ID   NCTC 2544
AC   CVCL_0461
SY   NCTC-2544; NCTC2544; NCTC strain 2544
DR   BTO; BTO:0003297
DR   CLO; CLO_0008132
DR   MCCL; MCC:0000358
DR   CLDB; cl3664
DR   CLDB; cl5235
DR   BioSample; SAMN03151747
DR   ChEMBL-Cells; CHEMBL4295406
DR   ChEMBL-Targets; CHEMBL4296478
DR   ICLC; HL97002
DR   PubChem_Cell_line; CVCL_0461
DR   Wikidata; Q54908270
RX   DOI=10.1007/BF02618370;
RX   PubMed=565190;
RX   PubMed=566722;
RX   PubMed=990102;
RX   PubMed=1246601;
RX   PubMed=4864103;
RX   PubMed=5641128;
RX   PubMed=5668122;
RX   PubMed=20143388;
CC   Problematic cell line: Contaminated. Shown to be a HeLa derivative (PubMed=4864103; PubMed=5641128; PubMed=566722; PubMed=1246601; PubMed=20143388). Originally thought to be a normal skin epithelium cell line.
CC   Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00012.
CC   Population: African American.
CC   Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
CC   Discontinued: ATCC; CCL-19; true.
CC   Discontinued: ICLC; HL97002; true.
CC   Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
DI   NCIt; C27677; Human papillomavirus-related cervical adenocarcinoma
OX   NCBI_TaxID=9606; ! Homo sapiens (Human)
HI   CVCL_0030 ! HeLa
SX   Female
AG   30Y6M
CA   Cancer cell line
DT   Created: 04-04-12; Last updated: 02-05-24; Version: 26
RX   DOI=10.1007/BF02618370;
RA   Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.;
RT   "The animal cell culture collection.";
RL   In Vitro 5:1-16(1970).
RX   PubMed=565190; DOI=10.1007/BF00455372;
RA   Neufahrt A., Forster F.J., Gottschalk K., Leonhardi G.;
RT   "Long-term tissue culture of epithelial-like cells from human skin
RT   (NCTC strain 2544). II. Viscosity changes after enzyme treatment.";
RL   Arch. Dermatol. Res. 261:27-31(1978).
RX   PubMed=566722; DOI=10.1007/BF02616110;
RA   Lavappa K.S.;
RT   "Survey of ATCC stocks of human cell lines for HeLa contamination.";
RL   In Vitro 14:469-475(1978).
RX   PubMed=990102; DOI=10.1007/BF00572490;
RA   Neufahrt A., Forster F.J., Heine H., Schaeg G., Leonhardi G.;
RT   "Long-term tissue culture of epithelial-like cells from human skin
RT   (NCTC strain 2544). I. Measurement of viscosity.";
RL   Arch. Dermatol. Res. 256:255-260(1976).
RX   PubMed=1246601; DOI=10.1126/science.1246601;
RA   Nelson-Rees W.A., Flandermeyer R.R.;
RT   "HeLa cultures defined.";
RL   Science 191:96-98(1976).
RX   PubMed=4864103;
RA   Gartler S.M.;
RT   "Genetic markers as tracers in cell culture.";
RL   Natl. Cancer Inst. Monogr. 26:167-195(1967).
RX   PubMed=5641128; DOI=10.1038/217750a0;
RA   Gartler S.M.;
RT   "Apparent Hela cell contamination of human heteroploid cell lines.";
RL   Nature 217:750-751(1968).
RX   PubMed=5668122; DOI=10.3181/00379727-128-33119;
RA   Peterson W.D. Jr., Stulberg C.S., Swanborg N.K., Robinson A.R.;
RT   "Glucose-6-phosphate dehydrogenase isoenzymes in human cell cultures
RT   determined by sucrose-agar gel and cellulose acetate zymograms.";
RL   Proc. Soc. Exp. Biol. Med. 128:772-776(1968).
RX   PubMed=20143388; DOI=10.1002/ijc.25242;
RA   Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A.,
RA   MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R.,
RA   Freshney R.I.;
RT   "Check your cultures! A list of cross-contaminated or misidentified
RT   cell lines.";
RL   Int. J. Cancer 127:1-8(2010).