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Cellosaurus Caki-2 (CVCL_0235)

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Cell line name Caki-2
Synonyms CAKI-2; CaKi-2; caki-2; CAKI 2; Caki 2; Caki2; CAKI2
Accession CVCL_0235
Resource Identification Initiative To cite this cell line use: Caki-2 (RRID:CVCL_0235)
Comments Problematic cell line: Misclassified. Originally thought to be a clear cell renal cell carcinoma but shown to be from a papillary renal cell carcinoma (PubMed=17409424; PubMed=27993170).
Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: MD Anderson Cell Lines Project.
From: Memorial Sloan Kettering Cancer Center; New York; USA.
Registration: Memorial Sloan Kettering Cancer Center Office of Technology Development; SK2010-068.
Population: Caucasian.
Doubling time: ~30-40 hours (DSMZ=ACC-54).
Omics: Genomics; Whole exome sequencing.
Omics: Proteomics; Expression; Reverse-phase protein array.
Omics: Proteomics; PTM; GPI-anchored proteins.
Omics: Proteomics; Quantitative.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; Array-based CGH.
Omics: Variations; SNP array analysis.
Derived from site: In situ; Kidney; UBERON=UBERON_0002113.
HLA typing Source: PubMed=15585611
Class I
HLA-AA*01,11
HLA-BB*08,51
HLA-CC*07,12

Source: PubMed=26589293
Class I
HLA-AA*01:01,11:01
HLA-BB*08:01,52:01
HLA-CC*07:01,12:02
Genome ancestry Source: PubMed=30894373

Origin% genome
African0
Native American0.61
East Asian, North1.28
East Asian, South0
South Asian0
European, North64.34
European, South33.76
Disease Papillary renal cell carcinoma (NCIt: C6975)
Papillary renal cell carcinoma (ORDO: Orphanet_319298)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_E3VW (Caki-2 DNMT3A KO)
Sex of cell Male
Age at sampling 69Y
Category Cancer cell line
STR profile Source(s): ATCC=HTB-47; CCRID=1101HUM-PUMC000337; CLS=300140; COG; DSMZ=ACC-54; ECACC=93120819; KCLB=30047; PubMed=25877200; Technion Genomics Center

Markers:
AmelogeninX,Y
CSF1PO10,12
D1S165612,14
D2S44114
D2S133817,20
D3S135814
D5S81811
D7S82012
D8S117910
D10S124814,16
D12S39119,23
D13S31710
D16S5399,13
D18S5117
D19S43313,14
D21S1127,31
D22S104515
FGA22
Penta D10,13
Penta E7,17
TH016
TPOX9,11
vWA16,17

Run an STR similarity search on this cell line
Web pages Info; MCLP; -; https://tcpaportal.org/mclp/
Provider; MSKCC; -; https://www.mskcc.org/research-advantage/support/technology/tangible-material/caki-2-human-renal-cell-line
Publications

DOI=10.1007/978-1-4757-1647-4_5
Jorgen Fogh, Germain L. Trempe;
New human tumor cell lines.
(In book chapter) Human tumor cells in vitro; Fogh, Jorgen (eds.); pp.115-159; Springer; New York; USA (1975)

PubMed=327080; DOI=10.1093/jnci/59.1.221
Jorgen Fogh, Jens M. Fogh, Thomas Orfeo;
One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice.
J. Natl. Cancer Inst. 59:221-226(1977)

PubMed=833871; DOI=10.1093/jnci/58.2.209
Jorgen Fogh, William C. Wright, James D. Loveless;
Absence of HeLa cell contamination in 169 cell lines derived from human tumors.
J. Natl. Cancer Inst. 58:209-214(1977)

PubMed=571047
Jorgen Fogh;
Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors.
Natl. Cancer Inst. Monogr. 49:5-9(1978)

PubMed=6244232
Richard D. Williams;
Human urologic cancer cell lines.
Invest. Urol. 17:359-363(1980)

PubMed=6935474; DOI=10.1093/jnci/66.2.239
William C. Wright, Walter P. Daniels, Jorgen Fogh;
Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis.
J. Natl. Cancer Inst. 66:239-247(1981)

PubMed=7459858
Monique Rousset, Alain Zweibaum, Jorgen Fogh;
Presence of glycogen and growth-related variations in 58 cultured human tumor cell lines of various tissue origins.
Cancer Res. 41:1165-1170(1981)

PubMed=6582512; DOI=10.1073/pnas.81.2.568; PMCID=PMC344720
Michael Jules Mattes, Carlos Cordon-Cardo, John L. Lewis Jr., Lloyd John Old, Kenneth Oliver Lloyd;
Cell surface antigens of human ovarian and endometrial carcinoma defined by mouse monoclonal antibodies.
Proc. Natl. Acad. Sci. U.S.A. 81:568-572(1984)

