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Cellosaurus KB (CVCL_0372)

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Cell line name KB
Synonyms KB (HeLa derivative); HeLa-KB; Strain KB
Accession CVCL_0372
Resource Identification Initiative To cite this cell line use: KB (RRID:CVCL_0372)
Comments Problematic cell line: Contaminated. Shown to be a HeLa derivative (PubMed=4864103; PubMed=5641128; PubMed=1246601; PubMed=20143388). Originally thought to originate from an epidermal carcinoma of the mouth.
Part of: KuDOS 95 cell line panel.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00010.
Population: African American.
Doubling time: ~30 hours (DSMZ=ACC-136).
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Omics: Genome sequenced (low read coverage).
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related cervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0030 (HeLa)
CVCL_D680 (KB 16)CVCL_D681 (KB 18)CVCL_C4YP (KB N10)
CVCL_2088 (KB-3-1)CVCL_M096 (KB-R)CVCL_HA90 (KB/BF-1)
CVCL_D599 (KBv200)CVCL_U657 (KE2a)CVCL_6B18 (Kst-6)
CVCL_X217 (VJ-300)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
STR profile Source(s): ATCC; CCRID; CLS; DSMZ; JCRB; KCLB; PubMed=11416159; PubMed=17254797; TKG

D13S31712,13.3 (ATCC; CLS; DSMZ; JCRB; KCLB; TKG)
12,14 (CCRID; PubMed=17254797)
Penta D8,15
Penta E7,17

Run an STR similarity search on this cell line
Web pages https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/k/cell-lines-detail-494.html

PubMed=13254761; DOI=10.3181/00379727-89-21811
Eagle H.
Propagation in a fluid medium of a human epidermoid carcinoma, strain KB.
Proc. Soc. Exp. Biol. Med. 89:362-364(1955)

PubMed=13322936; DOI=10.3181/00379727-91-22263
Eagle H., Habel K., Rowe W.P., Huebner R.J.
Viral susceptibility of a human carcinoma cell (strain KB).
Proc. Soc. Exp. Biol. Med. 91:361-364(1956)

PubMed=13431913; DOI=10.3181/00379727-94-23044
Foley G.E., Handler A.H.
Differentiation of normal and neoplastic cells maintained in tissue culture by implantation into normal hamsters.
Proc. Soc. Exp. Biol. Med. 94:661-664(1957)

PubMed=14185313; DOI=10.1126/science.146.3641.241
Cell Culture Collection Committee
Animal cell strains. The Cell Culture Collection Committee has assembled and certified 23 strains of animal cells.
Science 146:241-243(1964)

Gartler S.M.
Genetic markers as tracers in cell culture.
Natl. Cancer Inst. Monogr. 26:167-195(1967)

PubMed=5641128; DOI=10.1038/217750a0
Gartler S.M.
Apparent Hela cell contamination of human heteroploid cell lines.
Nature 217:750-751(1968)

PubMed=5668122; DOI=10.3181/00379727-128-33119
Peterson W.D. Jr., Stulberg C.S., Swanborg N.K., Robinson A.R.
Glucose-6-phosphate dehydrogenase isoenzymes in human cell cultures determined by sucrose-agar gel and cellulose acetate zymograms.
Proc. Soc. Exp. Biol. Med. 128:772-776(1968)

Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.
The animal cell culture collection.
In Vitro 5:1-16(1970)

Petricciani J.C., Wallace R.E., McCoy D.W.
A comparison of three in vivo assays for cell tumorigenicity.
Cancer Res. 34:105-108(1974)

Biedler J.L.
Chromosome abnormalities in human tumor cells in culture.
(In) Human tumor cells in vitro; Fogh J. (eds.); pp.359-394; Springer; New York (1975)

PubMed=1246601; DOI=10.1126/science.1246601
Nelson-Rees W.A., Flandermeyer R.R.
HeLa cultures defined.
Science 191:96-98(1976)

PubMed=656543; DOI=10.1016/S0006-3495(78)85487-3
Ehmann U.K., Cook K.H., Friedberg E.C.
The kinetics of thymine dimer excision in ultraviolet-irradiated human cells.
Biophys. J. 22:249-264(1978)

