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Cellosaurus HIT-T15 (CVCL_0301)

[Text version]

Cell line name HIT-T15
Synonyms HIT T15; HIT.T15; HIT T-15; HITT15; HIT clone T15; Hamster Islet Transformed-Tioguanine resistant clone 15; HIT
Accession CVCL_0301
Resource Identification Initiative To cite this cell line use: HIT-T15 (RRID:CVCL_0301)
Comments Characteristics: Secretes insulin. Maximal stimulation is obtained with glucose, arginine and leucine (PubMed=3535793).
Doubling time: 33-34 hours (PubMed=6270673).
Selected for resistance to: ChEBI; CHEBI:9555; Tioguanine (6-thioguanine; 6-TG).
Transformant: ChEBI; CHEBI:23994; Ethyl methanesulfonate (Methylsulfonic acid ethyl ester; MSEE).
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40).
Derived from sampling site: Pancreas; islets of Langerhans.
Species of origin Mesocricetus auratus (Golden hamster) (NCBI Taxonomy: 10036)
Sex of cell Sex unspecified
Age at sampling Age unspecified
Category Transformed cell line
Web pages https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/h/cell-lines-detail-502.html

PubMed=6270673; DOI=10.1073/pnas.78.7.4339
Santerre R.F., Cook R.A., Crisel R.M., Sharp J.D., Schmidt R.J., Williams D.C., Wilson C.P.
Insulin synthesis in a clonal cell line of simian virus 40-transformed hamster pancreatic beta cells.
Proc. Natl. Acad. Sci. U.S.A. 78:4339-4343(1981)

PubMed=3535793; DOI=10.1016/0006-291X(86)90776-X
Lambert D.G., Hughes K., Atkins T.W.
Insulin release from a cloned hamster B-cell line (HIT-T15). The effects of glucose, amino acids, sulphonylureas and colchicine.
Biochem. Biophys. Res. Commun. 140:616-625(1986)

Poitout V., Olson L.K., Robertson R.P.
Insulin-secreting cell lines: classification, characteristics and potential applications.
Diabetes Metab. 22:7-14(1996)

Cell line collections ATCC; CRL-1777
KCB; KCB 200828YJ
KCLB; 21777
Cell line databases/resources CLO; CLO_0003758
MCCL; MCC:0000192
Anatomy/cell type resources BTO; BTO:0000627
Encyclopedic resources Wikidata; Q54889679
Entry history
Entry creation04-Apr-2012
Last entry update20-May-2021
Version number15