Cellosaurus cell line HIT-T15 (CVCL

Cellosaurus HIT-T15 (CVCL_0301)

Cross-references
Cell line name	HIT-T15
Synonyms	HIT T15; HIT.T15; HIT T-15; HITT15; HIT clone T15; Hamster Islet Transformed-Tioguanine resistant clone 15; HIT
Accession	CVCL_0301
Resource Identification Initiative	To cite this cell line use: HIT-T15 (RRID:CVCL_0301)
Comments	Characteristics: Secretes insulin. Maximal stimulation is obtained with glucose, arginine and leucine (PubMed=3535793). Doubling time: 33-34 hours (PubMed=6270673). Selected for resistance to: ChEBI; CHEBI:9555; Tioguanine (6-thioguanine; 6-TG). Transformant: ChEBI; CHEBI:23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE). Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40). Derived from site: In situ; Pancreas, islets of Langerhans; UBERON=UBERON_0000006.
Species of origin	Mesocricetus auratus (Golden hamster) (NCBI Taxonomy: 10036)
Sex of cell	Sex unspecified
Age at sampling	Age unspecified
Category	Transformed cell line
Web pages	https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/h/cell-lines-detail-502.html
Publications	PubMed=6270673; DOI=10.1073/pnas.78.7.4339; PMCID=PMC319785 Santerre R.F., Cook R.A., Crisel R.M.D., Sharp J.D., Schmidt R.J., Williams D.C., Wilson C.P. Insulin synthesis in a clonal cell line of simian virus 40-transformed hamster pancreatic beta cells. Proc. Natl. Acad. Sci. U.S.A. 78:4339-4343(1981) PubMed=3535793; DOI=10.1016/0006-291X(86)90776-X Lambert D.G., Hughes K., Atkins T.W. Insulin release from a cloned hamster B-cell line (HIT-T15). The effects of glucose, amino acids, sulphonylureas and colchicine. Biochem. Biophys. Res. Commun. 140:616-625(1986) PubMed=2569024; DOI=10.1677/joe.0.1210479 Lambert D.G., Atkins T.W. Effects of islet hormones on insulin secretion from cloned B cell lines, HIT-T15 and RINm5F. J. Endocrinol. 121:479-485(1989) PubMed=8697299 Poitout V., Olson L.K., Robertson R.P. Insulin-secreting cell lines: classification, characteristics and potential applications. Diabetes Metab. 22:7-14(1996)
Cell line collections (Providers)	ATCC; CRL-1777 IZSLER; BS TCL 188 KCB; KCB 200828YJ KCLB; 21777
Cell line databases/resources	CLO; CLO_0003758 MCCL; MCC:0000192
Anatomy/cell type resources	BTO; BTO:0000627
Encyclopedic resources	Wikidata; Q54889679
Entry history
Entry creation	04-Apr-2012
Last entry update	02-May-2024
Version number	18