PubMed=6988327; DOI=10.1007/BF02831503
O'Brien S.J., Shannon J.E., Gail M.H.
A molecular approach to the identification and individualization of human and animal cells in culture: isozyme and allozyme genetic signatures.
In Vitro 16:119-135(1980)

Ogura H., Yoshinouchi M., Kudo T., Imura M., Fujiwara T., Yabe Y.
Human papillomavirus type 18 DNA in so-called HEP-2, KB and FL cells -- further evidence that these cells are HeLa cell derivatives.
Cell. Mol. Biol. 39:463-467(1993)

PubMed=11416159; DOI=10.1073/pnas.121616198
Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G., Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R., Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

PubMed=11668190; DOI=10.1177/002215540104901105
Quentmeier H., Osborn M., Reinhardt J., Zaborski M., Drexler H.G.
Immunocytochemical analysis of cell lines derived from solid tumors.
J. Histochem. Cytochem. 49:1369-1378(2001)

PubMed=17254797; DOI=10.1016/j.biologicals.2006.10.001
Azari S., Ahmadi N., Tehrani M.J., Shokri F.
Profiling and authentication of human cell lines using short tandem repeat (STR) loci: report from the National Cell Bank of Iran.
Biologicals 35:195-202(2007)

PubMed=17325662; DOI=10.1038/sj.onc.1210330
Nakaya K., Yamagata H.D., Arita N., Nakashiro K.-i., Nose M., Miki T., Hamakawa H.
Identification of homozygous deletions of tumor suppressor gene FAT in oral cancer using CGH-array.
Oncogene 26:5300-5308(2007)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=23925245; DOI=10.1038/nature12064
Adey A., Burton J.N., Kitzman J.O., Hiatt J.B., Lewis A.P., Martin B.K., Qiu R.-L., Lee C., Shendure J.
The haplotype-resolved genome and epigenome of the aneuploid HeLa cancer cell line.
Nature 500:207-211(2013)

Xu Z.-H., Sun W.-W., Hang J.-B., Gao B.-L., Hu J.-A.
RNA-seq identified a novel functional MET fusion in head and neck cancer 5-8F cell line.
Int. J. Clin. Exp. Pathol. 9:8308-8317(2016)

PubMed=28455420; DOI=10.1158/0008-5472.CAN-16-2258
Vaughan L., Glanzel W., Korch C.T., Capes-Davis A.
Widespread use of misidentified cell line KB (HeLa): incorrect attribution and its impact revealed through mining the scientific literature.
Cancer Res. 77:2784-2788(2017)

Cell line collections (Providers) ATCC; CCL-17
BCRC; 60017 - Discontinued
BCRJ; 0128
CLS; 300446
DSMZ; ACC-136 - Discontinued
ECACC; 86103004 - Discontinued
ECACC; 94050408
ICLC; HTL96014 - Discontinued
KCB; KCB 94020YJ
KCLB; 10017
NCBI_Iran; C152
RCB; RCB1888 - Discontinued
TKG; TKG 0401
Cell line databases/resources CLO; CLO_0007074
MCCL; MCC:0000267
CLDB; cl3001
CLDB; cl3002
CLDB; cl3003
CLDB; cl3004
CLDB; cl3005
CLDB; cl3006
CCRID; 1101HUM-PUMC000163
DSMZCellDive; ACC-136
TOKU-E; 2094
Anatomy/cell type resources BTO; BTO:0000665
Biological sample resources BioSample; SAMN03151730
Chemistry resources ChEMBL-Cells; CHEMBL3307959
ChEMBL-Targets; CHEMBL398
PubChem_Cell_line; CVCL_0372
Encyclopedic resources Wikidata; Q28334825
Gene expression databases GEO; GSM1374595
GEO; GSM1374596
Medical resources MeSH; D007624
Polymorphism and mutation databases Cosmic; 753567
Cosmic; 1042301
Cosmic; 1339927
Cosmic; 1969496
Cosmic; 2301581
Cosmic; 2546843
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number38