PubMed=3518877; DOI=10.3109/07357908609038260
Jorgen Fogh;
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)

PubMed=7591954; DOI=10.1111/j.1349-7006.1995.tb03087.x; PMCID=PMC5920923
Hidefumi Kinoshita, Hitoshi Yamada, Osamu Ogawa, Yoshiyuki Kakehi, Mitsuhiko Osaka, Eijiro Nakamura, Mutsuki Mishina, Tomonori Habuchi, Rei Takahashi, Taketoshi Sugiyama, Osamu Yoshida;
Contribution of chromosome 9p21-22 deletion to the progression of human renal cell carcinoma.
Jpn. J. Cancer Res. 86:795-799(1995)

PubMed=10723130; DOI=10.1038/sj.onc.1203449
Andrei Alimov, Maria Kost-Alimova, Jian Liu, Chun-De Li, Ulf S.R. Bergerheim, Stefan Imreh, George Klein, Eugene R. Zabarovsky;
Combined LOH/CGH analysis proves the existence of interstitial 3p deletions in renal cell carcinoma.
Oncogene 19:1392-1399(2000)

PubMed=10929426; DOI=10.1007/s002400000103
Ichiro Shintaku, Nobutoshi Kawagoe, Shigeru Yutani, Sakuo Hoshi, Seiichi Orikasa, Osamu Yoshizumi, Kyogo Itoh;
Expression of the SART1 tumor rejection antigen in renal cell carcinoma.
Urol. Res. 28:178-184(2000)

PubMed=15585611; DOI=10.1158/1078-0432.CCR-04-0072
Scott Simon Tykodi, Edus Houston Warren, John A. Thompson, Stanley R. Riddell, Richard W. Childs, Brith E. Otterud, Mark F. Leppert, Rainer Storb, Brenda M. Sandmaier;
Allogeneic hematopoietic cell transplantation for metastatic renal cell carcinoma after nonmyeloablative conditioning: toxicity, clinical response, and immunological response to minor histocompatibility antigens.
Clin. Cancer Res. 10:7799-7811(2004)

PubMed=17409424; DOI=10.1158/0008-5472.CAN-06-4571
Kyle A. Furge, Jin-Dong Chen, Julie Koeman, Pamela J. Swiatek, Karl Dykema, Ksenija Lucin, Richard Kahnoski, Xi-Ming J. Yang, Bin Tean Teh;
Detection of DNA copy number changes and oncogenic signaling abnormalities from gene expression data reveals MYC activation in high-grade papillary renal cell carcinoma.
Cancer Res. 67:3171-3176(2007)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458; PMCID=PMC2881662
S. Michael Rothenberg, Gayatry Mohapatra, Miguel N. Rivera, Daniel Winokur, Patricia Greninger, Mai Nitta, Peter M. Sadow, Gaya Sooriyakumar, Brian W. Brannigan, Matthew J. Ulman, Rushika M. Perera ...Show all 22 authors... , Rui Wang, Angela Tam, Xiao-Jun Ma, Mark Erlander, Dennis C. Sgroi, James W. Rocco, Mark W. Lingen, Ezra E.W. Cohen, David Neil Louis, Jeffrey Settleman, Daniel Arie Haber; Show fewer authors
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=22185343; DOI=10.1186/1471-2407-11-523; PMCID=PMC3292516
Keiko Matsuura, Chisato Nakada, Mizuho Mashio, Takahiro Narimatsu, Taichiro Yoshimoto, Masato Tanigawa, Yoshiyuki Tsukamoto, Naoki Hijiya, Ichiro Takeuchi, Takeo Nomura, Fuminori Sato ...Show all 14 authors... , Hiromitsu Mimata, Masao Seto, Masatsugu Moriyama; Show fewer authors
Downregulation of SAV1 plays a role in pathogenesis of high-grade clear cell renal cell carcinoma.
BMC Cancer 11:523.1-523.10(2011)

PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Jordi Ginesta Barretina, Giordano Caponigro, Nicolas Stransky, Kavitha Venkatesan, Adam A. Margolin, Sungjoon Kim, Christopher J. Wilson, Joseph Lehar, Gregory V. Kryukov, Dmitriy Sonkin, Anupama Reddy ...Show all 61 authors... , Manway Liu, Lauren Murray, Michael F. Berger, John E. Monahan, Paula Morais, Jodi Meltzer, Adam Korejwa, Judit Jane-Valbuena, Felipa A. Mapa, Joseph Thibault, Eva Bric-Furlong, Pichai Raman, Aaron Shipway, Ingo H. Engels, Jill Cheng, Guo-Ying K. Yu, Jian-Jun Yu, Peter Aspesi Jr., Melanie de Silva, Kalpana Jagtap, Michael D. Jones, Li Wang, Charles Hatton, Emanuele Palescandolo, Supriya Gupta, Scott Mahan, Carrie Sougnez, Robert C. Onofrio, Ted Liefeld, Laura E. MacConaill, Wendy Winckler, Michael Reich, Nan-Xin Li, Jill P. Mesirov, Stacey B. Gabriel, Gad Getz, Kristin Ardlie, Vivien Chan, Vic E. Myer, Barbara L. Weber, Jeff Porter, Markus Warmuth, Peter Finan, Jennifer L. Harris, Matthew Langer Meyerson, Todd Robert Golub, Michael P. Morrissey, William Raj Sellers, Robert Schlegel, Levi Alexander Garraway; Show fewer authors
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=22949125; DOI=10.1002/ijc.27822
Rafal Pawlowski, Sarah M. Muhl, Tullio Sulser, Wilhelm Krek, Holger Moch, Peter Schraml;
Loss of PBRM1 expression is associated with renal cell carcinoma progression.
Int. J. Cancer 132:E11-E17(2013)

PubMed=25485619; DOI=10.1038/nbt.3080
Christiaan Klijn, Steffen Durinck, Eric W. Stawiski, Peter M. Haverty, Zhao-Shi Jiang, Han-Bin Liu, Jeremiah Degenhardt, Oleg Mayba, Florian Gnad, Jin-Feng Liu, Gregoire Pau ...Show all 30 authors... , Jens Reeder, Yi Cao, Kiran Mukhyala, Suresh K. Selvaraj, Ma-Mie Yu, Gregory J. Zynda, Matthew J. Brauer, Thomas D. Wu, Robert Clifford Gentleman, Gerard Manning, Robert L. Yauch, Richard Bourgon, David Stokoe, Zora Modrusan, Richard M. Neve, Frederic J. de Sauvage, Jeffrey Settleman, Somasekar Seshagiri, Ze-Min Zhang; Show fewer authors
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Ma-Mie Yu, Suresh K. Selvaraj, May M.Y. Liang-Chu, Sahar Aghajani, Matthew Busse, Jean Yuan, Genee Lee, Franklin V. Peale, Christiaan Klijn, Richard Bourgon, Joshua S. Kaminker, Richard M. Neve;
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5; PMCID=PMC4653878
Jelle Scholtalbers, Sebastian Boegel, Thomas Bukur, Marius Byl, Sebastian Goerges, Patrick Sorn, Martin Loewer, Ugur Sahin, John C. Castle;
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=26972028; DOI=10.1016/j.jprot.2016.03.008
Yusuke Masuishi, Yayoi Kimura, Noriaki Arakawa, Hisashi Hirano;
Identification of glycosylphosphatidylinositol-anchored proteins and omega-sites using TiO2-based affinity purification followed by hydrogen fluoride treatment.
J. Proteomics 139:77-83(2016)

PubMed=27141528; DOI=10.1016/j.dib.2016.04.001; PMCID=PMC4838930
Yusuke Masuishi, Yayoi Kimura, Noriaki Arakawa, Hisashi Hirano;
Data for identification of GPI-anchored peptides and omega-sites in cancer cell lines.
Data Brief 7:1302-1305(2016)

PubMed=27993170; DOI=10.1186/s12943-016-0565-8; PMCID=PMC5168717
Klaudia Klarysa Brodaczewska, Cezary Szczylik, Michal Fiedorowicz, Camillo Porta, Anna Malgorzata Czarnecka;
Choosing the right cell line for renal cell cancer research.
Mol. Cancer 15:83.1-83.15(2016)

PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005; PMCID=PMC5501076
Jun Li, Wei Zhao, Rehan Akbani, Wen-Bin Liu, Zhen-Lin Ju, Shi-Yun Ling, Christopher P. Vellano, Paul Roebuck, Qing-Hua Yu, A. Karina Eterovic, Lauren Averett Byers ...Show all 25 authors... , Michael A. Davies, Wan-Leng Deng, Y.N. Vashisht Gopal, Guo Chen, Erika Maria von Euw, Dennis Joseph Slamon, Dylan Conklin, John Victor Heymach, Adi F. Gazdar, John D. Minna, Jeffrey N. Myers, Yi-Ling Lu, Gordon B. Mills, Han Liang; Show fewer authors
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)

