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Cellosaurus BV-2 (CVCL_0182)

[Text version]
Cell line name BV-2
Synonyms BV2
Accession CVCL_0182
Resource Identification Initiative To cite this cell line use: BV-2 (RRID:CVCL_0182)
Comments Transformant: Recombinant retrovirus J2.
Omics: Proteomics; Subcellular; Secretome.
Omics: Transcriptomics; Microarray.
Omics: Transcriptomics; RNAseq.
Omics: Variations; Array-based CGH.
Derived from site: In situ; Brain; UBERON=UBERON_0000955.
Cell type: Microglial cell; CL=CL_0000129.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: C57BL/6.
Hierarchy Children:
CVCL_ZJ35 (BV-2 PU.1 KO)CVCL_XD67 (BV2-EGFP)CVCL_C8UX (BV2-Luc)
CVCL_WJ28 (BV2-mCherry)CVCL_XD66 (BV2-tDT)CVCL_E1JF (HyCyte BV-2 KO-mAgo2)
CVCL_E1JG (HyCyte BV-2 KO-mCadm1)CVCL_E1JH (HyCyte BV-2 KO-mCd83)CVCL_E1JI (HyCyte BV-2 KO-mNpc1)
CVCL_E1JJ (HyCyte BV-2 KO-mTrem2)CVCL_E3GW (HyCyte BV-2-Luc)CVCL_D8EW (Ubigene BV-2 Ifngr1 KO)
CVCL_D8EX (Ubigene BV-2 Lpl KO)CVCL_D8EY (Ubigene BV-2 Plin2 KO)CVCL_D8EZ (Ubigene BV-2 Syk KO)
CVCL_D8F0 (Ubigene BV-2 Usp7 KO)
Sex of cell Female
Age at sampling 1W
Category Transformed cell line
STR profile Source(s): Ubigene=YC-C035

Markers:
Mouse STR 4-220.3
Mouse STR 5-517
Mouse STR 6-417.3
Mouse STR 6-715
Mouse STR 12-116
Mouse STR 15-322.3,23.3,24.3
Mouse STR 18-316,17
Mouse STR X-127

Run an STR similarity search on this cell line
Publications

PubMed=2110186; DOI=10.1016/0165-5728(90)90073-V
Elisabetta Blasi, Roberta Barluzzi, Virginia Bocchini, Rosanna Mazzolla, Francesco Bistoni;
Immortalization of murine microglial cells by a v-raf/v-myc carrying retrovirus.
J. Neuroimmunol. 27:229-237(1990)

CLPUB00788
Jin Hwan Do, Hyun Myung Ko, Kyoungho Suk, Eun Jung Park, Dong-Kug Choi;
Genome-wide inspection of chromosomal aberrations in microglia BV-2 cells by array-based comparative genomic hybridization.
BioChip J. 3:28-36(2009)

PubMed=19565166; DOI=10.14573/altex.2009.2.83
Anja Henn, Soren Lund, Maj Hedtjarn, Andre Schrattenholz, Peter Porzgen, Marcel Leist;
The suitability of BV2 cells as alternative model system for primary microglia cultures or for animal experiments examining brain inflammation.
ALTEX 26:83-94(2009)

PubMed=21961028; DOI=10.2174/1877381801002010148; PMCID=PMC3180920
Jessica M. Crain, Jyoti J. Watters;
Estrogen and P2 purinergic receptor systems in microglia: therapeutic targets for neuroprotection.
Open Drug Discov. J. 2:148-167(2010)

PubMed=21214938; DOI=10.1186/1471-2164-12-14; PMCID=PMC3024950
Brady K. Atwood, Jacqueline Lopez, James Wager-Miller, Kenneth P. Mackie, Alex Straiker;
Expression of G protein-coupled receptors and related proteins in HEK293, AtT20, BV2, and N18 cell lines as revealed by microarray analysis.
BMC Genomics 12:14.1-14.14(2011)

PubMed=22651808; DOI=10.1186/1742-2094-9-115; PMCID=PMC3407712
Branden Stansley, Jan Post, Kenneth Hensley;
A comparative review of cell culture systems for the study of microglial biology in Alzheimer's disease.
J. Neuroinflammation 9:115.1-115.8(2012)

PubMed=26227174; DOI=10.1002/pmic.201400623
Jong-Min Woo, Do-Hyun Han, Joonho Park, Sang Jeong Kim, Youngsoo Kim;
In-depth characterization of the secretome of mouse CNS cell lines by LC-MS/MS without prefractionation.
Proteomics 15:3617-3622(2015)

DOI=10.1007/s13273-019-00057-6
Chanchal Mandal, Taeho Yoon, Ji Yoon Park, Kyoung Hwa Jung, Young Gyu Chai;
Lipopolysaccharide-dependent transcriptional regulation of PU.1 in microglial cells.
Mol. Cell. Toxicol. 16:51-61(2020)

Cross-references
Cell line collections (Providers) BCRJ; 0356
CCTCC; GDC0311
CLS; 305156
ICLC; ATL03001
KCB; KCB 200770YJ
Ubigene; YC-C035
Cell line databases/resources MCCL; MCC:0000073
CLDB; cl7130
CCRID; 1101MOU-PUMC000063
CCRID; 4201MOU-CCTCC00311
Lonza; 118
Anatomy/cell type resources BTO; BTO_0003350
CRISP screens repositories BioGRID_ORCS_Cell_line; 765
Chemistry resources ChEMBL-Cells; CHEMBL3307704
ChEMBL-Targets; CHEMBL614781
PubChem_Cell_line; CVCL_0182
Encyclopedic resources Wikidata; Q54798657
Experimental variables resources EFO; EFO_0022792
Gene expression databases GEO; GSM635468
GEO; GSM635469
GEO; GSM635470
GEO; GSM635471
Proteomic databases PRIDE; PXD001597
PRIDE; PXD004984
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number29