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Cellosaurus RN33B (CVCL_0E00)

[Text version]
Cell line name RN33B
Synonyms Rn33B
Accession CVCL_0E00
Resource Identification Initiative To cite this cell line use: RN33B (RRID:CVCL_0E00)
Comments Characteristics: Profiferates at the permissive temperature (33 Celsius). At the restrictive temperature (38.5 Celsius) ceases mitotic activity and differentiates with phase bright cell bodies and 'neuritic-like' processes.
Doubling time: 48 hours (PubMed=8364724).
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=tsA58).
Derived from site: In situ; Embryonic brain, medullary raphe nucleus; UBERON=UBERON_0002692.
Species of origin Rattus norvegicus (Rat) (NCBI Taxonomy: 10116)
Breed/subspecies: Sprague Dawley; RS=RS_0000681.
Hierarchy Children:
CVCL_C1L0 (Rn33B-A68B2M)CVCL_XF90 (RN33B.LV-AA2)
Sex of cell Sex unspecified
Age at sampling 12-13FD
Category Conditionally immortalized cell line
Publications

PubMed=8364724; DOI=10.1016/0006-8993(93)91111-5
Scott R. Whittemore, Linda A. White;
Target regulation of neuronal differentiation in a temperature-sensitive cell line derived from medullary raphe.
Brain Res. 615:27-40(1993)

PubMed=7905006; DOI=10.1016/0165-5728(94)90189-9
Linda A. White, Robert W. Keane, Scott R. Whittemore;
Differentiation of an immortalized CNS neuronal cell line decreases their susceptibility to cytotoxic T cell lysis in vitro.
J. Neuroimmunol. 49:135-143(1994)

PubMed=8699192; DOI=10.1007/BF02284789
Lamya S. Shihabuddin, Jean-Pierre Brunschwig, Vicky R. Holets Whittemore, Mary Bartlett Bunge, Scott R. Whittemore;
Induction of mature neuronal properties in immortalized neuronal precursor cells following grafting into the neonatal CNS.
J. Neurocytol. 25:101-111(1996)

PubMed=12061867; DOI=10.1006/exnr.2002.7888
Cecilia Lundberg, Ulrica Englund, Didier Trono, Anders Bjorklund, Klas Wictorin;
Differentiation of the RN33B cell line into forebrain projection neurons after transplantation into the neonatal rat brain.
Exp. Neurol. 175:370-387(2002)

PubMed=12112642; DOI=10.1002/jgm.268
Nina Rosenqvist, Caroline Hard af Segerstad, Christofer Samuelsson, Jens Johansen, Cecilia Lundberg;
Activation of silenced transgene expression in neural precursor cell lines by inhibitors of histone deacetylation.
J. Gene Med. 4:248-257(2002)

PubMed=15139013; DOI=10.1002/glia.20033
Anita Blixt Wojciechowski, Ulrica Englund, Cecilia Lundberg, Karin Warfvinge;
Migratory capacity of the cell line RN33B and the host glial cell response after subretinal transplantation to normal adult rats.
Glia 47:58-67(2004)

PubMed=22863662; DOI=10.1111/j.1582-4934.2012.01607.x; PMCID=PMC4118228
Asir Alwin Prem Anand, S. Gowri Sankar, Kokila V. Vani;
Immortalization of neuronal progenitors using SV40 large T antigen and differentiation towards dopaminergic neurons.
J. Cell. Mol. Med. 16:2592-2610(2012)

PubMed=31758567; DOI=10.1111/1348-0421.12761
Erina Minato, Atsushi Kobayashi, Keisuke Aoshima, Hideto Fukushi, Takashi Kimura;
Susceptibility of rat immortalized neuronal cell line Rn33B expressing equine major histocompatibility class 1 to equine herpesvirus-1 infection is differentiation dependent.
Microbiol. Immunol. 64:123-132(2020)

Cross-references
Cell line collections (Providers) ATCC; CRL-2825
Encyclopedic resources Wikidata; Q54950747
Experimental variables resources EFO; EFO_0022816
Entry history
Entry creation10-Apr-2015
Last entry update14-Aug-2025
Version number17