ID   MT58
AC   CVCL_0442
SY   MT-58; CHO-MT58; CHO MT58; Mutant 58; Strain 58; CHO strain 58; CHO-58
DR   MCCL; MCC:0000338
DR   TOKU-E; 4018
DR   Wikidata; Q54906979
RX   PubMed=6254065;
RX   PubMed=6265447;
RX   PubMed=8185307;
RX   PubMed=26083347;
WW   Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/c/cell-lines-detail-189.html
CC   Characteristics: Displays a thermo-sensitive phenotype. At a permissive temperature (33 Celsius), the cells grow at a rate of about 80% of the parental line while maintaining 80-90% phosphatidylcholine levels. At a non-permissive temperature (40 Celsius), the endogenous Pcyt1a/CCT activity decreases dramatically, blocking membrane synthesis and ultimately leading to apoptosis.
CC   Sequence variation: Mutation; UniProtKB; P49584; Pcyt1a; Simple; p.Arg140His (c.419G>A); Zygosity=Unspecified (PubMed=8185307).
CC   Transformant: ChEBI; CHEBI_23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
CC   Derived from site: In situ; Ovary; UBERON=UBERON_0000992.
CC   Cell type: Epithelial cell of ovary; CL=CL_2000064.
OX   NCBI_TaxID=10029; ! Cricetulus griseus (Chinese hamster)
HI   CVCL_0214 ! CHO-K1
SX   Female
AG   Adult
CA   Spontaneously immortalized cell line
DT   Created: 04-04-12; Last updated: 10-04-25; Version: 17
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RX   PubMed=6265447; DOI=10.1016/S0021-9258(19)68975-1;
RA   Esko, Jeffrey David
RA   Wermuth, Mary M.
RA   Raetz, Christian Rudolf Hubert
RT   "Thermolabile CDP-choline synthetase in an animal cell mutant
RT   defective in lecithin formation.";
RL   J. Biol. Chem. 256:7388-7393(1981).
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RX   PubMed=6254065; DOI=10.1073/pnas.77.9.5192; PMCID=PMC350023;
RA   Esko, Jeffrey David
RA   Raetz, Christian Rudolf Hubert
RT   "Autoradiographic detection of animal cell membrane mutants altered in
RT   phosphatidylcholine synthesis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 77:5192-5196(1980).
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RX   PubMed=8185307; DOI=10.1006/abbi.1994.1215;
RA   Sweitzer, Thomas D.
RA   Kent, Claudia M.
RT   "Expression of wild-type and mutant rat liver CTP: phosphocholine
RT   cytidylyltransferase in a cytidylyltransferase-deficient Chinese
RT   hamster ovary cell line.";
RL   Arch. Biochem. Biophys. 311:107-116(1994).
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RX   PubMed=26083347; DOI=10.1371/journal.pone.0129632; PMCID=PMC4470507;
RA   Marton, Livia
RA   Nagy, Gergely N.
RA   Ozohanics, Oliver
RA   Labas, Aniko
RA   Kramos, Balazs
RA   Olah, Julianna
RA   Vekey, Karoly
RA   Vertessy, Beata Grolmuszne
RT   "Molecular mechanism for the thermo-sensitive phenotype of CHO-MT58
RT   cell line harbouring a mutant CTP:phosphocholine
RT   cytidylyltransferase.";
RL   PLoS ONE 10:e0129632.1-e0129632.17(2015).
//