Cellosaurus cell line MLO-A5 (CVCL

Cellosaurus MLO-A5 (CVCL_0P24)

Cross-references
Cell line name	MLO-A5
Synonyms	MLOA5; Murine Long bone Osteocyte-A5
Accession	CVCL_0P24
Resource Identification Initiative	To cite this cell line use: MLO-A5 (RRID:CVCL_0P24)
Comments	Characteristics: Model for the osteoblast to osteocyte differentiation process, the mineralization process and the effects of mechanical loading on biomineralization (Kerafast=EKC003). Genetic integration: Method=Transgenic mouse; Gene=UniProtKB; P03070; SV40 large T antigen. Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40). Derived from site: In situ; Bone, long bone; UBERON=UBERON_0002495. Cell type: Osteocyte; CL=CL_0000137.
Species of origin	Mus musculus (Mouse) (NCBI Taxonomy: 10090) Breed/subspecies: (C57BL/6J x BALB/cJ) F2 Bgla promoter T-Ag transgenic.
Sex of cell	Sex unspecified
Age at sampling	14D
Category	Transformed cell line
Publications	PubMed=11547831; DOI=10.1359/jbmr.2001.16.9.1622 Kato Y., Boskey A.L., Spevak L., Dallas M., Hori M., Bonewald L.F. Establishment of an osteoid preosteocyte-like cell MLO-A5 that spontaneously mineralizes in culture. J. Bone Miner. Res. 16:1622-1633(2001) PubMed=16738320; DOI=10.1128/mcb.02120-05; PMCID=PMC1489126 Zhang K.-Q., Barragan-Adjemian C., Ye L., Kotha S., Dallas M., Lu Y.-B., Zhao S.-J., Harris M., Harris S.E., Feng J.Q., Bonewald L.F. E11/gp38 selective expression in osteocytes: regulation by mechanical strain and role in dendrite elongation. Mol. Cell. Biol. 26:4539-4552(2006) PubMed=22130923; DOI=10.1007/978-1-61779-415-5_6 Rosser J., Bonewald L.F. Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5. Methods Mol. Biol. 816:67-81(2012)
Cell line collections (Providers)	Kerafast; EKC003
Encyclopedic resources	Wikidata; Q54905977
Entry history
Entry creation	10-Apr-2015
Last entry update	10-Sep-2024
Version number	13