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Cellosaurus RL95-2 (CVCL_0505)

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Cell line name RL95-2
Synonyms RL95_2; RL-95-2; RL-952; RL952; RL95
Accession CVCL_0505
Resource Identification Initiative To cite this cell line use: RL95-2 (RRID:CVCL_0505)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: MD Anderson Cell Lines Project.
Part of: TCGA-110-CL cell line panel.
Population: Caucasian.
Doubling time: 22 hours (PubMed=1541432).
Microsatellite instability: Instable (MSI-high) (PubMed=31068700; Sanger).
Omics: Genomics; Chromatin accessibility; ATAC-seq.
Omics: Genomics; DNA methylation analysis.
Omics: Genomics; Whole exome sequencing.
Omics: Phenotyping; CRISPR screening.
Omics: Phenotyping; Drug screening.
Omics: Proteomics; Expression; Reverse-phase protein array.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Endometrium; UBERON=UBERON_0001295.
Sequence variations
  • Mutation; HGNC; HGNC:11110; ARID1A; Simple; p.Arg693Ter (c.2077C>T); Zygosity=Heterozygous (Cosmic-CLP=930082; DepMap=ACH-000965).
  • Mutation; HGNC; HGNC:5173; HRAS; Simple; p.Gln61His (c.183G>T); ClinVar=VCV000376318; Zygosity=Heterozygous (ATCC=CRL-1671; Cosmic-CLP=930082; DepMap=ACH-000965).
  • Mutation; HGNC; HGNC:7133; KMT2D; Simple; p.Arg1252Ter (c.3754C>T); ClinVar=VCV000167228; Zygosity=Heterozygous (Cosmic-CLP=930082; DepMap=ACH-000965).
  • Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Asn323fs*2 (c.968_969insA); Zygosity=Heterozygous (PubMed=20944090).
  • Mutation; HGNC; HGNC:9588; PTEN; Simple; p.Asn323Metfs*21 (c.968delA); ClinVar=VCV000653412; Zygosity=Heterozygous (PubMed=20944090).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Val73Trpfs*50 (c.216delC) (p.P72fs); ClinVar=VCV000428897; Zygosity=Heterozygous (PubMed=9887230; Cosmic-CLP=930082; DepMap=ACH-000965).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Val218del (c.646_648GTG[2]) (c.652_654delGTG); ClinVar=VCV000634761; Zygosity=Heterozygous (PubMed=9887230; Cosmic-CLP=930082; DepMap=ACH-000965).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*02:10,24:02
HLA-BB*55:01,55:01
HLA-CC*03:03,03:03
Class II
HLA-DQDQA1*01:02,05:02
DQB1*05:03,05:03
HLA-DRDRB1*14:01,14:01
Genome ancestry Source: PubMed=30894373

Origin% genome
African0
Native American0.33
East Asian, North1.49
East Asian, South0
South Asian1.52
European, North68.38
European, South28.28
Disease Endometrial adenosquamous carcinoma (NCIt: C114656)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Female
Age at sampling 65Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-1671; Cosmic-CLP=930082; PubMed=25877200; PubMed=33174010; Technion Genomics Center

Markers:
AmelogeninX
CSF1PO10,11
D1S165613,19.3
D2S44111,14
D2S133822,23
D3S135814,16
D5S81810,11
D7S82010
D8S117910,14
D10S124814,15
D12S39115,21
D13S3178,12
D16S53911,13
D18S5110,14
D19S43315
D21S1128,29
D22S104515
FGA20,22
Penta D9
Penta E5,11
TH019,9.3
TPOX8
vWA16,20

Run an STR similarity search on this cell line
Web pages Info; MCLP; -; https://tcpaportal.org/mclp/
Publications

PubMed=6339371; DOI=10.1007/BF02618053
Dennis L. Way, David S. Grosso, John R. Davis, Earl A. Surwit, C.D. Christian;
Characterization of a new human endometrial carcinoma (RL95-2) established in tissue culture.
In Vitro 19:147-158(1983)

