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Cellosaurus NPA (CVCL_0467)

[Text version]
Cell line name NPA
Synonyms NPA-87; NPA'87; NPA 87; NPA87; NPA87-1; UCLA NPA-87-1; UCLA NPA871
Accession CVCL_0467
Secondary accession CVCL_6475
Resource Identification Initiative To cite this cell line use: NPA (RRID:CVCL_0467)
Comments Problematic cell line: Contaminated. Shown to be a M14 derivative (PubMed=18713817; PubMed=20143388; PubMed=21868764). Originally thought to originate from a thyroid gland papillary carcinoma.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00246.
Population: Caucasian.
Derived from site: Metastatic; Right buttock, hypodermis; UBERON=UBERON_0013691+UBERON_0002072.
Sequence variations
  • Mutation; HGNC; 1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; c.150+2T>C (IVS1+2T>C); ClinVar=VCV000406712; Zygosity=Heterozygous; Note=Splice donor mutation (from parent cell line).
  • Mutation; HGNC; 1787; CDKN2A; Simple; c.455insCdel26; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; 11998; TP53; Simple; p.Gly266Glu (c.797G>A); ClinVar=VCV000161516; Zygosity=Heterozygous (PubMed=8423216; PubMed=14522906).
Disease Amelanotic melanoma (NCIt: C3802)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_1395 (M14)
Sex of cell Male
Age at sampling 33Y
Category Cancer cell line
STR profile Source(s): PubMed=18713817; PubMed=21868764


Run an STR similarity search on this cell line
Web pages https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx

PubMed=2551628; DOI=10.1210/endo-125-4-1783
Pang X.-P., Hershman J.M., Chung M., Pekary A.E.
Characterization of tumor necrosis factor-alpha receptors in human and rat thyroid cells and regulation of the receptors by thyrotropin.
Endocrinology 125:1783-1788(1989)

PubMed=8423216; DOI=10.1172/JCI116168
Fagin J.A., Matsuo K., Karmakar A., Chen D.-L., Tang S.-H., Koeffler H.P.
High prevalence of mutations of the p53 gene in poorly differentiated human thyroid carcinomas.
J. Clin. Invest. 91:179-184(1993)

PubMed=8871538; DOI=10.1007/BF00128960
Boghaert E.R., Ain K.B., Taylor K.D., Greenberg V.L., Fowler C., Zimmer S.G.
Quantitative and qualitative differences in growth, invasion and lung colonization of an anaplastic and a papillary human thyroid cancer cell line in vitro and in vivo.
Clin. Exp. Metastasis 14:440-450(1996)

PubMed=11439348; DOI=10.1038/sj.onc.1204531
Frasca F., Vigneri P., Vella V., Vigneri R., Wang J.Y.-J.
Tyrosine kinase inhibitor STI571 enhances thyroid cancer cell motile response to hepatocyte growth factor.
Oncogene 20:3845-3856(2001)

Frasca F., Vella V., Aloisi A., Mandarino A., Mazzon E., Vigneri R., Vigneri P.
p73 tumor-suppressor activity is impaired in human thyroid cancer.
Cancer Res. 63:5829-5837(2003)

PubMed=15980887; DOI=10.1038/labinvest.3700306
Nakamura N., Carney J.A., Jin L., Kajita S., Pallares J., Zhang H.-Y., Qian X., Sebo T.J., Erickson L.A., Lloyd R.V.
RASSF1A and NORE1A methylation and BRAFV600E mutations in thyroid tumors.
Lab. Invest. 85:1065-1075(2005)

PubMed=18713817; DOI=10.1210/jc.2008-1102
Schweppe R.E., Klopper J.P., Korch C.T., Pugazhenthi U., Benezra M., Knauf J.A., Fagin J.A., Marlow L.A., Copland J.A. III, Smallridge R.C., Haugen B.R.
Deoxyribonucleic acid profiling analysis of 40 human thyroid cancer cell lines reveals cross-contamination resulting in cell line redundancy and misidentification.
J. Clin. Endocrinol. Metab. 93:4331-4341(2008)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=21868764; DOI=10.1158/1078-0432.CCR-11-0690
Zhao M., Sano D., Pickering C.R., Jasser S.A., Henderson Y.C., Clayman G.L., Sturgis E.M., Ow T.J., Lotan R., Carey T.E., Sacks P.G., Grandis J.R., Sidransky D., Heldin N.-E., Myers J.N.
Assembly and initial characterization of a panel of 85 genomically validated cell lines from diverse head and neck tumor sites.
Clin. Cancer Res. 17:7248-7264(2011)

Cell line databases/resources MCCL; MCC:0000365
cancercelllines; CVCL_0467
TOKU-E; 4068
Anatomy/cell type resources BTO; BTO:0004771
Biological sample resources BioSample; SAMN03151835
Encyclopedic resources Wikidata; Q54930968
Polymorphism and mutation databases Cosmic; 685605
Cosmic; 686727
Cosmic; 849968
Cosmic; 886547
Cosmic; 918183
Cosmic; 928835
Cosmic; 931239
Cosmic; 990533
Cosmic; 1005456
Cosmic; 1021384
Cosmic; 1071908
Cosmic; 1094772
Cosmic; 1132596
Cosmic; 1152122
Cosmic; 1155264
Cosmic; 1155328
Cosmic; 1161613
Cosmic; 1162848
Cosmic; 1176608
Cosmic; 1699416
IARC_TP53; 2129
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number31