PubMed=28489074; DOI=10.1038/ncomms15165; PMCID=PMC5436135
Rileen Sinha, Andrew G. Winer, Michael Stephen Chevinsky, Christopher Jakubowski, Ying-Bei Chen, Yi-Yu Dong, Satish K. Tickoo, Victor E. Reuter, Paul Russo, Jonathan A. Coleman, Chris Sander ...Show all 13 authors... , James J.-D. Hsieh, Abraham Ari Hakimi; Show fewer authors
Analysis of renal cancer cell lines from two major resources enables genomics-guided cell line selection.
Nat. Commun. 8:15165.1-15165.10(2017)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Julie Dutil, Zhi-Hua Chen, Alvaro N.A. Monteiro, Jamie K. Teer, Steven A. Eschrich;
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Mahmoud Ghandi, Franklin W. Huang, Judit Jane-Valbuena, Gregory V. Kryukov, Christopher C. Lo, E. Robert McDonald 3rd, Jordi Ginesta Barretina, Ellen T. Gelfand, Craig M. Bielski, Hao-Xin Li, Kevin Hu ...Show all 68 authors... , Alexander Y. Andreev-Drakhlin, Jaegil Kim, Julian M. Hess, Brian J. Haas, Francois Aguet, Barbara A. Weir, Michael V. Rothberg, Brenton R. Paolella, Michael Scott Lawrence, Rehan Akbani, Yi-Ling Lu, Hong L. Tiv, Prafulla C. Gokhale, Antoine de Weck, Ali Amin Mansour, Coyin Oh, Juliann Shih, Kevin Hadi, Yanay Rosen, Jonathan Bistline, Kavitha Venkatesan, Anupama Reddy, Dmitriy Sonkin, Manway Liu, Joseph Lehar, Joshua M. Korn, Dale A. Porter, Michael D. Jones, Javad Golji, Giordano Caponigro, Jordan E. Taylor, Caitlin M. Dunning, Amanda L. Creech, Allison C. Warren, James M. McFarland, Mahdi Zamanighomi, Audrey Kauffmann, Nicolas Stransky, Marcin Imielinski, Yosef E. Maruvka, Andrew D. Cherniack, Aviad Tsherniak, Francisca Vazquez, Jacob D. Jaffe, Andrew Alan Lane, David M. Weinstock, Cory M. Johannessen, Michael P. Morrissey, Frank Stegmeier, Robert Schlegel, William Chun Hahn, Gad Getz, Gordon B. Mills, Jesse S. Boehm, Todd Robert Golub, Levi Alexander Garraway, William Raj Sellers; Show fewer authors
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023; PMCID=PMC7339254
David Paul Nusinow, John Szpyt, Mahmoud Ghandi, Christopher M. Rose, E. Robert McDonald 3rd, Marian Kalocsay, Judit Jane-Valbuena, Ellen T. Gelfand, Devin K. Schweppe, Mark P. Jedrychowski, Javad Golji ...Show all 20 authors... , Dale A. Porter, Tomas Rejtar, Y. Karen Wang, Gregory V. Kryukov, Frank Stegmeier, Brian K. Erickson, Levi Alexander Garraway, William Raj Sellers, Steven P. Gygi; Show fewer authors
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)

Cross-references
Cell line collections (Providers) ATCC; HTB-47
BCRJ; 0408
CancerTools; 161904
CLS; 300140
DSMZ; ACC-54
ECACC; 93120819
ICLC; HTL95029
IZSLER; BS TCL 8
KCLB; 30047
Cell line databases/resources CLO; CLO_0002177
MCCL; MCC:0000124
CLDB; cl625
CLDB; cl626
CLDB; cl627
CLDB; cl629
cancercelllines; CVCL_0235
CCRID; 1101HUM-PUMC000337
Cell_Model_Passport; SIDM01546
DepMap; ACH-000234
DSMZCellDive; ACC-54
IGRhCellID; Caki2
Anatomy/cell type resources BTO; BTO_0006204
Biological sample resources 4DN; 4DNSRDD5W2FZ
BioSample; SAMN01821542
BioSample; SAMN01821619
BioSample; SAMN03471873
BioSample; SAMN10988361
ENCODE; ENCBS074UKQ
ENCODE; ENCBS461KDA
ENCODE; ENCBS611WID
ENCODE; ENCBS768KGZ
ENCODE; ENCBS787YGO
CRISP screens repositories BioGRID_ORCS_Cell_line; 583
Chemistry resources ChEMBL-Cells; CHEMBL3308071
ChEMBL-Targets; CHEMBL613852
PharmacoDB; Caki2_164_2019
PubChem_Cell_line; CVCL_0235
Encyclopedic resources Wikidata; Q54808377
Experimental variables resources EFO; EFO_0002150
Gene expression databases ArrayExpress; E-MTAB-38
ArrayExpress; E-MTAB-2706
ArrayExpress; E-MTAB-2770
GEO; GSM374478
GEO; GSM886903
GEO; GSM887968
Polymorphism and mutation databases Cosmic; 687941
Cosmic; 874612
Cosmic; 877458
Cosmic; 979706
Cosmic; 982278
Cosmic; 1152536
Cosmic; 2520633
IARC_TP53; 30022
LiGeA; CCLE_157
Progenetix; CVCL_0235
Proteomic databases PRIDE; PXD000335
PRIDE; PXD003105
Sequence databases EGA; EGAS00001000610
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number41