PubMed=2436984; DOI=10.1016/0090-8258(87)90286-1
Joel S. Noumoff, Susan W. Haydock, Rajeev Sachdeva, Susan Heyner, M. Lynn Pritchard;
Characteristics of cell lines derived from normal and malignant endometrial tissue.
Gynecol. Oncol. 27:141-149(1987)

PubMed=1541432; DOI=10.1016/0090-8258(92)90045-K
Virpi Rantanen, Seija E. Grenman, Jarmo Kulmala, Tuula Salmi, Reidar Grenman;
Radiation sensitivity of endometrial carcinoma in vitro.
Gynecol. Oncol. 44:217-222(1992)

PubMed=7798295; DOI=10.1007/BF01194268
Virpi Rantanen, Seija E. Grenman, Jarmo Kulmala, Kalle Alanen, Taina Lakkala, Reidar Grenman;
Sublethal damage repair after fractionated irradiation in endometrial cancer cell lines tested with the 96-well plate clonogenic assay.
J. Cancer Res. Clin. Oncol. 120:712-716(1994)

PubMed=8123477; DOI=10.1038/bjc.1994.87; PMCID=PMC1968845
Virpi Rantanen, Seija E. Grenman, Jarmo Kulmala, Reidar Grenman;
Comparative evaluation of cisplatin and carboplatin sensitivity in endometrial adenocarcinoma cell lines.
Br. J. Cancer 69:482-486(1994)

PubMed=9887230; DOI=10.1006/gyno.1998.5194
Virpi Rantanen, Seija E. Grenman, Kaisa Kurvinen, Sakari H. Hietanen, Misa Raitanen, Stina M. Syrjanen;
p53 mutations and presence of HPV DNA do not correlate with radiosensitivity of gynecological cancer cell lines.
Gynecol. Oncol. 71:352-358(1998)

