ID   HT-2 clone A5E
AC   CVCL_0319
SY   HT2-clone-A5E; HT2 Clone A5E
DR   CLO; CLO_0004281
DR   CLO; CLO_0004282
DR   MCCL; MCC:0000212
DR   CLDB; cl1745
DR   ATCC; CRL-1841
DR   ECACC; 92021401
DR   Wikidata; Q54896508
RX   PubMed=31220119;
CC   Problematic cell line: Probably misidentified/contaminated. While said to originate from a BALB/c mouse it exhibits a greater similarity with a C57BL strain. Its STR profile has three alleles or more at seven loci, high peak height imbalance and is discordant at eight STR markers. It therefore needs more characterization to determine if it is contaminated or misidentified due to the inconsistencies with the strain of origin and the increase of alleles per marker (PubMed=31220119).
CC   Characteristics: IL2 and IL4 dependent.
CC   Breed/subspecies: BALB/c.
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_5T92 ! HT-2
CA   Factor-dependent cell line
DT   Created: 04-04-12; Last updated: 21-03-23; Version: 9
//
RX   PubMed=31220119; DOI=10.1371/journal.pone.0218412; PMCID=PMC6586308;
RA   Almeida, Jamie L.
RA   Dakic, Aleksandra
RA   Kindig, Karin
RA   Kone, Maikan
RA   Letham, Deborah Lee Dormady
RA   Langdon, Scott
RA   Peat, Ruth
RA   Holding-Pillai, Jayamalini
RA   Hall, Erin M.
RA   Ladd, Mark
RA   Shaffer, Megan D.
RA   Berg, Heath
RA   Li, Jin-Liang
RA   Wigger, Georges
RA   Lund, Steve
RA   Steffen, Carolyn R.
RA   Fransway, Barbara B.
RA   Geraghty, Bob
RA   Natoli, Manuela
RA   Bauer, Beth A.
RA   Gollin, Susanne M.
RA   Lewis, Dale W.
RA   Reid, Yvonne A.
RT   "Interlaboratory study to validate a STR profiling method for
RT   intraspecies identification of mouse cell lines.";
RL   PLoS ONE 14:e0218412.1-e0218412.24(2019).
//