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Cellosaurus MDAH 2774 (CVCL_0420)

[Text version]
Cell line name MDAH 2774
Synonyms MDAH-2774; MDAH2774; MDA-H2774; 2774; #2774; OV-2774; OV-CA-2774; SK-2774
Accession CVCL_0420
Resource Identification Initiative To cite this cell line use: MDAH 2774 (RRID:CVCL_0420)
Comments Part of: OCCP ovarian cancer cell line panel.
Registration: International Depositary Authority, American Type Culture Collection (ATCC); CRL-10303.
Omics: Deep proteome analysis.
Omics: Transcriptome analysis by microarray.
Derived from sampling site: Ascites.
Sequence variations
  • Mutation; HGNC; 16712; FBXW7; Simple; c.862-1G>A; Zygosity=Heterozygous; Note=Splice acceptor mutation (PubMed=11565033).
  • Mutation; HGNC; 6407; KRAS; Simple; p.Gly12Val (c.35G>T); ClinVar=VCV000012583; Zygosity=Unspecified (PubMed=12068308; PubMed=22705003).
  • Mutation; HGNC; 11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Unspecified (PubMed=8557231; PubMed=25230021).
Disease Ovarian endometrioid adenocarcinoma (NCIt: C7979)
Endometrioid carcinoma of ovary (ORDO: Orphanet_454723)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Sex of cell Female
Age at sampling Age unspecified
Category Cancer cell line
STR profile Source(s): AddexBio; ATCC; PubMed=25230021

CSF1PO10,11 (PubMed=25230021)
10,14 (AddexBio; ATCC)
D5S81810,11,12 (PubMed=25230021)
10,12,13 (AddexBio; ATCC)
D7S8208,9.3,10.1,11 (AddexBio; ATCC)
10,11,12 (PubMed=25230021)
D13S3178,9 (PubMed=25230021)
8,9,12 (AddexBio; ATCC)
D16S53910,11,12 (PubMed=25230021)
10,11,12,14 (AddexBio; ATCC)
Penta D13,14
Penta E13,14
TPOX8 (PubMed=25230021)
8,11 (AddexBio; ATCC)
vWA14,16,19,21 (AddexBio; ATCC)
15,19 (PubMed=25230021)

Run an STR similarity search on this cell line

PubMed=719612; DOI=10.1002/1097-0142(197811)42:5<2352::AID-CNCR2820420536>3.0.CO;2-#
Freedman R.S., Pihl E., Kusyk C., Gallager H.S., Rutledge F.N.
Characterization of an ovarian carcinoma cell line.
Cancer 42:2352-2359(1978)

PubMed=6582512; DOI=10.1073/pnas.81.2.568
Mattes M.J., Cordon-Cardo C., Lewis J.L. Jr., Old L.J., Lloyd K.O.
Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.
Proc. Natl. Acad. Sci. U.S.A. 81:568-572(1984)

PubMed=8557231; DOI=10.1006/gyno.1996.0014
Skilling J.S., Squatrito R.C., Connor J.P., Niemann T., Buller R.E.
p53 gene mutation analysis and antisense-mediated growth inhibition of human ovarian carcinoma cell lines.
Gynecol. Oncol. 60:72-80(1996)

PubMed=9698466; DOI=10.1006/gyno.1998.5039
Maxwell G.L., Risinger J.I., Tong B., Shaw H., Barrett J.C., Berchuck A., Futreal P.A.
Mutation of the PTEN tumor suppressor gene is not a feature of ovarian cancers.
Gynecol. Oncol. 70:13-16(1998)

PubMed=11314036; DOI=10.1038/sj.onc.1204211
Forgacs E., Wren J.D., Kamibayashi C., Kondo M., Xu X.L., Markowitz S.D., Tomlinson G.E., Muller C.Y., Gazdar A.F., Garner H.R., Minna J.D.
Searching for microsatellite mutations in coding regions in lung, breast, ovarian and colorectal cancers.
Oncogene 20:1005-1009(2001)

PubMed=11565033; DOI=10.1038/35095068
Moberg K.H., Bell D.W., Wahrer D.C.R., Haber D.A., Hariharan I.K.
Archipelago regulates cyclin E levels in Drosophila and is mutated in human cancer cell lines.
Nature 413:311-316(2001)

