Cellosaurus logo
expasy logo

Cellosaurus KB (CVCL_0372)

[Text version]
Cell line name KB
Synonyms KB (HeLa derivative); HeLa-KB; Strain KB
Accession CVCL_0372
Resource Identification Initiative To cite this cell line use: KB (RRID:CVCL_0372)
Comments Problematic cell line: Contaminated. Shown to be a HeLa derivative (PubMed=4864103; PubMed=5641128; PubMed=1246601; PubMed=20143388). Originally thought to originate from an epidermal carcinoma of the mouth.
Part of: KuDOS 95 cell line panel.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00010.
Population: African American.
Doubling time: ~30 hours (DSMZ=ACC-136).
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Omics: Genomics; Whole genome sequencing; Low read coverage.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related endocervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0030 (HeLa)
Children:
CVCL_D680 (KB 16)CVCL_D681 (KB 18)CVCL_C4YP (KB N10)
CVCL_2088 (KB-3-1)CVCL_F0BE (KB-BLMr)CVCL_M096 (KB-R)
CVCL_HA90 (KB/BF-1)CVCL_HA91 (KB/BF-2)CVCL_AE00 (KB/STP-1)
CVCL_AE01 (KB/STP-2)CVCL_D599 (KBv200)CVCL_U657 (KE2a)
CVCL_6B18 (Kst-6)CVCL_X217 (VJ-300)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
STR profile Source(s): ATCC=CRL-3596; CCRID=1101HUM-PUMC000163; CLS=300446; DSMZ=ACC-136; JCRB=JCRB9027; KCLB=10017; PubMed=11416159; PubMed=17254797; TKG=TKG 0401

Markers:
AmelogeninX
CSF1PO9,10
D2S133817
D3S135815,18
D5S81811,12
D7S8208,12
D8S117912,13
D13S31712,13.3 (ATCC=CRL-3596; CLS=300446; DSMZ=ACC-136; JCRB=JCRB9027; KCLB=10017; TKG=TKG 0401)
12,14 (CCRID=1101HUM-PUMC000163; PubMed=17254797)
D16S5399,10
D18S5116
D19S43313,14
D21S1127,28
FGA21
Penta D8,15
Penta E7,17
TH017
TPOX8,12
vWA16,18

Run an STR similarity search on this cell line
Web pages Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/k/cell-lines-detail-494.html
Publications

PubMed=13254761; DOI=10.3181/00379727-89-21811
Harry Eagle;
Propagation in a fluid medium of a human epidermoid carcinoma, strain KB.
Proc. Soc. Exp. Biol. Med. 89:362-364(1955)

PubMed=13322936; DOI=10.3181/00379727-91-22263
Harry Eagle, Karl Habel, Wallace Prescott Rowe, Robert Joseph Huebner;
Viral susceptibility of a human carcinoma cell (strain KB).
Proc. Soc. Exp. Biol. Med. 91:361-364(1956)

PubMed=13431913; DOI=10.3181/00379727-94-23044
George E. Foley, Alfred H. Handler;
Differentiation of normal and neoplastic cells maintained in tissue culture by implantation into normal hamsters.
Proc. Soc. Exp. Biol. Med. 94:661-664(1957)

PubMed=14185313; DOI=10.1126/science.146.3641.241
Cell Culture Collection Committee
Animal cell strains. The Cell Culture Collection Committee has assembled and certified 23 strains of animal cells.
Science 146:241-243(1964)

PubMed=4864103
Stanley Michael Gartler;
Genetic markers as tracers in cell culture.
Natl. Cancer Inst. Monogr. 26:167-195(1967)

PubMed=5641128; DOI=10.1038/217750a0
Stanley Michael Gartler;
Apparent Hela cell contamination of human heteroploid cell lines.
Nature 217:750-751(1968)

PubMed=5668122; DOI=10.3181/00379727-128-33119
Ward Davis Peterson Jr., Cyril S. Stulberg, N.K. Swanborg, Andria Rasile Robinson;
Glucose-6-phosphate dehydrogenase isoenzymes in human cell cultures determined by sucrose-agar gel and cellulose acetate zymograms.
Proc. Soc. Exp. Biol. Med. 128:772-776(1968)

DOI=10.1007/BF02618370
Cyril S. Stulberg, Lewis Lemon Coriell, Alexis John Kniazeff, John E. Shannon;
The animal cell culture collection.
In Vitro 5:1-16(1970)

PubMed=4203458
John C. Petricciani, Roslyn Elizabeth Wallace, Donald W. McCoy;
A comparison of three in vivo assays for cell tumorigenicity.
Cancer Res. 34:105-108(1974)

DOI=10.1007/978-1-4757-1647-4_13
June Lee Biedler;
Chromosome abnormalities in human tumor cells in culture.
(In book chapter) Human tumor cells in vitro; Fogh, Jorgen (eds.); pp.359-394; Springer; New York; USA (1975)

PubMed=1246601; DOI=10.1126/science.1246601
Walter Anthony Nelson-Rees, Robert R. Flandermeyer;
HeLa cultures defined.
Science 191:96-98(1976)

PubMed=656543; DOI=10.1016/S0006-3495(78)85487-3; PMCID=PMC1473442
Ursula Kristin Ehmann, Kem H. Cook, Errol Clive Friedberg;
The kinetics of thymine dimer excision in ultraviolet-irradiated human cells.
Biophys. J. 22:249-264(1978)

