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Cellosaurus HCA7 (CVCL_0289)

[Text version]
Cell line name HCA7
Synonyms HCA-7; HCA 7
Accession CVCL_0289
Resource Identification Initiative To cite this cell line use: HCA7 (RRID:CVCL_0289)
Comments Part of: KuDOS 95 cell line panel.
Microsatellite instability: Instable (MSI) (PubMed=24755471; PubMed=25926053).
Omics: Proteomics.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Colon; UBERON=UBERON_0001155.
Sequence variations
  • Mutation; HGNC; HGNC:583; APC; Simple; p.Glu582fs*20 (c.1742_1743insA); Zygosity=Unspecified (PubMed=24755471).
  • Mutation; HGNC; HGNC:583; APC; Simple; p.Thr1556Asnfs*3 (c.4666dupA) (c.4666_4667insA); ClinVar=VCV000428112; Zygosity=Unspecified (PubMed=24755471).
  • Mutation; HGNC; HGNC:16712; FBXW7; Simple; p.Arg479Ter (c.1435C>T); Zygosity=Unspecified (PubMed=24755471).
  • Mutation; HGNC; HGNC:11998; TP53; Simple; p.Pro301Glnfs*44 (c.898delC); Zygosity=Unspecified (PubMed=16418264; PubMed=24755471).
HLA typing Source: PubMed=26589293
Class I
HLA-AA*01:01,02:01
HLA-BB*37:01,51:01
HLA-CC*06:02,14:02
Disease Colon adenocarcinoma (NCIt: C4349)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_2469 (HCA7 colony 1)CVCL_2470 (HCA7 colony 11)CVCL_2471 (HCA7 colony 24)
CVCL_2472 (HCA7 colony 26)CVCL_2473 (HCA7 colony 27)CVCL_2474 (HCA7 colony 29)
CVCL_2475 (HCA7 colony 3)CVCL_2476 (HCA7 colony 30)CVCL_2477 (HCA7 colony 6)
CVCL_D8GV (Ubigene HCA-7 RNF43 KO)
Sex of cell Female
Age at sampling 58Y
Category Cancer cell line
STR profile Source(s): AddexBio=C0009003/40; ECACC=06061902; PubMed=25877200; PubMed=25926053

Markers:
AmelogeninX
CSF1PO11,14
D3S135815,19
D5S8188,12 (ECACC=06061902; PubMed=25877200)
9,11,12 (PubMed=25926053)
9,12 (AddexBio=C0009003/40)
D7S82011,12
D8S117912,14
D13S3179
D16S53910,11 (AddexBio=C0009003/40; ECACC=06061902; PubMed=25926053)
11 (PubMed=25877200)
D18S5119
D21S1126,28.2 (PubMed=25877200)
26,28.2,29.2 (PubMed=25926053)
FGA18.2,22
Penta D9
Penta E12,18
TH016,7
TPOX8,10
vWA14,16

Run an STR similarity search on this cell line
Web pages Info; Pawefish; -; https://www.cellosaurus.org/pawefish/ColonCellLineDescriptions/HCA7.html
Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/h/cell-lines-detail-584.html
Publications

PubMed=4016751
Susan C. Kirkland;
Dome formation by a human colonic adenocarcinoma cell line (HCA-7).
Cancer Res. 45:3790-3795(1985)

PubMed=3718830; DOI=10.1038/bjc.1986.132; PMCID=PMC2001395
Susan C. Kirkland, I.G. Bailey;
Establishment and characterisation of six human colorectal adenocarcinoma cell lines.
Br. J. Cancer 53:779-785(1986)

PubMed=8410493; DOI=10.1002/path.1711700407
Katrina A. Marsh, Gordon W.H. Stamp, Susan C. Kirkland;
Isolation and characterization of multiple cell types from a single human colonic carcinoma: tumourigenicity of these cell types in a xenograft system.
J. Pathol. 170:441-450(1993)

PubMed=8464898; DOI=10.1073/pnas.90.7.2842; PMCID=PMC46192
Michael J. Browning, Peter Krausa, Andrew J. Rowan, David C. Bicknell, Julia Gwynaeth Bodmer, Walter Fred Bodmer;
Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines.
Proc. Natl. Acad. Sci. U.S.A. 90:2842-2845(1993)

