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Cellosaurus HT-2 clone A5E (CVCL_0319)

[Text version]
Cell line name HT-2 clone A5E
Synonyms HT2-clone-A5E; HT2 Clone A5E
Accession CVCL_0319
Resource Identification Initiative To cite this cell line use: HT-2 clone A5E (RRID:CVCL_0319)
Comments Problematic cell line: Probably misidentified/contaminated. While said to originate from a BALB/c mouse it exhibits a greater similarity with a C57BL strain. Its STR profile has three alleles or more at seven loci, high peak height imbalance and is discordant at eight STR markers. It therefore needs more characterization to determine if it is contaminated or misidentified due to the inconsistencies with the strain of origin and the increase of alleles per marker (PubMed=31220119).
Characteristics: IL2 and IL4 dependent.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: BALB/c.
Hierarchy Parent: CVCL_5T92 (HT-2)
Category Factor-dependent cell line

PubMed=31220119; DOI=10.1371/journal.pone.0218412
Almeida J.L., Dakic A., Kindig K., Kone M., Letham D.L.D., Langdon S., Peat R., Holding-Pillai J., Hall E.M., Ladd M., Shaffer M.D., Berg H., Li J.-L., Wigger G., Lund S., Steffen C.R., Fransway B.B., Geraghty B., Natoli M., Bauer B.A., Gollin S.M., Lewis D.W., Reid Y.A.
Interlaboratory study to validate a STR profiling method for intraspecies identification of mouse cell lines.
PLoS ONE 14:E0218412-E0218412(2019)

Cell line collections (Providers) ATCC; CRL-1841
ECACC; 92021401
Cell line databases/resources CLO; CLO_0004281
CLO; CLO_0004282
MCCL; MCC:0000212
CLDB; cl1745
Encyclopedic resources Wikidata; Q54896508
Entry history
Entry creation04-Apr-2012
Last entry update21-Mar-2023
Version number9