ChEMBL-Targets; CHEMBL1075476
GDSC; 907072
PharmacoDB; HuO9_643_2019
PubChem_Cell_line; CVCL_1298
Encyclopedic resources Wikidata; Q54896887
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-3610
GEO; GSM320823
GEO; GSM1669921
GEO; GSM1676303
GEO; GSM1701638
GEO; GSM1914988
GEO; GSM1914989
GEO; GSM1914990
GEO; GSM1914991
Polymorphism and mutation databases Cosmic; 907072
Cosmic; 931040
IARC_TP53; 21390
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number41