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Cellosaurus CAL-12T (CVCL_1105)

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Cell line name CAL-12T
Synonyms Cal-12T; Cal.12T; CAL 12T; CAL12T; Cal12T; CAL 12; Centre Antoine Lacassagne-12T
Accession CVCL_1105
Resource Identification Initiative To cite this cell line use: CAL-12T (RRID:CVCL_1105)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Population: Caucasian.
Doubling time: 35 hours (PubMed=1657424; PubMed=2145926); ~50 hours (DSMZ=ACC-443).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Lung; UBERON=UBERON_0002048.
Sequence variations
  • Mutation; HGNC; HGNC:18037; ARID2; Simple; p.Gly1291Glufs*4 (c.3872del) (p.Val1290fs, c.3870delG); Zygosity=Heterozygous (Cosmic-CLP=753540; DepMap=ACH-000826).
  • Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Gly466Val (c.1397G>T); ClinVar=VCV000013967; Zygosity=Heterozygous (Cosmic-CLP=753540; DepMap=ACH-000826).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Cys135Phe (c.404G>T); ClinVar=VCV000376559; Zygosity=Homozygous (Cosmic-CLP=753540; DepMap=ACH-000826).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*02:01,02:01
HLA-BB*44:02,44:02
HLA-CC*05:01,05:01
Genome ancestry Source: PubMed=30894373

Origin% genome
African0.12
Native American0.91
East Asian, North2.34
East Asian, South0.23
South Asian0
European, North56.03
European, South40.37
Disease Lung non-small cell carcinoma (NCIt: C2926)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_A4II (CAL-12T KRAS (G12C/+))
Sex of cell Male
Age at sampling Age unspecified
Category Cancer cell line
STR profile Source(s): Cosmic-CLP=753540; DSMZ=ACC-443; PubMed=25877200

Markers:
AmelogeninX
CSF1PO13
D2S133820,23
D3S135817
D5S81812
D7S82010,11
D8S117915
D13S31711
D16S53910
D18S5115
D19S43314
D21S1129,30
FGA23,24
Penta D12,13
Penta E11,13
TH016
TPOX8
vWA16

Run an STR similarity search on this cell line
Publications

PubMed=2440566
Jeannine Gioanni, Michel Samson, Evelyne Zanghellini, Christiane Mazeau, Francette Ettore, Francois Demard, Pierre Chauvel, Huguette Duplay, Maurice Schneider, Jean-Claude Laurent, Claude-Michel Lalanne;
Characterization of a new surface epitope specific for human epithelial cells defined by a monoclonal antibody and application to tumor diagnosis.
Cancer Res. 47:4417-4424(1987)

PubMed=2145926; DOI=10.1016/0277-5379(90)90186-W
Stephane Olivier, Patricia Formento, Jean-Louis Fischel, Marie-Christine Etienne-Grimaldi, Gerard Milano;
Epidermal growth factor receptor expression and suramin cytotoxicity in vitro.
Eur. J. Cancer 26:867-871(1990)

PubMed=1657424; DOI=10.1007/BF00685821
Jean-Louis Fischel, Patricia Formento, Maryse Berlion, Jocelyne Berille, Jeannine Gioanni, Jean-Pierre Bizzari, Gerard Milano;
Sequence-dependent cytotoxic effects of the combination of a new nitrosourea, fotemustine, with 5-fluorouracil plus folinic acid.
Cancer Chemother. Pharmacol. 28:448-454(1991)

