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Cellosaurus SK-N-BE(2) (CVCL_0528)

[Text version]
Cell line name SK-N-BE(2)
Accession CVCL_0528
Resource Identification Initiative To cite this cell line use: SK-N-BE(2) (RRID:CVCL_0528)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
From: Memorial Sloan Kettering Cancer Center; New York; USA.
Registration: Memorial Sloan Kettering Cancer Center Office of Technology Development; SK1980-530.
Population: Caucasian.
Characteristics: Substrate-adherent type (S-type) (PubMed=15720811).
Doubling time: 27 hours (PubMed=29704); ~36 hours (DSMZ=ACC-632).
Omics: Array-based CGH.
Omics: Deep exome analysis.
Omics: Deep quantitative phosphoproteome analysis.
Omics: Deep tyrosine phosphoproteome analysis.
Omics: DNA methylation analysis.
Omics: Metabolome analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Derived from site: Metastatic; Bone marrow; UBERON=UBERON_0002371.
Sequence variations
  • Gene deletion; HGNC; 11998; TP53; Zygosity=Heterozygous (from child cell line SK-N-BE(2)-C).
  • Mutation; HGNC; 11998; TP53; Simple; p.Cys135Phe (c.404G>T); ClinVar=VCV000376559; Zygosity=Homozygous (PubMed=11507071; DepMap=ACH-000312).
Genome ancestry Source: PubMed=30894373

Origin% genome
Native American0.88
East Asian, North1.41
East Asian, South0
South Asian7.87
European, North24.66
European, South63.46
Disease Neuroblastoma (NCIt: C3270)
Neuroblastoma (ORDO: Orphanet_635)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_B0ZE (Abcam SK-N-BE(2) GRM5 KO)CVCL_0529 (SK-N-BE(2)-C)CVCL_0167 (SK-N-BE(2)-M17)
Originate from same individual CVCL_9898 ! SK-N-BE(1)
Sex of cell Male
Age at sampling 2Y2M
Category Cancer cell line
STR profile Source(s): ATCC=CRL-2271; CCRID=3101HUMTCHu200; COG; DSMZ=ACC-632; PubMed=25877200

AmelogeninX (DSMZ=ACC-632)
X,Y (ATCC=CRL-2271; CCRID=3101HUMTCHu200; COG; PubMed=25877200)
D18S5114 (DSMZ=ACC-632)
14,16 (ATCC=CRL-2271; CCRID=3101HUMTCHu200; PubMed=25877200)
16 (COG)
D21S1130,31,32.2 (ATCC=CRL-2271; CCRID=3101HUMTCHu200; PubMed=25877200)
30,32.2 (COG; DSMZ=ACC-632)
Penta D13,14
Penta E14,18
TH016 (DSMZ=ACC-632)
6,7 (ATCC=CRL-2271; CCRID=3101HUMTCHu200; COG; PubMed=25877200)

Run an STR similarity search on this cell line
Web pages http://www.cccells.org/dl/NB_Data_Sheets/SK-N-BE2_Cell_Line_Data_Sheet_COGcell_org.pdf

Biedler J.L.
Chromosome abnormalities in human tumor cells in culture.
(In) Human tumor cells in vitro; Fogh J. (eds.); pp.359-394; Springer; New York (1975)

PubMed=62055; DOI=10.1093/jnci/57.3.683
Biedler J.L., Spengler B.A.
A novel chromosome abnormality in human neuroblastoma and antifolate-resistant Chinese hamster cell lives in culture.
J. Natl. Cancer Inst. 57:683-695(1976)

Biedler J.L., Roffler-Tarlov S., Schachner M., Freedman L.S.
Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones.
Cancer Res. 38:3751-3757(1978)

Biedler J.L., Meyers M.B., Spengler B.A.
Homogeneously staining regions and double minute chromosomes, prevalent cytogenetic abnormalities of human neuroblastoma cells.
(In) Advances in cellular neurobiology, Vol. 4; Fedoroff S., Hertz L. (eds.); pp.267-307; Academic Press; New York (1983)

PubMed=6582512; DOI=10.1073/pnas.81.2.568
Mattes M.J., Cordon-Cardo C., Lewis J.L. Jr., Old L.J., Lloyd K.O.
Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.
Proc. Natl. Acad. Sci. U.S.A. 81:568-572(1984)

Ciccarone V.C., Spengler B.A., Meyers M.B., Biedler J.L., Ross R.A.
Phenotypic diversification in human neuroblastoma cells: expression of distinct neural crest lineages.
Cancer Res. 49:219-225(1989)

Israel M.A., Thiele C.J.
Tumor cell lines of the peripheral nervous system.
(In) Atlas of human tumor cell lines; Hay R.J., Park J.-G., Gazdar A.F. (eds.); pp.43-78; Academic Press; New York (1994)

Cheng N.C., Van Roy N., Chan A., Beitsma M., Westerveld A., Speleman F., Versteeg R.
Deletion mapping in neuroblastoma cell lines suggests two distinct tumor suppressor genes in the 1p35-36 region, only one of which is associated with N-myc amplification.
Oncogene 10:291-297(1995)

Thiele C.J.
(In) Human cell culture. Vol. 1. Cancer cell lines part 1; Masters J.R.W., Palsson B.O. (eds.); pp.21-53; Kluwer Academic Publishers; New York (1999)

Keshelava N., Zuo J.J., Chen P., Waidyaratne S.N., Luna M.C., Gomer C.J., Triche T.J., Reynolds C.P.
Loss of p53 function confers high-level multidrug resistance in neuroblastoma cell lines.
Cancer Res. 61:6185-6193(2001)

