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Cellosaurus Chang Liver (CVCL_0238)

[Text version]
Cell line name Chang Liver
Synonyms Chang liver; Chang Liver (HeLa derivative); Chang Cells; Chang; CHL
Accession CVCL_0238
Resource Identification Initiative To cite this cell line use: Chang Liver (RRID:CVCL_0238)
Comments Problematic cell line: Contaminated. Shown to be a HeLa derivative (PubMed=4864103; PubMed=5641128; PubMed=1246601; PubMed=20143388; PubMed=21656536). Originally thought to originate from normal hepatic cells obtained from a liver biopsy of an adult.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00002.
Population: African American.
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Omics: Genomics; Whole genome sequencing; Low read coverage.
Omics: Proteomics; PTM; Ubiquitination.
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related endocervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0030 (HeLa)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
STR profile Source(s): ATCC=CCL-13; CLS=300139; ECACC=88021102; JCRB=IFO50016; JCRB=JCRB9066; PubMed=11416159

Markers:
AmelogeninX
CSF1PO9,10 (JCRB=IFO50016; JCRB=JCRB9066)
10 (ATCC=CCL-13; CLS=300139; ECACC=88021102)
D2S133817,18
D3S135815,18
D5S81811,12 (JCRB=IFO50016; JCRB=JCRB9066)
12 (ATCC=CCL-13; CLS=300139; ECACC=88021102)
D7S8208,12
D8S117912,13
D13S31712,13.3 (ATCC=CCL-13; CLS=300139; JCRB=JCRB9066)
12,14 (ECACC=88021102; JCRB=IFO50016)
D16S5399,10
D18S5116
D19S43313,14
D21S1127,28
FGA21
Penta D8,15
Penta E7,17
TH017
TPOX8,12
vWA16,18

Run an STR similarity search on this cell line
Web pages Info; ICLAC; -; https://iclac.org/education/chang-liver/
Info; ICLAC; -; https://iclac.org/wp-content/uploads/Cross-Contaminations_v13_distribution.xlsx
Publications

PubMed=13472685
Jerome Theda Syverton, Leroy Clarence McLaren Jr.;
Human cells in continuous culture. I. Derivation of cell strains from esophagus, palate, liver, and lung.
Cancer Res. 17:923-926(1957)

PubMed=13531635; DOI=10.1007/BF00340146
Charles Marc Pomerat, Sidney P. Kent, Loren C. Logie;
Irradiation of cells in tissue culture. I. Giant cell induction in strain cultures versus elements from primary explants.
Z. Zellforsch. Mikrosk. Anat. 47:158-174(1957)

PubMed=14185313; DOI=10.1126/science.146.3641.241
Cell Culture Collection Committee
Animal cell strains. The Cell Culture Collection Committee has assembled and certified 23 strains of animal cells.
Science 146:241-243(1964)

PubMed=4864103
Stanley Michael Gartler;
Genetic markers as tracers in cell culture.
Natl. Cancer Inst. Monogr. 26:167-195(1967)

PubMed=5641128; DOI=10.1038/217750a0
Stanley Michael Gartler;
Apparent Hela cell contamination of human heteroploid cell lines.
Nature 217:750-751(1968)

DOI=10.1007/BF02618370
Cyril S. Stulberg, Lewis Lemon Coriell, Alexis John Kniazeff, John E. Shannon;
The animal cell culture collection.
In Vitro 5:1-16(1970)

DOI=10.1007/978-1-4757-1647-4_13
June Lee Biedler;
Chromosome abnormalities in human tumor cells in culture.
(In book chapter) Human tumor cells in vitro; Fogh, Jorgen (eds.); pp.359-394; Springer; New York; USA (1975)

PubMed=1246601; DOI=10.1126/science.1246601
Walter Anthony Nelson-Rees, Robert R. Flandermeyer;
HeLa cultures defined.
Science 191:96-98(1976)

PubMed=468301; PMCID=PMC1457289
Ludwik K. Trejdosiewicz, Avril J. Trejdosiewicz, Noel Russell Ling, Peter W. Dykes;
Growth enhancing property of human monocytes from normal donors and cancer patients.
Immunology 37:247-252(1979)

PubMed=6988327; DOI=10.1007/BF02831503
Stephen James O'Brien, John E. Shannon, Mitchell H. Gail;
A molecular approach to the identification and individualization of human and animal cells in culture: isozyme and allozyme genetic signatures.
In Vitro 16:119-135(1980)

CLPUB00262
Michael Gold;
A conspiracy of cells. One woman's immortal legacy and the medical scandal it caused.
(In book) ISBN 9780887060991; pp.1-171; State University of New York Press; Albany; USA (1986)

PubMed=11416159; DOI=10.1073/pnas.121616198; PMCID=PMC35459
John R.W. Masters, Jim A. Thomson, Bernadette Daly-Burns, Yvonne A. Reid, Wilhelm Gerhard Dirks, Phil Packer, Lorraine H. Toji, Tadao Ohno, Hideyuki Tanabe, Colin Francis Arlett, Lloyd R. Kelland ...Show all 16 authors... , Maureen Harrison, Arvind K. Virmani, Timothy H. Ward, Karen L. Ayres, Paul G. Debenham; Show fewer authors
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

PubMed=18655026; DOI=10.1002/pmic.200700887
Feng-Wei Tan, Li-Fang Lu, Yun Cai, Jing-Lan Wang, Yun-Fei Xie, Lin Wang, Yan-Hua Gong, Bing-E. Xu, Jun Wu, Ying Luo, Bo-Qin Qiang ...Show all 14 authors... , Jian-Gang Yuan, Xiao-Qing Sun, Xiao-Zhong Peng; Show fewer authors
Proteomic analysis of ubiquitinated proteins in normal hepatocyte cell line Chang liver cells.
Proteomics 8:2885-2896(2008)

PubMed=20143388; DOI=10.1002/ijc.25242
Amanda Capes-Davis, George Theodosopoulos, Isobel Atkin, Hans Gunther Drexler, Arihiro Kohara, Roderick A.F. MacLeod, John R.W. Masters, Yukio Nakamura, Yvonne A. Reid, Roger Robert Reddel, Robert Ian Freshney;
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=21656536; DOI=10.1002/hep.24475
Qiang Gao, Xiao-Ying Wang, Jian Zhou, Jia Fan;
Cell line misidentification: the case of the Chang liver cell line.
Hepatology 54:1894-1895(2011)

PubMed=23925245; DOI=10.1038/nature12064; PMCID=PMC3740412
Andrew C. Adey, Joshua N. Burton, Jacob O. Kitzman, Joseph B. Hiatt, Alexandra P. Lewis, Beth K. Martin, Ruo-Lan Qiu, Choli Lee, Jay Shendure;
The haplotype-resolved genome and epigenome of the aneuploid HeLa cancer cell line.
Nature 500:207-211(2013)

Cross-references
Cell line collections (Providers) ATCC; CCL-13
BCRC; 60024 - Discontinued
CLS; 300139
ECACC; 88021102
IZSLER; BS CL 14
JCRB; IFO50016
JCRB; JCRB9066
RCB; RCB0491 - Discontinued
Cell line databases/resources CLO; CLO_0002414
CLO; CLO_0002415
MCCL; MCC:0000128
CLDB; cl715
CLDB; cl716
CLDB; cl717
CLDB; cl718
TOKU-E; 937
Anatomy/cell type resources BTO; BTO_0002049
Biological sample resources BioSample; SAMN03151672
BioSample; SAMN03472417
BioSample; SAMN03472705
Encyclopedic resources Wikidata; Q54811726
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number37