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Cellosaurus P39 (CVCL_0478)

[Text version]
Cell line name P39
Synonyms P39/TSU; P39-TSU; P39 TSU; P39TSU; P-39:TSU; P39/Tsugane; P39/TSUGANE; TSUGANE; Tsugane
Accession CVCL_0478
Resource Identification Initiative To cite this cell line use: P39 (RRID:CVCL_0478)
Comments Problematic cell line: Contaminated. Shown to be a HL-60 derivative (PubMed=12592342; PubMed=20143388; PubMed=20421273). Originally thought to originate from the peripheral blood of a 69 year old male with acute myeloid leukemia.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00255.
Population: Caucasian.
Microsatellite instability: Stable (MSS) (PubMed=10739008; PubMed=11226526).
Derived from site: In situ; Peripheral blood; UBERON=UBERON_0000178.
Sequence variations
  • Gene deletion; HGNC; HGNC:11998; TP53; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1787; CDKN2A; Simple; p.Arg80Ter (c.238C>T) (p.Pro94Leu, c.281C>T); ClinVar=VCV000009409; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:7989; NRAS; Simple; p.Gln61Leu (c.182A>T); ClinVar=VCV000375874; Zygosity=Heterozygous (PubMed=9379676).
Disease Adult acute myeloid leukemia with maturation (NCIt: C7961)
Acute myeloblastic leukemia with maturation (ORDO: Orphanet_98834)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0002 (HL-60)
Sex of cell Female
Age at sampling 36Y
Category Cancer cell line
STR profile Source(s): JCRB=JCRB0092

Markers:
AmelogeninX
CSF1PO13,14
D5S81811,12
D7S82011,12
D13S3178,11
D16S53911
TH017,8
TPOX8,11
vWA15,16

Run an STR similarity search on this cell line
Web pages Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Info; JCRB; -; https://cellbank.nibiohn.go.jp/legacy/cellbank/qualitycontrol/identification/summary.htm
Publications

PubMed=6597119; DOI=10.20772/cancersci1959.75.12_1100
Masami Nagai, Shigeki Seki, Takeshi Kitahara, Tatsuo Abe, Keisuke Minato, Shaw Watanabe, Masanori Shimoyama;
A novel human myelomonocytoid cell line, P39/Tsugane, derived from overt leukemia following myelodysplastic syndrome.
Gann 75:1100-1107(1984)

PubMed=9379676; DOI=10.1016/S0145-2126(97)00036-2
Xiao-Ming Sheng, Machiko Kawamura, Hiroaki Ohnishi, Kohmei Ida, Ryoji Hanada, Seiji Kojima, Miyuki Kobayashi, Fumio Bessho, Masayoshi Yanagisawa, Yasuhide Hayashi;
Mutations of the RAS genes in childhood acute myeloid leukemia, myelodysplastic syndrome and juvenile chronic myelocytic leukemia.
Leuk. Res. 21:697-701(1997)

PubMed=10739008; DOI=10.1016/S0145-2126(99)00182-4
Kaoru Inoue, Takashi Kohno, Satoshi Takakura, Yasuhide Hayashi, Hideaki Mizoguchi, Jun Yokota;
Frequent microsatellite instability and BAX mutations in T cell acute lymphoblastic leukemia cell lines.
Leuk. Res. 24:255-262(2000)

DOI=10.1016/B978-0-12-221970-2.50457-5
Hans Gunther Drexler;
The leukemia-lymphoma cell line factsbook.
(In book) ISBN 9780122219702; pp.1-733; Academic Press; London; United Kingdom (2001)

PubMed=11226526; DOI=10.1016/S0145-2126(00)00121-1
Kaoru Inoue, Takashi Kohno, Satoshi Takakura, Yasuhide Hayashi, Hideaki Mizoguchi, Jun Yokota;
Corrigendum to: Frequent microsatellite instability and BAX mutations in T cell acute lymphoblastic leukemia cell lines Leukemia Research 24 (2000), 255-262.
Leuk. Res. 25:275-278(2001)

PubMed=12592342; DOI=10.1038/sj.leu.2402799
Hans Gunther Drexler, Wilhelm Gerhard Dirks, Yoshinobu Matsuo, Roderick A.F. MacLeod;
False leukemia-lymphoma cell lines: an update on over 500 cell lines.
Leukemia 17:416-426(2003)

PubMed=20143388; DOI=10.1002/ijc.25242
Amanda Capes-Davis, George Theodosopoulos, Isobel Atkin, Hans Gunther Drexler, Arihiro Kohara, Roderick A.F. MacLeod, John R.W. Masters, Yukio Nakamura, Yvonne A. Reid, Roger Robert Reddel, Robert Ian Freshney;
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=20421273; DOI=10.3324/haematol.2010.022988; PMCID=PMC2895053
David P. Steensma;
P39/Tsugane cells are a false cell line contaminated with HL-60 cells and are not suitable for mechanistic studies in myelodysplastic syndromes.
Haematologica 95:1229-1230(2010)

PubMed=23955599; DOI=10.1038/ng.2731
Ayana Kon, Lee-Yung Shih, Masashi Minamino, Masashi Sanada, Yuichi Shiraishi, Yasunobu Nagata, Ken-ichi Yoshida, Yusuke Okuno, Masashige Bando, Ryuichiro Nakato, Shumpei Ishikawa ...Show all 41 authors... , Aiko Sato-Otsubo, Genta Nagae, Aiko Nishimoto, Claudia Haferlach, Daniel Nowak, Yusuke Sato, Tamara Alpermann, Masao Nagasaki, Teppei Shimamura, Hiroko Tanaka, Kenichi Chiba, Ryo Yamamoto, Tomoyuki Yamaguchi, Makoto Otsu, Naoshi Obara, Mamiko Sakata-Yanagimoto, Tsuyoshi Nakamaki, Ken Ishiyama, Florian Nolte, Wolf-Karsten Hofmann, Shuichi Miyawaki, Shigeru Chiba, Hiraku Mori, Hiromitsu Nakauchi, Harold Phillip Koeffler, Hiroyuki Aburatani, Torsten Haferlach, Katsuhiko Shirahige, Satoru Miyano, Seishi Ogawa; Show fewer authors
Recurrent mutations in multiple components of the cohesin complex in myeloid neoplasms.
Nat. Genet. 45:1232-1237(2013)

Cross-references
Cell line collections (Providers) JCRB; JCRB0092
Cell line databases/resources MCCL; MCC:0000376
cancercelllines; CVCL_0478
Biological sample resources BioSample; SAMN03151775
BioSample; SAMN03471068
Encyclopedic resources Wikidata; Q54937278
Polymorphism and mutation databases Cosmic; 717681
Cosmic; 947362
Cosmic; 998740
Cosmic; 2089663
IARC_TP53; 21275
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number36