PubMed=12893190; DOI=10.1016/S0090-8258(03)00335-4
Ryoichi Tanaka, Tsuyoshi Saito, Koji Ashihara, Makoto Nishimura, Hisanobu Mizumoto, Ryuichi Kudo;
Three-dimensional coculture of endometrial cancer cells and fibroblasts in human placenta derived collagen sponges and expression matrix metalloproteinases in these cells.
Gynecol. Oncol. 90:297-304(2003)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... , Jonathan Hinton, Ciara Fahey, Bei-Yuan Fu, Sajani Swamy, Gillian L. Dalgliesh, Bin Tean Teh, Panos Deloukas, Feng-Tang Yang, Peter J. Campbell, P. Andrew Futreal, Michael Rudolf Stratton; Show fewer authors
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=20944090; DOI=10.1126/scitranslmed.3001538
Konstantin J. Dedes, Daniel Wetterskog, Ana M. Mendes-Pereira, Rachael Natrajan, Maryou B. Lambros, Felipe Correa Geyer, Radost Vatcheva, Kay Savage, Alan Mackay, Christopher J. Lord, Alan Ashworth, Jorge Sergio Reis-Filho;
PTEN deficiency in endometrioid endometrial adenocarcinomas predicts sensitivity to PARP inhibitors.
Sci. Transl. Med. 2:53ra75.1-53ra75.8(2010)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Jordi Ginesta Barretina, Giordano Caponigro, Nicolas Stransky, Kavitha Venkatesan, Adam A. Margolin, Sungjoon Kim, Christopher J. Wilson, Joseph Lehar, Gregory V. Kryukov, Dmitriy Sonkin, Anupama Reddy ...Show all 61 authors... , Manway Liu, Lauren Murray, Michael F. Berger, John E. Monahan, Paula Morais, Jodi Meltzer, Adam Korejwa, Judit Jane-Valbuena, Felipa A. Mapa, Joseph Thibault, Eva Bric-Furlong, Pichai Raman, Aaron Shipway, Ingo H. Engels, Jill Cheng, Guo-Ying K. Yu, Jian-Jun Yu, Peter Aspesi Jr., Melanie de Silva, Kalpana Jagtap, Michael D. Jones, Li Wang, Charles Hatton, Emanuele Palescandolo, Supriya Gupta, Scott Mahan, Carrie Sougnez, Robert C. Onofrio, Ted Liefeld, Laura E. MacConaill, Wendy Winckler, Michael Reich, Nan-Xin Li, Jill P. Mesirov, Stacey B. Gabriel, Gad Getz, Kristin Ardlie, Vivien Chan, Vic E. Myer, Barbara L. Weber, Jeff Porter, Markus Warmuth, Peter Finan, Jennifer L. Harris, Matthew Langer Meyerson, Todd Robert Golub, Michael P. Morrissey, William Raj Sellers, Robert Schlegel, Levi Alexander Garraway; Show fewer authors
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=22710073; DOI=10.1016/j.ygyno.2012.06.017; PMCID=PMC3432677
Christopher T. Korch, Monique A. Spillman, Twila A. Jackson, Britta M. Jacobsen, Susan K. Murphy, Bruce Arthur Lessey, Virgil Craig Jordan, Andrew Peter Bradford;
DNA profiling analysis of endometrial and ovarian cell lines reveals misidentification, redundancy and contamination.
Gynecol. Oncol. 127:241-248(2012)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Jelle Scholtalbers, Sebastian Boegel, Thomas Bukur, Marius Byl, Sebastian Goerges, Patrick Sorn, Martin Loewer, Ugur Sahin, John C. Castle;
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017; PMCID=PMC4967469
Francesco Iorio, Theo A. Knijnenburg, Daniel J. Vis, Graham Robert Bignell, Michael Patrick Menden, Michael Schubert, Nanne Aben, Emanuel Goncalves, Syd Barthorpe, Howard Lightfoot, Thomas Cokelaer ...Show all 39 authors... , Patricia Greninger, Ewald van Dyk, Han Chang, Heshani de Silva, Holger Heyn, Xian-Ming Deng, Regina K. Egan, Qing-Song Liu, Tatiana Mironenko, Xeni Mitropoulos, Laura Richardson, Jin-Hua Wang, Ting-Hu Zhang, Sebastian Moran, Sergi Sayols, Maryam Soleimani, David Tamborero, Nuria Lopez-Bigas, Petra Ross-Macdonald, Manel Esteller, Nathanael S. Gray, Daniel Arie Haber, Michael Rudolf Stratton, Cyril Henri Benes, Lodewyk F.A. Wessels, Julio Saez-Rodriguez, Ultan McDermott, Mathew J. Garnett; Show fewer authors
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005; PMCID=PMC5501076
Jun Li, Wei Zhao, Rehan Akbani, Wen-Bin Liu, Zhen-Lin Ju, Shi-Yun Ling, Christopher P. Vellano, Paul Roebuck, Qing-Hua Yu, A. Karina Eterovic, Lauren Averett Byers ...Show all 25 authors... , Michael A. Davies, Wan-Leng Deng, Y.N. Vashisht Gopal, Guo Chen, Erika Maria von Euw, Dennis Joseph Slamon, Dylan Conklin, John Victor Heymach, Adi F. Gazdar, John D. Minna, Jeffrey N. Myers, Yi-Ling Lu, Gordon B. Mills, Han Liang; Show fewer authors
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Fiona M. Behan, Francesco Iorio, Gabriele Picco, Emanuel Goncalves, Charlotte M. Beaver, Giorgia Migliardi, Rita Santos, Yanhua Rao, Francesco Sassi, Marika Pinnelli, Rizwan Ansari ...Show all 25 authors... , Sarah Harper, David Adam Jackson, Rebecca McRae, Rachel Pooley, Piers Wilkinson, Dieudonne J. van der Meer, David Dow, Carolyn A. Buser-Doepner, Andrea Bertotti, Livio Trusolino, Euan Alexander Stronach, Julio Saez-Rodriguez, Kosuke Yusa, Mathew J. Garnett; Show fewer authors
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31395879; DOI=10.1038/s41467-019-11415-2; PMCID=PMC6687785
Katharine Yu, Bin Chen, Dvir Aran, Joe Charalel, Christina Yau, Douglas M. Wolf, Laura J. van 't Veer, Atul Janardhan Butte, Ted Goldstein, Marina Sirota;
Comprehensive transcriptomic analysis of cell lines as models of primary tumors across 22 tumor types.
Nat. Commun. 10:3574.1-3574.11(2019)