PubMed=9294210; DOI=10.1073/pnas.94.19.10330; PMCID=PMC23362
Mohammad Ilyas, Ian Philip Mark Tomlinson, Andrew J. Rowan, Massimo Pignatelli, Walter Fred Bodmer;
Beta-catenin mutations in cell lines established from human colorectal cancers.
Proc. Natl. Acad. Sci. U.S.A. 94:10330-10334(1997)

PubMed=10737795; DOI=10.1073/pnas.97.7.3352; PMCID=PMC16243
Andrew J. Rowan, Hanan Lamlum, Mohammad Ilyas, James M.D. Wheeler, Jannes Straub, Angeliki Papadopoulou, David C. Bicknell, Walter Fred Bodmer, Ian Philip Mark Tomlinson;
APC mutations in sporadic colorectal tumors: a mutational 'hotspot' and interdependence of the 'two hits'.
Proc. Natl. Acad. Sci. U.S.A. 97:3352-3357(2000)

PubMed=11226274; DOI=10.1073/pnas.041603298; PMCID=PMC30173
Wael M. Abdel-Rahman, Kanade Katsura, Willem Rens, Patricia A. Gorman, Denise Sheer, David C. Bicknell, Walter Fred Bodmer, Mark J. Arends, Andrew Hamilton Wyllie, Paul A.W. Edwards;
Spectral karyotyping suggests additional subsets of colorectal cancers characterized by pattern of chromosome rearrangement.
Proc. Natl. Acad. Sci. U.S.A. 98:2538-2543(2001)

PubMed=16418264; DOI=10.1073/pnas.0510146103; PMCID=PMC1327731
Ying Liu, Walter Fred Bodmer;
Analysis of p53 mutations and their expression in 56 colorectal cancer cell lines.
Proc. Natl. Acad. Sci. U.S.A. 103:976-981(2006)

PubMed=18258742; DOI=10.1073/pnas.0712176105; PMCID=PMC2268141
Muhammad Emaduddin, David C. Bicknell, Walter Fred Bodmer, Stephan M. Feller;
Cell growth, global phosphotyrosine elevation, and c-Met phosphorylation through Src family kinases in colorectal cancer cells.
Proc. Natl. Acad. Sci. U.S.A. 105:2358-2362(2008)

PubMed=20570890; DOI=10.1158/0008-5472.CAN-10-0192; PMCID=PMC2943514
Manickam Janakiraman, Efsevia Vakiani, Zhao-Shi Zeng, Christine A. Pratilas, Barry Stephen Taylor, Dhananjay Chitale, Ensar Halilovic, Manda Wilson, Kety Huberman, Julio Cezar Marques Ricarte Filho, Yogindra Persaud ...Show all 21 authors... , Douglas A. Levine, James Alexander Fagin, Suresh C. Jhanwar, John M. Mariadason, Alex Lash, Marc Ladanyi, Leonard B. Saltz, Adriana Heguy, Philip B. Paty, David B. Solit; Show fewer authors
Genomic and biological characterization of exon 4 KRAS mutations in human cancer.
Cancer Res. 70:5901-5911(2010)

PubMed=20606684; DOI=10.1038/sj.bjc.6605780; PMCID=PMC2920028
Karin Bracht, Angela M. Nicholls, Ying Liu, Walter Fred Bodmer;
5-fluorouracil response in a large panel of colorectal cancer cell lines is associated with mismatch repair deficiency.
Br. J. Cancer 103:340-346(2010)

