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Cellosaurus NCTC 2544 (CVCL_0461)

[Text version]
Cell line name NCTC 2544
Synonyms NCTC-2544; NCTC2544; NCTC strain 2544
Accession CVCL_0461
Resource Identification Initiative To cite this cell line use: NCTC 2544 (RRID:CVCL_0461)
Comments Problematic cell line: Contaminated. Shown to be a HeLa derivative (PubMed=4864103; PubMed=5641128; PubMed=566722; PubMed=1246601; PubMed=20143388). Originally thought to be a normal skin epithelium cell line.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00012.
Population: African American.
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related cervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0030 (HeLa)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
Web pages https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx

Gartler S.M.
Genetic markers as tracers in cell culture.
Natl. Cancer Inst. Monogr. 26:167-195(1967)

PubMed=5641128; DOI=10.1038/217750a0
Gartler S.M.
Apparent Hela cell contamination of human heteroploid cell lines.
Nature 217:750-751(1968)

PubMed=5668122; DOI=10.3181/00379727-128-33119
Peterson W.D. Jr., Stulberg C.S., Swanborg N.K., Robinson A.R.
Glucose-6-phosphate dehydrogenase isoenzymes in human cell cultures determined by sucrose-agar gel and cellulose acetate zymograms.
Proc. Soc. Exp. Biol. Med. 128:772-776(1968)

Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.
The animal cell culture collection.
In Vitro 5:1-16(1970)

PubMed=990102; DOI=10.1007/BF00572490
Neufahrt A., Forster F.J., Heine H., Schaeg G., Leonhardi G.
Long-term tissue culture of epithelial-like cells from human skin (NCTC strain 2544). I. Measurement of viscosity.
Arch. Dermatol. Res. 256:255-260(1976)

PubMed=1246601; DOI=10.1126/science.1246601
Nelson-Rees W.A., Flandermeyer R.R.
HeLa cultures defined.
Science 191:96-98(1976)

PubMed=565190; DOI=10.1007/BF00455372
Neufahrt A., Forster F.J., Gottschalk K., Leonhardi G.
Long-term tissue culture of epithelial-like cells from human skin (NCTC strain 2544). II. Viscosity changes after enzyme treatment.
Arch. Dermatol. Res. 261:27-31(1978)

PubMed=566722; DOI=10.1007/BF02616110
Lavappa K.S.
Survey of ATCC stocks of human cell lines for HeLa contamination.
In Vitro 14:469-475(1978)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

Cell line collections (Providers) ATCC; CCL-19 - Discontinued
ICLC; HL97002 - Discontinued
Cell line databases/resources CLO; CLO_0008132
MCCL; MCC:0000358
CLDB; cl3664
CLDB; cl5235
Anatomy/cell type resources BTO; BTO:0003297
Biological sample resources BioSample; SAMN03151747
Chemistry resources ChEMBL-Cells; CHEMBL4295406
ChEMBL-Targets; CHEMBL4296478
PubChem_Cell_line; CVCL_0461
Encyclopedic resources Wikidata; Q54908270
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number26