PubMed=33174010; DOI=10.3892/ijo.2020.5139; PMCID=PMC8811471
Eric J. Devor, Jesus Gonzalez-Bosquet, Kristina W. Thiel, Kimberly K. Leslie;
Genomic characterization of five commonly used endometrial cancer cell lines.
Int. J. Oncol. 57:1348-1357(2020)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010; PMCID=PMC9387775
Emanuel Goncalves, Rebecca C. Poulos, Zhao-Xiang Cai, Syd Barthorpe, Srikanth S. Manda, Natasha Lucas, Alexandra Beck, Daniel Bucio-Noble, Michael Dausmann, Caitlin Hall, Michael Hecker ...Show all 32 authors... , Jennifer Koh, Howard Lightfoot, Sadia Mahboob, Iman Mali, James Morris, Laura Richardson, Akila J. Seneviratne, Rebecca Shepherd, Erin Sykes, Frances Thomas, Sara Valentini, Steven G. Williams, Yang-Xiu Wu, Dylan Xavier, Karen L. MacKenzie, Peter G. Hains, Brett Tully, Phillip James Robinson, Qing Zhong, Mathew J. Garnett, Roger Robert Reddel; Show fewer authors
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cross-references
Cell line collections (Providers) ATCC; CRL-1671
BCRC; 60103
BCRJ; 0366
CCTCC; GDC0113
CLS; 305062
KCB; KCB 2013033YJ
Cell line databases/resources CLO; CLO_0008830
MCCL; MCC:0000402
cancercelllines; CVCL_0505
CCRID; 3101HUMTCHu198
CCRID; 4201HUM-CCTCC00113
Cell_Model_Passport; SIDM01088
Cosmic-CLP; 930082
DepMap; ACH-000965
IGRhCellID; RL952
LINCS_LDP; LCL-1506
Lonza; 1501
Anatomy/cell type resources BTO; BTO_0004491
Biological sample resources BioSample; SAMN03473219
BioSample; SAMN10987787
CRISP screens repositories BioGRID_ORCS_Cell_line; 999
Chemistry resources ChEMBL-Cells; CHEMBL3308843
ChEMBL-Targets; CHEMBL2366168
GDSC; 930082
PharmacoDB; RL952_1319_2019
PubChem_Cell_line; CVCL_0505
Encyclopedic resources Wikidata; Q54950589
Experimental variables resources EFO; EFO_0002320
Gene expression databases ArrayExpress; E-MTAB-38
ArrayExpress; E-MTAB-2770
ArrayExpress; E-MTAB-3610
GEO; GSM374977
GEO; GSM375436
GEO; GSM844677
GEO; GSM887542
GEO; GSM888625
GEO; GSM1670382
GEO; GSM3161728
GEO; GSM3161729
Polymorphism and mutation databases Cosmic; 713477
Cosmic; 713489
Cosmic; 809132
Cosmic; 846175
Cosmic; 871556
Cosmic; 889117
Cosmic; 930082
Cosmic; 980637
Cosmic; 1007165
Cosmic; 1066226
Cosmic; 1070808
Cosmic; 1102377
Cosmic; 1177623
Cosmic; 1223487
Cosmic; 1576464
Cosmic; 1622899
Cosmic; 1696756
Cosmic; 2030481
Cosmic; 2301551
Cosmic; 2646617
Cosmic; 2702441
IARC_TP53; 1114
LiGeA; CCLE_166
Progenetix; CVCL_0505
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number44