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Cellosaurus SK-N-BE(2) (CVCL_0528)

[Text version]
Cell line name SK-N-BE(2)
Synonyms SK-N-BE2; SK-N-BE-2; SKNBE(2); SKNBE-2; SKNBE2; SK-N-BE; SKNBE
Accession CVCL_0528
Resource Identification Initiative To cite this cell line use: SK-N-BE(2) (RRID:CVCL_0528)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
From: Memorial Sloan Kettering Cancer Center; New York; USA.
Registration: Memorial Sloan Kettering Cancer Center Office of Technology Development; SK1980-530.
Population: Caucasian.
Characteristics: Substrate-adherent type (S-type) (PubMed=15720811).
Doubling time: 27 hours (PubMed=29704); ~36 hours (DSMZ=ACC-632).
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Metabolomics.
Omics: Proteomics; PTM; Phosphorylation; Quantitative.
Omics: Proteomics; PTM; Tyrosine phosphorylation.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; Array-based CGH.
Omics: Variations; SNP array analysis.
Derived from site: Metastatic; Bone marrow; UBERON=UBERON_0002371.
Sequence variations
  • Gene deletion; HGNC; HGNC:11998; TP53; Zygosity=Heterozygous (from child cell line SK-N-BE(2)-C).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Cys135Phe (c.404G>T); ClinVar=VCV000376559; Zygosity=Homozygous (PubMed=11507071; DepMap=ACH-000312).
Genome ancestry Source: PubMed=30894373

Origin% genome
African1.71
Native American0.88
East Asian, North1.41
East Asian, South0
South Asian7.87
European, North24.66
European, South63.46
Disease Neuroblastoma (NCIt: C3270)
Neuroblastoma (ORDO: Orphanet_635)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_B0ZE (Abcam SK-N-BE(2) GRM5 KO)CVCL_0529 (SK-N-BE(2)-C)CVCL_0167 (SK-N-BE(2)-M17)
Originate from same individual CVCL_9898 ! SK-N-BE(1)
Sex of cell Male
Age at sampling 2Y2M
Category Cancer cell line
STR profile Source(s): ATCC=CRL-2271; CCRID=3101HUMTCHu200; COG; DSMZ=ACC-632; PubMed=25877200

Markers:
AmelogeninX (DSMZ=ACC-632)
X,Y (ATCC=CRL-2271; CCRID=3101HUMTCHu200; COG; PubMed=25877200)
CSF1PO10
D2S133817,23
D3S135819
D5S81812
D7S8209,10
D8S117913,14
D13S31711
D16S5399,11
D18S5114 (DSMZ=ACC-632)
14,16 (ATCC=CRL-2271; CCRID=3101HUMTCHu200; PubMed=25877200)
16 (COG)
D19S43312,13
D21S1130,31,32.2 (ATCC=CRL-2271; CCRID=3101HUMTCHu200; PubMed=25877200)
30,32.2 (COG; DSMZ=ACC-632)
FGA22,25
Penta D13,14
Penta E14,18
TH016 (DSMZ=ACC-632)
6,7 (ATCC=CRL-2271; CCRID=3101HUMTCHu200; COG; PubMed=25877200)
TPOX8,11
vWA18

Run an STR similarity search on this cell line
Web pages Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/s/cell-lines-detail-565.html
Provider; CCR; -; https://www.cccells.org/dl/NB_Data_Sheets/SK-N-BE2_Cell_Line_Data_Sheet_COGcell_org.pdf
Provider; MSKCC; -; https://www.mskcc.org/research-advantage/support/technology/tangible-material/sk-n-be-2-human-neuroblastoma-cell-line
Publications

DOI=10.1007/978-1-4757-1647-4_13
June Lee Biedler;
Chromosome abnormalities in human tumor cells in culture.
(In book chapter) Human tumor cells in vitro; Fogh, Jorgen (eds.); pp.359-394; Springer; New York; USA (1975)

PubMed=62055; DOI=10.1093/jnci/57.3.683
June Lee Biedler, Barbara A. Spengler;
A novel chromosome abnormality in human neuroblastoma and antifolate-resistant Chinese hamster cell lives in culture.
J. Natl. Cancer Inst. 57:683-695(1976)

PubMed=29704
June Lee Biedler, Suzanne Roffler-Tarlov, Melitta Schachner, Lewis S. Freedman;
Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones.
Cancer Res. 38:3751-3757(1978)

DOI=10.1016/B978-0-12-008304-6.50015-4
June Lee Biedler, Marian B. Meyers, Barbara A. Spengler;
Homogeneously staining regions and double minute chromosomes, prevalent cytogenetic abnormalities of human neuroblastoma cells.
(In book chapter) Advances in cellular neurobiology, Vol. 4; Fedoroff, Sergey & Hertz, Leif (eds.); pp.267-307; Academic Press; New York; USA (1983)

PubMed=6582512; DOI=10.1073/pnas.81.2.568; PMCID=PMC344720
Michael Jules Mattes, Carlos Cordon-Cardo, John L. Lewis Jr., Lloyd John Old, Kenneth Oliver Lloyd;
Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.
Proc. Natl. Acad. Sci. U.S.A. 81:568-572(1984)