PubMed=18083107; DOI=10.1016/j.cell.2007.11.025
Klarisa Rikova, Ai-Lan Guo, Qing-Fu Zeng, Anthony Possemato, Jian Yu, Herbert Haack, Julie Nardone, Kimberly Lee, Cynthia Reeves, Yu Li, Ye-Rong Hu ...Show all 30 authors... , Zhi-Ping Tan, Matthew P. Stokes, Laura Sullivan, Jeffrey Mitchell, Randy Wetzel, Joan MacNeill, Jian-Min Ren, Jin Yuan, Corey E. Bakalarski, Judit Villen, Jon M. Kornhauser, Bradley Smith, Dai-Qiang Li, Xin-Min Zhou, Steven P. Gygi, Ting-Lei Gu, Roberto D. Polakiewicz, John Rush, Michael J. Comb; Show fewer authors
Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer.
Cell 131:1190-1203(2007)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... , Jonathan Hinton, Ciara Fahey, Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Jordi Ginesta Barretina, Giordano Caponigro, Nicolas Stransky, Kavitha Venkatesan, Adam A. Margolin, Sungjoon Kim, Christopher J. Wilson, Joseph Lehar, Gregory V. Kryukov, Dmitriy Sonkin, Anupama Reddy ...Show all 61 authors... , Manway Liu, Lauren Murray, Michael F. Berger, John E. Monahan, Paula Morais, Jodi Meltzer, Adam Korejwa, Judit Jane-Valbuena, Felipa A. Mapa, Joseph Thibault, Eva Bric-Furlong, Pichai Raman, Aaron Shipway, Ingo H. Engels, Jill Cheng, Guo-Ying K. Yu, Jian-Jun Yu, Peter Aspesi Jr., Melanie de Silva, Kalpana Jagtap, Michael D. Jones, Li Wang, Charles Hatton, Emanuele Palescandolo, Supriya Gupta, Scott Mahan, Carrie Sougnez, Robert C. Onofrio, Ted Liefeld, Laura E. MacConaill, Wendy Winckler, Michael Reich, Nan-Xin Li, Jill P. Mesirov, Stacey B. Gabriel, Gad Getz, Kristin Ardlie, Vivien Chan, Vic E. Myer, Barbara L. Weber, Jeff Porter, Markus Warmuth, Peter Finan, Jennifer L. Harris, Matthew Langer Meyerson, Todd Robert Golub, Michael P. Morrissey, William Raj Sellers, Robert Schlegel, Levi Alexander Garraway; Show fewer authors
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=25485619; DOI=10.1038/nbt.3080
Christiaan Klijn, Steffen Durinck, Eric W. Stawiski, Peter M. Haverty, Zhao-Shi Jiang, Han-Bin Liu, Jeremiah Degenhardt, Oleg Mayba, Florian Gnad, Jin-Feng Liu, Gregoire Pau ...Show all 30 authors... , Jens Reeder, Yi Cao, Kiran Mukhyala, Suresh K. Selvaraj, Ma-Mie Yu, Gregory J. Zynda, Matthew J. Brauer, Thomas D. Wu, Robert Clifford Gentleman, Gerard Manning, Robert L. Yauch, Richard Bourgon, David Stokoe, Zora Modrusan, Richard M. Neve, Frederic J. de Sauvage, Jeffrey Settleman, Somasekar Seshagiri, Ze-Min Zhang; Show fewer authors
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Jelle Scholtalbers, Sebastian Boegel, Thomas Bukur, Marius Byl, Sebastian Goerges, Patrick Sorn, Martin Loewer, Ugur Sahin, John C. Castle;
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=29681454; DOI=10.1016/j.cell.2018.03.028; PMCID=PMC5935540
Elizabeth A. McMillan, Myung-Jeom Ryu, Caroline H. Diep, Saurabh Mendiratta, Jean Rene Clemenceau, Rachel M. Vaden, Ju-Hwa Kim, Takashi Motoyaji, Kyle R. Covington, Michael Peyton, Kenneth Huffman ...Show all 40 authors... , Xiao-Feng Wu, Luc Girard, Yeojin Sung, Pei-Hsaun Chen, Prema Latha Mallipeddi, Joo Young Lee, Jordan Hanson, Sukesh Voruganti, Yunku Yu, Sunho Park, Jessica Sudderth, Christopher DeSevo, Donna M. Muzny, HarshaVardhan Doddapaneni, Adi F. Gazdar, Richard Alexander Gibbs, Tae Hyun Hwang, John Victor Heymach, Ignacio Ivan Wistuba, Kevin Robert Coombes, Noelle S. Williams, David A. Wheeler, John B. MacMillan, Ralph J. DeBerardinis, Michael G. Roth, Bruce Arthur Posner, John D. Minna, Hyun Seok Kim, Michael A. White; Show fewer authors
Chemistry-first approach for nomination of personalized treatment in lung cancer.
Cell 173:864-878.e29(2018)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) DSMZ; ACC-443
Cell line databases/resources CLO; CLO_0002180
cancercelllines; CVCL_1105
Cell_Model_Passport; SIDM00939
Cosmic-CLP; 753540
DepMap; ACH-000826
DSMZCellDive; ACC-443
LINCS_LDP; LCL-1603
Biological sample resources BioSample; SAMN03473097
BioSample; SAMN10987846
Chemistry resources ChEMBL-Cells; CHEMBL3308132
ChEMBL-Targets; CHEMBL1075406
GDSC; 753540
PharmacoDB; CAL12T_166_2019
PubChem_Cell_line; CVCL_1105
Encyclopedic resources Wikidata; Q54808385
Experimental variables resources EFO; EFO_0006547
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM784241
GEO; GSM827248
GEO; GSM886905
GEO; GSM887970
GEO; GSM1669646
Polymorphism and mutation databases Cosmic; 753540
Cosmic; 1995356
IARC_TP53; 21209
LiGeA; CCLE_097
Progenetix; CVCL_1105
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000610
EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number39