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Cellosaurus Chang Liver (CVCL_0238)

[Text version]
Cell line name Chang Liver
Synonyms Chang liver; Chang Liver (HeLa derivative); Chang Cells; Chang; CHL
Accession CVCL_0238
Resource Identification Initiative To cite this cell line use: Chang Liver (RRID:CVCL_0238)
Comments Problematic cell line: Contaminated. Shown to be a HeLa derivative (PubMed=4864103; PubMed=5641128; PubMed=1246601; PubMed=20143388; PubMed=21656536). Originally thought to originate from normal hepatic cells obtained from a liver biopsy of an adult.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00002.
Population: African American.
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Omics: Genome sequenced (low read coverage).
Omics: Ubiquitination analysis by proteomics.
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related cervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0030 (HeLa)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
STR profile Source(s): ATCC; CLS; ECACC; JCRB; PubMed=11416159

D5S81811,12 (JCRB)
D13S31712,13.3 (ATCC; CLS; JCRB)
12,14 (ECACC)
Penta D8,15
Penta E7,17
vWA16,17,18 (JCRB)
16,18 (ATCC; CLS; ECACC; PubMed=11416159)

Run an STR similarity search on this cell line
Web pages https://iclac.org/education/chang-liver/

Syverton J.T., McLaren L.C.
Human cells in continuous culture. I. Derivation of cell strains from esophagus, palate, liver, and lung.
Cancer Res. 17:923-926(1957)

PubMed=13531635; DOI=10.1007/BF00340146
Pomerat C.M., Kent S.P., Logie L.C.
Irradiation of cells in tissue culture. I. Giant cell induction in strain cultures versus elements from primary explants.
Z. Zellforsch. Mikrosk. Anat. 47:158-174(1957)

PubMed=14185313; DOI=10.1126/science.146.3641.241
Cell Culture Collection Committee
Animal cell strains. The Cell Culture Collection Committee has assembled and certified 23 strains of animal cells.
Science 146:241-243(1964)

Gartler S.M.
Genetic markers as tracers in cell culture.
Natl. Cancer Inst. Monogr. 26:167-195(1967)

PubMed=5641128; DOI=10.1038/217750a0
Gartler S.M.
Apparent Hela cell contamination of human heteroploid cell lines.
Nature 217:750-751(1968)

Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.
The animal cell culture collection.
In Vitro 5:1-16(1970)

Biedler J.L.
Chromosome abnormalities in human tumor cells in culture.
(In) Human tumor cells in vitro; Fogh J. (eds.); pp.359-394; Springer; New York (1975)

PubMed=1246601; DOI=10.1126/science.1246601
Nelson-Rees W.A., Flandermeyer R.R.
HeLa cultures defined.
Science 191:96-98(1976)

Trejdosiewicz L.K., Trejdosiewicz A.J., Ling N.R., Dykes P.W.
Growth enhancing property of human monocytes from normal donors and cancer patients.
Immunology 37:247-252(1979)

PubMed=6988327; DOI=10.1007/BF02831503
O'Brien S.J., Shannon J.E., Gail M.H.
A molecular approach to the identification and individualization of human and animal cells in culture: isozyme and allozyme genetic signatures.
In Vitro 16:119-135(1980)

Gold M.
A conspiracy of cells. One woman's immortal legacy and the medical scandal it caused.
(In) ISBN 9780887060991; pp.1-171; State University of New York Press; Albany (1986)

PubMed=11416159; DOI=10.1073/pnas.121616198
Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G., Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R., Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

PubMed=18655026; DOI=10.1002/pmic.200700887
Tan F.-W., Lu L.-F., Cai Y., Wang J.-L., Xie Y.-F., Wang L., Gong Y.-H., Xu B.-E., Wu J., Luo Y., Qiang B.-Q., Yuan J.-G., Sun X.-Q., Peng X.-Z.
Proteomic analysis of ubiquitinated proteins in normal hepatocyte cell line Chang liver cells.
Proteomics 8:2885-2896(2008)

PubMed=20143388; DOI=10.1002/ijc.25242
Capes-Davis A., Theodosopoulos G., Atkin I., Drexler H.G., Kohara A., MacLeod R.A.F., Masters J.R.W., Nakamura Y., Reid Y.A., Reddel R.R., Freshney R.I.
Check your cultures! A list of cross-contaminated or misidentified cell lines.
Int. J. Cancer 127:1-8(2010)

PubMed=21656536; DOI=10.1002/hep.24475
Gao Q., Wang X.-Y., Zhou J., Fan J.
Cell line misidentification: the case of the Chang liver cell line.
Hepatology 54:1894-1895(2011)

PubMed=23925245; DOI=10.1038/nature12064
Adey A., Burton J.N., Kitzman J.O., Hiatt J.B., Lewis A.P., Martin B.K., Qiu R.-L., Lee C., Shendure J.
The haplotype-resolved genome and epigenome of the aneuploid HeLa cancer cell line.
Nature 500:207-211(2013)

Cell line collections (Providers) ATCC; CCL-13
BCRC; 60024 - Discontinued
CLS; 300139
ECACC; 88021102
JCRB; IFO50016
RCB; RCB0491 - Discontinued
Cell line databases/resources CLO; CLO_0002414
CLO; CLO_0002415
MCCL; MCC:0000128
CLDB; cl715
CLDB; cl716
CLDB; cl717
CLDB; cl718
TOKU-E; 937
Anatomy/cell type resources BTO; BTO:0002049
Biological sample resources BioSample; SAMN03151672
BioSample; SAMN03472417
BioSample; SAMN03472705
Encyclopedic resources Wikidata; Q54811726
Entry history
Entry creation04-Apr-2012
Last entry update05-Oct-2023
Version number34