PubMed=2535691
Valentina C. Ciccarone, Barbara A. Spengler, Marian B. Meyers, June Lee Biedler, Robert A. Ross;
Phenotypic diversification in human neuroblastoma cells: expression of distinct neural crest lineages.
Cancer Res. 49:219-225(1989)

DOI=10.1016/B978-0-12-333530-2.50006-X
Mark A. Israel, Carol Jeanne Thiele;
Tumor cell lines of the peripheral nervous system.
(In book chapter) Atlas of human tumor cell lines; Hay, Robert J. & Park, Jae-Gahb & Gazdar, Adi F. (eds.); pp.43-78; Academic Press; New York; USA (1994)

PubMed=7838528
Ngan Ching Cheng, Nadine Van Roy, Alvin Chan, Mabel Beitsma, Andries Westerveld, Frank Speleman, Rogier Versteeg;
Deletion mapping in neuroblastoma cell lines suggests two distinct tumor suppressor genes in the 1p35-36 region, only one of which is associated with N-myc amplification.
Oncogene 10:291-297(1995)

DOI=10.1007/0-306-46872-7_2
Carol Jeanne Thiele;
Neuroblastoma.
(In book chapter) Human cell culture. Vol. 1. Cancer cell lines part 1; Masters, John R.W. & Palsson, Bernhard Orn (eds.); pp.21-53; Kluwer Academic Publishers; New York; USA (1999)

PubMed=11507071
Nino Keshelava, Juan J. Zuo, Ping Chen, Sitara N. Waidyaratne, Marian C. Luna, Charles Joseph Gomer, Timothy J. Triche, Charles Patrick Reynolds;
Loss of p53 function confers high-level multidrug resistance in neuroblastoma cell lines.
Cancer Res. 61:6185-6193(2001)

PubMed=11550280; DOI=10.1002/gcc.1174
Nadine Van Roy, Heidi Van Limbergen, Jo Vandesompele, Mireille Van Gele, Bruce Poppe, Helen R. Salwen, Genevieve Laureys, Nunes Manoel, Anne De Paepe, Frank Speleman;
Combined M-FISH and CGH analysis allows comprehensive description of genetic alterations in neuroblastoma cell lines.
Genes Chromosomes Cancer 32:126-135(2001)

PubMed=15720811; DOI=10.1593/neo.04310; PMCID=PMC1531688
Jeanette D. Walton, David R. Kattan, Sharon K. Thomas, Barbara A. Spengler, Hong-Fen Guo, June Lee Biedler, Nai-Kong V. Cheung, Robert A. Ross;
Characteristics of stem cells from human neuroblastoma cell lines and in tumors.
Neoplasia 6:838-845(2004)

PubMed=15892104; DOI=10.1002/gcc.20198
Yael P. Mosse, Joel Greshock, Adam A. Margolin, Tara Naylor, Kristina A. Cole, Deepa Khazi, George Hii, Cynthia Winter, Syed Shahzad, Muhammad Usman Asziz, Jaclyn A. Biegel ...Show all 13 authors... , Barbara L. Weber, John Matthew Maris; Show fewer authors
High-resolution detection and mapping of genomic DNA alterations in neuroblastoma.
Genes Chromosomes Cancer 43:390-403(2005)

PubMed=16822308; DOI=10.1186/1471-2407-6-177; PMCID=PMC1533846
Vincent Dam, Brian T. Morgan, Pavel Mazanek, Michael D. Hogarty;
Mutations in PIK3CA are infrequent in neuroblastoma.
BMC Cancer 6:177.1-177.10(2006)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=20631050; DOI=10.1101/gr.106252.110; PMCID=PMC2928498
Clelia Tiziana Storlazzi, Angelo Lonoce, Maria C. Guastadisegni, Domenico Trombetta, Pietro D'Addabbo, Giulia Daniele, Alberto L'Abbate, Gemma Macchia, Cecilia Surace, Klaas Kok, Reinhard Ullmann ...Show all 16 authors... , Stefania Purgato, Orazio Palumbo, Massimo Carella, Peter F. Ambros, Mariano Rocchi; Show fewer authors
Gene amplification as double minutes or homogeneously staining regions in solid tumors: origin and structure.
Genome Res. 20:1198-1206(2010)

PubMed=20655465; DOI=10.1016/j.cell.2010.06.004; PMCID=PMC2913027
Michael Holzel, Si-Dong Huang, Jan Koster, Ingrid Ora, Arjan Lakeman, Huib Nicolaas Caron, Wouter Nijkamp, Jing Xie, Tom Callens, Shahab Asgharzadeh, Robert C. Seeger ...Show all 14 authors... , Ludwine M. Messiaen, Rogier Versteeg, Rene Bernards; Show fewer authors
NF1 is a tumor suppressor in neuroblastoma that determines retinoic acid response and disease outcome.
Cell 142:218-229(2010)