PubMed=11550280; DOI=10.1002/gcc.1174
Van Roy N., Van Limbergen H., Vandesompele J., Van Gele M., Poppe B., Salwen H.R., Laureys G., Manoel N., De Paepe A., Speleman F.
Combined M-FISH and CGH analysis allows comprehensive description of genetic alterations in neuroblastoma cell lines.
Genes Chromosomes Cancer 32:126-135(2001)

PubMed=15720811; DOI=10.1593/neo.04310
Walton J.D., Kattan D.R., Thomas S.K., Spengler B.A., Guo H.-F., Biedler J.L., Cheung N.-K.V., Ross R.A.
Characteristics of stem cells from human neuroblastoma cell lines and in tumors.
Neoplasia 6:838-845(2004)

PubMed=15892104; DOI=10.1002/gcc.20198
Mosse Y.P., Greshock J., Margolin A.A., Naylor T., Cole K., Khazi D., Hii G., Winter C., Shahzad S., Asziz M.U., Biegel J.A., Weber B.L., Maris J.M.
High-resolution detection and mapping of genomic DNA alterations in neuroblastoma.
Genes Chromosomes Cancer 43:390-403(2005)

PubMed=16822308; DOI=10.1186/1471-2407-6-177
Dam V., Morgan B.T., Mazanek P., Hogarty M.D.
Mutations in PIK3CA are infrequent in neuroblastoma.
BMC Cancer 6:177.1-177.10(2006)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=20631050; DOI=10.1101/gr.106252.110
Storlazzi C.T., Lonoce A., Guastadisegni M.C., Trombetta D., D'Addabbo P., Daniele G., L'Abbate A., Macchia G., Surace C., Kok K., Ullmann R., Purgato S., Palumbo O., Carella M., Ambros P.F., Rocchi M.
Gene amplification as double minutes or homogeneously staining regions in solid tumors: origin and structure.
Genome Res. 20:1198-1206(2010)

PubMed=20655465; DOI=10.1016/j.cell.2010.06.004
Holzel M., Huang S.-D., Koster J., Ora I., Lakeman A., Caron H.N., Nijkamp W., Xie J., Callens T., Asgharzadeh S., Seeger R.C., Messiaen L.M., Versteeg R., Bernards R.
NF1 is a tumor suppressor in neuroblastoma that determines retinoic acid response and disease outcome.
Cell 142:218-229(2010)

PubMed=22213050; DOI=10.1002/ijc.27415
Gawecka J.E., Geerts D., Koster J., Caliva M.J., Sulzmaier F.J., Opoku-Ansah J., Wada R.K., Bachmann A.S., Ramos J.W.
PEA15 impairs cell migration and correlates with clinical features predicting good prognosis in neuroblastoma.
Int. J. Cancer 131:1556-1568(2012)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=24792489; DOI=10.1007/s11060-014-1456-8
Farooqi A.S., Dagg R.A., Choi L.M.R., Shay J.W., Reynolds C.P., Lau L.M.S.
Alternative lengthening of telomeres in neuroblastoma cell lines is associated with a lack of MYCN genomic amplification and with p53 pathway aberrations.
J. Neurooncol. 119:17-26(2014)

PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=25884760; DOI=10.1371/journal.pcbi.1004130
Palacios-Moreno J., Foltz L., Guo A.-L., Stokes M.P., Kuehn E.D., George L., Comb M.J., Grimes M.L.
Neuroblastoma tyrosine kinase signaling networks involve FYN and LYN in endosomes and lipid rafts.
PLoS Comput. Biol. 11:e1004130.1-e1004130.33(2015)

PubMed=28350380; DOI=10.1038/sdata.2017.33
Harenza J.L., Diamond M.A., Adams R.N., Song M.M., Davidson H.L., Hart L.S., Dent M.H., Fortina P., Reynolds C.P., Maris J.M.
Transcriptomic profiling of 39 commonly-used neuroblastoma cell lines.
Sci. Data 4:170033-170033(2017)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

Cell line collections (Providers) Abcam; ab275476 - Discontinued
ATCC; CRL-2271
BCRJ; 0381
CLS; 305058
ECACC; 95011815
ICLC; HTL96015
KCB; KCB 2011099YJ
Cell line databases/resources CLO; CLO_0009052
MCCL; MCC:0000505
CLDB; cl4332
CLDB; cl4333
cancercelllines; CVCL_0528
Cell_Model_Passport; SIDM00894
DepMap; ACH-000312
DSMZCellDive; ACC-632
Anatomy/cell type resources BTO; BTO:0002696
Biological sample resources BioSample; SAMN03472880
BioSample; SAMN03473451
BioSample; SAMN10988136
CRISP screens repositories BioGRID_ORCS_Cell_line; 375
Chemistry resources PharmacoDB; SKNBE_2_1405_2019
Encyclopedic resources Wikidata; Q54954442
Gene expression databases ArrayExpress; E-MTAB-2770
GEO; GSM554389
GEO; GSM563358
GEO; GSM692869
GEO; GSM827352
GEO; GSM887593
GEO; GSM888676
GEO; GSM1366411
GEO; GSM1670450
GEO; GSM2371255
GEO; GSM2394375
Metabolomic databases MetaboLights; MTBLS104
Polymorphism and mutation databases Cosmic; 1019928
Cosmic; 2485941
IARC_TP53; 30154
LiGeA; CCLE_929
Progenetix; CVCL_0528
Proteomic databases PRIDE; PXD002635
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number42