PubMed=24755471; DOI=10.1158/0008-5472.CAN-14-0013
Dmitri Mouradov, Clare Sloggett, Robert N. Jorissen, Christopher G. Love, Shan Li, Antony Wilks Burgess, Diego Arango, Robert L. Strausberg, Daniel Buchanan, Samuel Wormald, Liam O'Connor ...Show all 17 authors... , Jennifer L. Wilding, David C. Bicknell, Ian Philip Mark Tomlinson, Walter Fred Bodmer, John M. Mariadason, Oliver M. Sieber; Show fewer authors
Colorectal cancer cell lines are representative models of the main molecular subtypes of primary cancer.
Cancer Res. 74:3238-3247(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Christiaan Klijn, Steffen Durinck, Eric W. Stawiski, Peter M. Haverty, Zhao-Shi Jiang, Han-Bin Liu, Jeremiah Degenhardt, Oleg Mayba, Florian Gnad, Jin-Feng Liu, Gregoire Pau ...Show all 30 authors... , Jens Reeder, Yi Cao, Kiran Mukhyala, Suresh K. Selvaraj, Ma-Mie Yu, Gregory J. Zynda, Matthew J. Brauer, Thomas D. Wu, Robert Clifford Gentleman, Gerard Manning, Robert L. Yauch, Richard Bourgon, David Stokoe, Zora Modrusan, Richard M. Neve, Frederic J. de Sauvage, Jeffrey Settleman, Somasekar Seshagiri, Ze-Min Zhang; Show fewer authors
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=25926053; DOI=10.1038/ncomms8002
Enzo Medico, Mariangela Russo, Gabriele Picco, Carlotta Cancelliere, Emanuele Valtorta, Giorgio Corti, Michela Buscarino, Claudio Isella, Simona Lamba, Barbara Martinoglio, Silvio Veronese ...Show all 19 authors... , Salvatore Siena, Andrea Sartore-Bianchi, Marco Beccuti, Marcella Mottolese, Michael Linnebacher, Francesca Cordero, Federica Di Nicolantonio, Alberto Bardelli; Show fewer authors
The molecular landscape of colorectal cancer cell lines unveils clinically actionable kinase targets.
Nat. Commun. 6:7002.1-7002.10(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Jelle Scholtalbers, Sebastian Boegel, Thomas Bukur, Marius Byl, Sebastian Goerges, Patrick Sorn, Martin Loewer, Ugur Sahin, John C. Castle;
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=29101300; DOI=10.15252/msb.20177701; PMCID=PMC5731344
Martin Frejno, Riccardo Zenezini Chiozzi, Mathias Wilhelm, Heiner Koch, Run-Sheng Zheng, Susan Klaeger, Benjamin Ruprecht, Chen Meng, Karl Kramer, Anna Jarzab, Stephanie Heinzlmeir ...Show all 20 authors... , Elaine Johnstone, Enric Domingo, David James Kerr, Moritz Jesinghaus, Julia Slotta-Huspenina, Wilko Weichert, Stefan Knapp, Stephan M. Feller, Bernhard Kuster; Show fewer authors
Pharmacoproteomic characterisation of human colon and rectal cancer.
Mol. Syst. Biol. 13:951-951(2017)

Cross-references
Cell line collections (Providers) AddexBio; C0009003/40
ECACC; 06061902
Ximbio; 152451 - Discontinued
Cell line databases/resources MCCL; MCC:0000179
cancercelllines; CVCL_0289
ColonAtlas; HCA7
Anatomy/cell type resources BTO; BTO_0003031
Biological sample resources BioSample; SAMN03471988
Chemistry resources PharmacoDB; HCA7_445_2019
Encyclopedic resources Wikidata; Q54881491
Experimental variables resources EFO; EFO_0006415
Gene expression databases ArrayExpress; E-MTAB-2706
GEO; GSM741260
GEO; GSM1346872
GEO; GSM1374491
GEO; GSM1374492
GEO; GSM1448125
GEO; GSM3145609
GEO; GSM3145708
Polymorphism and mutation databases Cosmic; 876722
Cosmic; 948860
Cosmic; 985995
Cosmic; 995407
Cosmic; 1043813
Cosmic; 1223140
Cosmic; 1312301
Cosmic; 1479629
Cosmic; 1486131
Cosmic; 1805252
Cosmic; 1933017
Cosmic; 2301976
Cosmic; 2760084
IARC_TP53; 21760
Proteomic databases PRIDE; PXD005354
PRIDE; PXD005355
Sequence databases EGA; EGAS00001000610
Entry history
Entry creation04-Apr-2012
Last entry update14-Aug-2025
Version number39