PubMed=22213050; DOI=10.1002/ijc.27415; PMCID=PMC3757132
Joanna E. Gawecka, Dirk Geerts, Jan Koster, Maisel J. Caliva, Florian J. Sulzmaier, John Opoku-Ansah, Randal K. Wada, Andre S. Bachmann, Joe W. Ramos;
PEA15 impairs cell migration and correlates with clinical features predicting good prognosis in neuroblastoma.
Int. J. Cancer 131:1556-1568(2012)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Jordi Ginesta Barretina, Giordano Caponigro, Nicolas Stransky, Kavitha Venkatesan, Adam A. Margolin, Sungjoon Kim, Christopher J. Wilson, Joseph Lehar, Gregory V. Kryukov, Dmitriy Sonkin, Anupama Reddy ...Show all 61 authors... , Manway Liu, Lauren Murray, Michael F. Berger, John E. Monahan, Paula Morais, Jodi Meltzer, Adam Korejwa, Judit Jane-Valbuena, Felipa A. Mapa, Joseph Thibault, Eva Bric-Furlong, Pichai Raman, Aaron Shipway, Ingo H. Engels, Jill Cheng, Guo-Ying K. Yu, Jian-Jun Yu, Peter Aspesi Jr., Melanie de Silva, Kalpana Jagtap, Michael D. Jones, Li Wang, Charles Hatton, Emanuele Palescandolo, Supriya Gupta, Scott Mahan, Carrie Sougnez, Robert C. Onofrio, Ted Liefeld, Laura E. MacConaill, Wendy Winckler, Michael Reich, Nan-Xin Li, Jill P. Mesirov, Stacey B. Gabriel, Gad Getz, Kristin Ardlie, Vivien Chan, Vic E. Myer, Barbara L. Weber, Jeff Porter, Markus Warmuth, Peter Finan, Jennifer L. Harris, Matthew Langer Meyerson, Todd Robert Golub, Michael P. Morrissey, William Raj Sellers, Robert Schlegel, Levi Alexander Garraway; Show fewer authors
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=24792489; DOI=10.1007/s11060-014-1456-8
Ahsan S. Farooqi, Rebecca A. Dagg, L. Mi Rim Choi, Jerry W. Shay, Charles Patrick Reynolds, Loretta M.S. Lau;
Alternative lengthening of telomeres in neuroblastoma cell lines is associated with a lack of MYCN genomic amplification and with p53 pathway aberrations.
J. Neurooncol. 119:17-26(2014)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=25884760; DOI=10.1371/journal.pcbi.1004130; PMCID=PMC4401789
Juan Palacios-Moreno, Lauren Foltz, Ai-Lan Guo, Matthew P. Stokes, Emily D. Kuehn, Lynn George, Michael J. Comb, Mark L. Grimes;
Neuroblastoma tyrosine kinase signaling networks involve FYN and LYN in endosomes and lipid rafts.
PLoS Comput. Biol. 11:e1004130.1-e1004130.33(2015)

PubMed=28350380; DOI=10.1038/sdata.2017.33; PMCID=PMC5369315
Jo Lynne Harenza, Maura A. Diamond, Rebecca N. Adams, Michael M. Song, Heather L. Davidson, Lori S. Hart, Maiah H. Dent, Paolo Fortina, Charles Patrick Reynolds, John Matthew Maris;
Transcriptomic profiling of 39 commonly-used neuroblastoma cell lines.
Sci. Data 4:170033.1-170033.8(2017)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

Cross-references
Cell line collections (Providers) Abcam; ab275476 - Discontinued
ATCC; CRL-2271
BCRJ; 0381
CancerTools; 161921
CLS; 305058
DSMZ; ACC-632
ECACC; 95011815
ICLC; HTL96015
KCB; KCB 2011099YJ
Cell line databases/resources CLO; CLO_0009052
MCCL; MCC:0000505
CLDB; cl4332
CLDB; cl4333
cancercelllines; CVCL_0528
CCRID; 3101HUMTCHu200
Cell_Model_Passport; SIDM00894
DepMap; ACH-000312
DSMZCellDive; ACC-632
LINCS_LDP; LCL-1980
Anatomy/cell type resources BTO; BTO_0002696
Biological sample resources BioSample; SAMN03472880
BioSample; SAMN03473451
BioSample; SAMN10988136
CRISP screens repositories BioGRID_ORCS_Cell_line; 375
Chemistry resources PharmacoDB; SKNBE_2_1405_2019
Encyclopedic resources Wikidata; Q54954442
Experimental variables resources EFO; EFO_0022401
Gene expression databases ArrayExpress; E-MTAB-2770
GEO; GSM554389
GEO; GSM563358
GEO; GSM692869
GEO; GSM827352
GEO; GSM887593
GEO; GSM888676
GEO; GSM1366411
GEO; GSM1670450
GEO; GSM2371255
GEO; GSM2394375
Metabolomic databases MetaboLights; MTBLS104
Polymorphism and mutation databases Cosmic; 1019928
Cosmic; 2485941
IARC_TP53; 30154
LiGeA; CCLE_929
Progenetix; CVCL_0528
Proteomic databases PRIDE; PXD002635
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number45