PubMed=6988327; DOI=10.1007/BF02831503
Stephen James O'Brien, John E. Shannon, Mitchell H. Gail;
A molecular approach to the identification and individualization of human and animal cells in culture: isozyme and allozyme genetic signatures.
In Vitro 16:119-135(1980)

PubMed=8397027
Hajime Ogura, Mitsuo Yoshinouchi, Takafumi Kudo, Makoto Imura, Tazuko Fujiwara, Yoshiro Yabe;
Human papillomavirus type 18 DNA in so-called HEP-2, KB and FL cells -- further evidence that these cells are HeLa cell derivatives.
Cell. Mol. Biol. 39:463-467(1993)

PubMed=11416159; DOI=10.1073/pnas.121616198; PMCID=PMC35459
John R.W. Masters, Jim A. Thomson, Bernadette Daly-Burns, Yvonne A. Reid, Wilhelm Gerhard Dirks, Phil Packer, Lorraine H. Toji, Tadao Ohno, Hideyuki Tanabe, Colin Francis Arlett, Lloyd R. Kelland ...Show all 16 authors... , Maureen Harrison, Arvind K. Virmani, Timothy H. Ward, Karen L. Ayres, Paul G. Debenham; Show fewer authors
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

PubMed=11668190; DOI=10.1177/002215540104901105
Hilmar Quentmeier, Mary Osborn, Julia Reinhardt, Margarete Zaborski, Hans Gunther Drexler;
Immunocytochemical analysis of cell lines derived from solid tumors.
J. Histochem. Cytochem. 49:1369-1378(2001)

PubMed=17254797; DOI=10.1016/j.biologicals.2006.10.001
Shahram Azari, Nahid Ahmadi, Mahmood Jeddi-Tehrani, Fazel Shokri;
Profiling and authentication of human cell lines using short tandem repeat (STR) loci: report from the National Cell Bank of Iran.
Biologicals 35:195-202(2007)

PubMed=17325662; DOI=10.1038/sj.onc.1210330
K. Nakaya, Hidehisa D. Yamagata, Norimasa Arita, Koh-ichi Nakashiro, Masato Nose, Tetsuro Miki, Hiroyuki Hamakawa;
Identification of homozygous deletions of tumor suppressor gene FAT in oral cancer using CGH-array.
Oncogene 26:5300-5308(2007)

PubMed=20143388; DOI=10.1002/ijc.25242
Amanda Capes-Davis, George Theodosopoulos, Isobel Atkin, Hans Gunther Drexler, Arihiro Kohara, Roderick A.F. MacLeod, John R.W. Masters, Yukio Nakamura, Yvonne A. Reid, Roger Robert Reddel, Robert Ian Freshney;
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=23925245; DOI=10.1038/nature12064; PMCID=PMC3740412
Andrew C. Adey, Joshua N. Burton, Jacob O. Kitzman, Joseph B. Hiatt, Alexandra P. Lewis, Beth K. Martin, Ruo-Lan Qiu, Choli Lee, Jay Shendure;
The haplotype-resolved genome and epigenome of the aneuploid HeLa cancer cell line.
Nature 500:207-211(2013)

CLPUB00484
Zhi-Hong Xu, Wen-Wen Sun, Jun-Biao Hang, Bei-Li Gao, Jia-An Hu;
RNA-seq identified a novel functional MET fusion in head and neck cancer 5-8F cell line.
Int. J. Clin. Exp. Pathol. 9:8308-8317(2016)

PubMed=28455420; DOI=10.1158/0008-5472.CAN-16-2258
Liwen Vaughan, Wolfgang Glanzel, Christopher T. Korch, Amanda Capes-Davis;
Widespread use of misidentified cell line KB (HeLa): incorrect attribution and its impact revealed through mining the scientific literature.
Cancer Res. 77:2784-2788(2017)

Cross-references
Cell line collections (Providers) ATCC; CCL-17 - Discontinued
ATCC; CRL-3596
BCRC; 60017 - Discontinued
BCRJ; 0128
CCLV; CCLV-RIE 0016
CLS; 300446
DSMZ; ACC-136 - Discontinued
ECACC; 86103004 - Discontinued
ECACC; 94050408
ICLC; HTL96014 - Discontinued
IZSLER; BS TCL 34
JCRB; JCRB9027
KCB; KCB 94020YJ
KCLB; 10017
NCBI_Iran; C152
RCB; RCB1888 - Discontinued
TKG; TKG 0401
Cell line databases/resources CLO; CLO_0007074
MCCL; MCC:0000267
CLDB; cl3001
CLDB; cl3002
CLDB; cl3003
CLDB; cl3004
CLDB; cl3005
CLDB; cl3006
CCRID; 1101HUM-PUMC000163
DSMZCellDive; ACC-136
TOKU-E; 2094
Anatomy/cell type resources BTO; BTO_0000665
Biological sample resources BioSample; SAMN03151730
Chemistry resources ChEMBL-Cells; CHEMBL3307959
ChEMBL-Targets; CHEMBL398
PubChem_Cell_line; CVCL_0372
Encyclopedic resources Wikidata; Q28334825
Gene expression databases GEO; GSM1374595
GEO; GSM1374596
Medical resources MeSH; D007624
Polymorphism and mutation databases Cosmic; 753567
Cosmic; 1042301
Cosmic; 1339927
Cosmic; 1969496
Cosmic; 2301581
Cosmic; 2546843
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number42