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Cellosaurus M059J (CVCL_0400)

[Text version]
Cell line name M059J
Synonyms Mo 59J; MO59J
Accession CVCL_0400
Resource Identification Initiative To cite this cell line use: M059J (RRID:CVCL_0400)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: ENCODE project common cell types; tier 3.
Population: Caucasian.
Characteristics: Radiosensitive (PubMed=7494872).
Karyotypic information: Has a near pentaploid phenotype.
Microsatellite instability: Stable (MSS) (Sanger).
Omics: CRISPR phenotypic screen.
Omics: Deep exome analysis.
Omics: Deep quantitative proteome analysis.
Omics: DNA methylation analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Caution: Was incorrectly stated to have deleterious mutations in ATM in PubMed=12105990.
Derived from site: In situ; Brain; UBERON=UBERON_0000955.
Sequence variations
  • Mutation; HGNC; 9413; PRKDC; Simple; p.Thr1351Profs*8 (c.4051delA); Zygosity=Heterozygous (PubMed=11418067; DepMap=ACH-001118).
  • Mutation; HGNC; 9588; PTEN; Simple; p.Pro204fs*17 (c.610delC); Zygosity=Homozygous (ATCC=CRL-2366; Cosmic-CLP=949094; DepMap=ACH-001118).
  • Mutation; HGNC; 11998; TP53; Simple; p.Glu286lys (c.856G>A); Zygosity=Homozygous (PubMed=11023613; Cosmic-CLP=949094; DepMap=ACH-001118).
HLA typing Source: PubMed=26589293
Class I
Class II
Genome ancestry Source: PubMed=30894373

Origin% genome
Native American0
East Asian, North2.75
East Asian, South0
South Asian0
European, North67.68
European, South28.55
Disease Glioblastoma (NCIt: C3058)
Glioblastoma (ORDO: Orphanet_360)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Originate from same individual CVCL_0401 ! M059K
Sex of cell Male
Age at sampling 33Y
Category Cancer cell line
STR profile Source(s): ATCC=CRL-2366; Cosmic-CLP=949094; PubMed=25877200

AmelogeninX (PubMed=25877200)
X,Y (ATCC=CRL-2366; Cosmic-CLP=949094)
CSF1PO10 (PubMed=25877200)
10,12 (ATCC=CRL-2366; Cosmic-CLP=949094)
D3S135814 (PubMed=25877200)
14,15 (ATCC=CRL-2366)
D5S81811,12 (ATCC=CRL-2366; Cosmic-CLP=949094)
12 (PubMed=25877200)
D7S82010 (PubMed=25877200)
10,12 (ATCC=CRL-2366; Cosmic-CLP=949094)
Penta D12,13
Penta E7

Run an STR similarity search on this cell line
Web pages http://genome.ucsc.edu/ENCODE/protocols/cell/human/M059J_Stam_protocol.pdf

PubMed=8316628; DOI=10.2307/3578196
Allalunis-Turner M.J., Barron G.M., Day R.S. III, Dobler K.D., Mirzayans R.
Isolation of two cell lines from a human malignant glioma specimen differing in sensitivity to radiation and chemotherapeutic drugs.
Radiat. Res. 134:349-354(1993)

PubMed=7494872; DOI=10.2307/3578948
Allalunis-Turner M.J., Zia P.K.Y., Barron G.M., Mirzayans R., Day R.S. III
Radiation-induced DNA damage and repair in cells of a radiosensitive human malignant glioma cell line.
Radiat. Res. 144:288-293(1995)

PubMed=10629611; DOI=10.1667/0033-7587(2000)153[0125:COTRMC]2.0.CO;2
Hoppe B.S., Jensen R.B., Kirchgessner C.U.
Complementation of the radiosensitive M059J cell line.
Radiat. Res. 153:125-130(2000)

PubMed=11023613; DOI=10.1667/0033-7587(2000)154[0473:HTFTMG]2.0.CO;2
Anderson C.W., Allalunis-Turner M.J.
Human TP53 from the malignant glioma-derived cell lines M059J and M059K has a cancer-associated mutation in exon 8.
Radiat. Res. 154:473-476(2000)

PubMed=11418067; DOI=10.1667/0033-7587(2001)156[0002:FMIPTG]2.0.CO;2
Anderson C.W., Dunn J.J., Freimuth P.I., Galloway A.M., Allalunis-Turner M.J.
Frameshift mutation in PRKDC, the gene for DNA-PKcs, in the DNA repair-defective, human, glioma-derived cell line M059J.
Radiat. Res. 156:2-9(2001)

PubMed=12105990; DOI=10.1667/0033-7587(2002)158[0195:DOAGMI]2.0.CO;2
Tsuchida R., Yamada T., Takagi M., Shimada A., Ishioka C., Katsuki Y., Igarashi T., Chessa L., Delia D., Teraoka H., Mizutani S.
Detection of ATM gene mutation in human glioma cell line M059J by a rapid frameshift/stop codon assay in yeast.
Radiat. Res. 158:195-201(2002)

PubMed=18757420; DOI=10.1158/0008-5472.CAN-08-0545
Burdak-Rothkamm S., Rothkamm K., Prise K.M.
ATM acts downstream of ATR in the DNA damage response signaling of bystander cells.
Cancer Res. 68:7059-7065(2008)

PubMed=20164919; DOI=10.1038/nature08768
Bignell G.R., Greenman C.D., Davies H., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Behan F.M., Iorio F., Picco G., Goncalves E., Beaver C.M., Migliardi G., Santos R., Rao Y., Sassi F., Pinnelli M., Ansari R., Harper S., Jackson D.A., McRae R., Pooley R., Wilkinson P., van der Meer D.J., Dow D., Buser-Doepner C.A., Bertotti A., Trusolino L., Stronach E.A., Saez-Rodriguez J., Yusa K., Garnett M.J.
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

Cell line collections (Providers) ATCC; CRL-2366
BCRC; 60382
BCRJ; 0151
Cell line databases/resources CLO; CLO_0007445
MCCL; MCC:0000294
cancercelllines; CVCL_0400
Cell_Model_Passport; SIDM00659
Cosmic-CLP; 949094
DepMap; ACH-001118
Biological sample resources BioSample; SAMN03471832
BioSample; SAMN03473044
Chemistry resources ChEMBL-Cells; CHEMBL3308113
ChEMBL-Targets; CHEMBL1075494
GDSC; 949094
PharmacoDB; M059J_877_2019
PubChem_Cell_line; CVCL_0400
Encyclopedic resources Wikidata; Q54903415
Experimental variables resources EFO; EFO_0005697
Gene expression databases ArrayExpress; E-MTAB-783
ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-3610
GEO; GSM326251
GEO; GSM1374640
GEO; GSM1670070
Polymorphism and mutation databases Cosmic; 949094
Cosmic; 1746953
Cosmic; 2367537
IARC_TP53; 13244
Progenetix; CVCL_0400
Proteomic databases PRIDE; PXD030304
Sequence databases EGA; EGAS00001000610
EGA; EGAS00001000978
Entry history
Entry creation04-Apr-2012
Last entry update02-May-2024
Version number39