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Cellosaurus RPMI-8226/MR20 (CVCL_0509)

[Text version]
Cell line name RPMI-8226/MR20
Synonyms RPMI 8226/MR20; RPMI8226/MR20; 8226/MR20; 8226 MR20
Accession CVCL_0509
Resource Identification Initiative To cite this cell line use: RPMI-8226/MR20 (RRID:CVCL_0509)
Comments Population: African American.
Characteristics: Produces IgG lambda (from parent cell line).
Selected for resistance to: ChEBI; CHEBI:50729; Mitoxantrone.
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Cell type: B-cell; CL=CL_0000236.
Sequence variations
  • Gene fusion; HGNC; 5722; IGKJ4 + HGNC; 5783; IGKV2-28; Name(s)=IGKV2-28-IGKJ4 (from parent cell line).
  • Gene fusion; HGNC; 5865; IGLJ3 + HGNC; 5888; IGLV2-14; Name(s)=IGLV2-14-IGLJ3 (from parent cell line).
  • Mutation; HGNC; 3236; EGFR; Simple; p.Thr751Ile (c.2252C>T); ClinVar=VCV000177773; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Ala (c.35G>C); ClinVar=VCV000045122; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Glu285Lys (c.853G>A); ClinVar=VCV000420133; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; 12033; TRAF3; Simple; p.Lys191Leufs*60; Zygosity=Unspecified (from parent cell line).
Disease Plasma cell myeloma (NCIt: C3242)
Multiple myeloma (ORDO: Orphanet_29073)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_4V90 (RPMI-8226/MR4)
Sex of cell Male
Age at sampling 61Y
Category Cancer cell line
Publications

PubMed=10070958
Hazlehurst L.A., Foley N.E., Gleason-Guzman M.C., Hacker M.P., Cress A.E., Greenberger L.M., de Jong M.C., Dalton W.S.
Multiple mechanisms confer drug resistance to mitoxantrone in the human 8226 myeloma cell line.
Cancer Res. 59:1021-1028(1999)

PubMed=10825126
Scheffer G.L., Maliepaard M., Pijnenborg A.C.L.M., van Gastelen M.A., de Jong M.C., Schroeijers A.B., van der Kolk D.M., Allen J.D., Ross D.D., van der Valk P., Dalton W.S., Schellens J.H.M., Scheper R.J.
Breast cancer resistance protein is localized at the plasma membrane in mitoxantrone- and topotecan-resistant cell lines.
Cancer Res. 60:2589-2593(2000)

PubMed=11309308
Maliepaard M., Scheffer G.L., Faneyte I.F., van Gastelen M.A., Pijnenborg A.C.L.M., Schinkel A.H., van de Vijver M.J., Scheper R.J., Schellens J.H.M.
Subcellular localization and distribution of the breast cancer resistance protein transporter in normal human tissues.
Cancer Res. 61:3458-3464(2001)

PubMed=12116365; DOI=10.1002/cyto.10111
Minderman H., Suvannasankha A., O'Loughlin K.L., Scheffer G.L., Scheper R.J., Robey R.W., Baer M.R.
Flow cytometric analysis of breast cancer resistance protein expression and function.
Cytometry 48:59-65(2002)

Cross-references
Cell line databases/resources MCCL; MCC:0000407
cancercelllines; CVCL_0509
Encyclopedic resources Wikidata; Q54951250
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number19