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Cellosaurus KAT-5 (CVCL_0370)

[Text version]
Cell line name KAT-5
Synonyms KAT5
Accession CVCL_0370
Resource Identification Initiative To cite this cell line use: KAT-5 (RRID:CVCL_0370)
Comments Problematic cell line: Contaminated. Shown to be a HT-29 derivative (PubMed=18713817; PubMed=20143388). Originally thought to originate from a thyroid gland papillary carcinoma.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00223.
Population: Caucasian.
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Mutation; HGNC; HGNC:583; APC; Simple; p.Glu853Ter (c.2557G>T); ClinVar=VCV001071632; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:583; APC; Simple; p.Thr1556Asnfs*3 (c.4666dupA) (c.4666_4667insA); ClinVar=VCV000428112; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:1097; BRAF; Simple; p.Val600Glu (c.1799T>A); ClinVar=VCV000013961; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:8975; PIK3CA; Simple; p.Pro449Thr (c.1345C>A); ClinVar=VCV001333250; Zygosity=Heterozygous (from parent cell line).
  • Mutation; HGNC; HGNC:6770; SMAD4; Simple; p.Gln311Ter (c.931C>T); ClinVar=VCV000230663; Zygosity=Homozygous (from parent cell line).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Arg273His (c.818G>A); ClinVar=VCV000012366; Zygosity=Homozygous (from parent cell line).
Disease Colon adenocarcinoma (NCIt: C4349)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0320 (HT-29)
Sex of cell Female
Age at sampling 44Y
Category Cancer cell line
STR profile Source(s): PubMed=18713817

Markers:
AmelogeninX
D3S135815,17
D5S81811,12
D7S82010
D8S117910,16
D13S31711
D18S5113
D21S1130
FGA20,22
vWA17,19

Run an STR similarity search on this cell line
Web pages Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Publications

PubMed=18713817; DOI=10.1210/jc.2008-1102; PMCID=PMC2582569
Rebecca E. Schweppe, Joshua P. Klopper, Christopher T. Korch, Umarani Pugazhenthi, Miriam Benezra, Jeffrey A. Knauf, James Alexander Fagin, Laura A. Marlow, John Alton Copland 3rd, Robert Christian Smallridge, Bryan R. Haugen;
Deoxyribonucleic acid profiling analysis of 40 human thyroid cancer cell lines reveals cross-contamination resulting in cell line redundancy and misidentification.
J. Clin. Endocrinol. Metab. 93:4331-4341(2008)

PubMed=20143388; DOI=10.1002/ijc.25242
Amanda Capes-Davis, George Theodosopoulos, Isobel Atkin, Hans Gunther Drexler, Arihiro Kohara, Roderick A.F. MacLeod, John R.W. Masters, Yukio Nakamura, Yvonne A. Reid, Roger Robert Reddel, Robert Ian Freshney;
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

Cross-references
Cell line databases/resources MCCL; MCC:0000265
cancercelllines; CVCL_0370
Biological sample resources BioSample; SAMN03151791
Encyclopedic resources Wikidata; Q54899533
Polymorphism and mutation databases Cosmic; 888091
Cosmic; 913246
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number29