PubMed=12068308; DOI=10.1038/nature00766
Davies H., Bignell G.R., Cox C., Stephens P.J., Edkins S., Clegg S., Teague J.W., Woffendin H., Garnett M.J., Bottomley W., Davis N., Dicks E., Ewing R., Floyd Y., Gray K., Hall S., Hawes R., Hughes J., Kosmidou V., Menzies A., Mould C., Parker A., Stevens C., Watt S., Hooper S., Wilson R., Jayatilake H., Gusterson B.A., Cooper C.S., Shipley J.M., Hargrave D., Pritchard-Jones K., Maitland N.J., Chenevix-Trench G., Riggins G.J., Bigner D.D., Palmieri G., Cossu A., Flanagan A.M., Nicholson A., Ho J.W.C., Leung S.Y., Yuen S.T., Weber B.L., Seigler H.F., Darrow T.L., Paterson H.F., Marais R., Marshall C.J., Wooster R., Stratton M.R., Futreal P.A.
Mutations of the BRAF gene in human cancer.
Nature 417:949-954(2002)

PubMed=15153938; DOI=10.1038/sj.cgt.7700727
Quist S.R., Wang-Gohrke S., Kohler T., Kreienberg R., Runnebaum I.B.
Cooperative effect of adenoviral p53 gene therapy and standard chemotherapy in ovarian cancer cells independent of the endogenous p53 status.
Cancer Gene Ther. 11:547-554(2004)

PubMed=19288585; DOI=10.1002/elps.200800505
Dai L., Li C., Shedden K.A., Misek D.E., Lubman D.M.
Comparative proteomic study of two closely related ovarian endometrioid adenocarcinoma cell lines using cIEF fractionation and pathway analysis.
Electrophoresis 30:1119-1131(2009)

PubMed=22328975; DOI=10.1158/2159-8290.CD-11-0170
Hanrahan A.J., Schultz N., Westfal M.L., Sakr R.A., Giri D.D., Scarperi S., Janakiraman M., Olvera N., Stevens E.V., She Q.-B., Aghajanian C., King T.A., de Stanchina E., Spriggs D.R., Heguy A., Taylor B.S., Sander C., Rosen N., Levine D.A., Solit D.B.
Genomic complexity and AKT dependence in serous ovarian cancer.
Cancer Discov. 2:56-67(2012)

PubMed=22705003; DOI=10.1016/j.humpath.2012.03.011
Rahman M., Nakayama K., Rahman M.T., Nakayama N., Ishikawa M., Katagiri A., Iida K., Nakayama S., Otsuki Y., Shih I.-M., Miyazaki K.
Clinicopathologic and biological analysis of PIK3CA mutation in ovarian clear cell carcinoma.
Hum. Pathol. 43:2197-2206(2012)

PubMed=22710073; DOI=10.1016/j.ygyno.2012.06.017
Korch C.T., Spillman M.A., Jackson T.A., Jacobsen B.M., Murphy S.K., Lessey B.A., Jordan V.C., Bradford A.P.
DNA profiling analysis of endometrial and ovarian cell lines reveals misidentification, redundancy and contamination.
Gynecol. Oncol. 127:241-248(2012)

PubMed=25230021; DOI=10.1371/journal.pone.0103988
Beaufort C.M., Helmijr J.C.A., Piskorz A.M., Hoogstraat M., Ruigrok-Ritstier K., Besselink N., Murtaza M., van IJcken W.F.J., Heine A.A.J., Smid M., Koudijs M.J., Brenton J.D., Berns E.M.J.J., Helleman J.
Ovarian cancer cell line panel (OCCP): clinical importance of in vitro morphological subtypes.
PLoS ONE 9:E103988-E103988(2014)

PubMed=27235858; DOI=10.1016/j.ygyno.2016.05.028
Hernandez L., Kim M.K., Lyle L.T., Bunch K.P., House C.D., Ning F., Noonan A.M., Annunziata C.M.
Characterization of ovarian cancer cell lines as in vivo models for preclinical studies.
Gynecol. Oncol. 142:332-340(2016)

Cell line collections AddexBio; C0017001/57
BCRJ; 0163 - Discontinued
Cell line databases/resources MCCL; MCC:0000317
Lonza; 1433
Anatomy/cell type resources BTO; BTO:0003942
Chemistry resources ChEMBL-Cells; CHEMBL4483147
ChEMBL-Targets; CHEMBL4483256
PubChem_Cell_line; CVCL_0420
Encyclopedic resources Wikidata; Q54904669
Gene expression databases GEO; GSE27654
GEO; GSM1291124
Polymorphism and mutation databases Cosmic; 724881
Cosmic; 844345
Cosmic; 875291
Cosmic; 897431
Cosmic; 923109
Cosmic; 1044237
Cosmic; 1113282
Cosmic; 1524360
Cosmic; 1708386
Cosmic; 1709262
Cosmic; 2186588
IARC_TP53; 5550
IARC_TP53; 21489
Entry history
Entry creation04-Apr-2012
Last entry update21-Mar-2023
Version number31