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Cellosaurus MT58 (CVCL_0442)

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Cell line name MT58
Synonyms MT-58; CHO-MT58; CHO MT58; Mutant 58; Strain 58; CHO strain 58; CHO-58
Accession CVCL_0442
Resource Identification Initiative To cite this cell line use: MT58 (RRID:CVCL_0442)
Comments Characteristics: Displays a thermo-sensitive phenotype. At a permissive temperature (33 Celsius), the cells grow at a rate of about 80% of the parental line while maintaining 80-90% phosphatidylcholine levels. At a non-permissive temperature (40 Celsius), the endogenous Pcyt1a/CCT activity decreases dramatically, blocking membrane synthesis and ultimately leading to apoptosis.
Transformant: ChEBI; CHEBI_23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
Cell type: Epithelial cell of ovary; CL=CL_2000064.
Sequence variations
  • Mutation; UniProtKB; P49584; Pcyt1a; Simple; p.Arg140His (c.419G>A); Zygosity=Unspecified (PubMed=8185307).
Species of origin Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) (NCBI Taxonomy: 10029)
Hierarchy Parent: CVCL_0214 (CHO-K1)
Sex of cell Female
Age at sampling Adult
Category Spontaneously immortalized cell line
Web pages Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/c/cell-lines-detail-189.html
Publications

PubMed=6254065; DOI=10.1073/pnas.77.9.5192; PMCID=PMC350023
Jeffrey David Esko, Christian Rudolf Hubert Raetz;
Autoradiographic detection of animal cell membrane mutants altered in phosphatidylcholine synthesis.
Proc. Natl. Acad. Sci. U.S.A. 77:5192-5196(1980)

PubMed=6265447; DOI=10.1016/S0021-9258(19)68975-1
Jeffrey David Esko, Mary M. Wermuth, Christian Rudolf Hubert Raetz;
Thermolabile CDP-choline synthetase in an animal cell mutant defective in lecithin formation.
J. Biol. Chem. 256:7388-7393(1981)

PubMed=8185307; DOI=10.1006/abbi.1994.1215
Thomas D. Sweitzer, Claudia M. Kent;
Expression of wild-type and mutant rat liver CTP: phosphocholine cytidylyltransferase in a cytidylyltransferase-deficient Chinese hamster ovary cell line.
Arch. Biochem. Biophys. 311:107-116(1994)

PubMed=26083347; DOI=10.1371/journal.pone.0129632; PMCID=PMC4470507
Livia Marton, Gergely N. Nagy, Oliver Ozohanics, Aniko Labas, Balazs Kramos, Julianna Olah, Karoly Vekey, Beata Grolmuszne Vertessy;
Molecular mechanism for the thermo-sensitive phenotype of CHO-MT58 cell line harbouring a mutant CTP:phosphocholine cytidylyltransferase.
PLoS ONE 10:e0129632.1-e0129632.17(2015)

Cross-references
Cell line databases/resources MCCL; MCC:0000338
TOKU-E; 4018
Encyclopedic resources Wikidata; Q54906979
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number17