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Cellosaurus 3T3-L1 (CVCL_0123)

[Text version]
Cell line name 3T3-L1
Synonyms 3T3 L1; 3T3L1; 3T3-L1 ad; NIH-3T3-L1; NIH3T3-L1
Accession CVCL_0123
Resource Identification Initiative To cite this cell line use: 3T3-L1 (RRID:CVCL_0123)
Comments Doubling time: ~2.2 days (lot 12142004), 31 hours (lot 07032008), ~28 hours (lot 07232012), ~54 hours (lot 02202015), ~39 hours (lot 08012016), ~28 hours (lot 02212018) (JCRB).
Miscellaneous: STR profile from personal communication of Gemma R.-T.
Derived from sampling site: Whole embryo. Cell type=Fibroblast.
Species of origin Mus musculus (Mouse) (NCBI Taxonomy: 10090)
Breed/subspecies: Swiss albino.
Hierarchy Parent: CVCL_0120 (3T3-Swiss albino)
Children:
CVCL_QZ17 (3T3-L1 EGFP-ATA2)CVCL_0A20 (3T3-L1 MBX)CVCL_0122 (3T3F442A)
Sex of cell Male
Age at sampling Embryo
Category Spontaneously immortalized cell line
STR profile Source(s): Direct_author_submission

Markers:
Mouse STR 1-110,15
Mouse STR 1-213
Mouse STR 2-19
Mouse STR 3-214
Mouse STR 4-219.3
Mouse STR 5-513,15
Mouse STR 6-415.3
Mouse STR 6-712,15
Mouse STR 7-125.2,29
Mouse STR 8-115,16
Mouse STR 11-215
Mouse STR 12-119
Mouse STR 13-115
Mouse STR 15-320.3
Mouse STR 17-212,15
Mouse STR 18-317,21
Mouse STR 19-212
Mouse STR X-126

Run an STR similarity search on this cell line
Web pages https://en.wikipedia.org/wiki/3T3-L1
http://www.cells-talk.com/index.php/page/copelibrary?key=3T3-L1%20cells
http://www.zen-bio.com/products/cells/3T3L1.php
https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/n/cell-lines-detail-72.html
https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/n/cell-lines-detail-103.html
https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/n/cell-lines-detail-334.html
Publications

DOI=10.1016/0092-8674(74)90126-3
Green H., Kehinde O.
Sublines of mouse 3T3 cells that accumulate lipid.
Cell 1:113-116(1974)

PubMed=4426090; DOI=10.1016/0092-8674(74)90116-0
Green H., Meuth M.
An established pre-adipose cell line and its differentiation in culture.
Cell 3:127-133(1974)

PubMed=165899; DOI=10.1016/0092-8674(75)90087-2
Green H., Kehinde O.
An established preadipose cell line and its differentiation in culture. II. Factors affecting the adipose conversion.
Cell 5:19-27(1975)

Patent=US4003789
Green H.
Triglyceride-accumulating clonal cell line.
Patent number US4003789, 18-Jan-1977

PubMed=6698114; DOI=10.1016/0014-4827(84)90361-6
Levine J.F., Stockdale F.E.
3T3-L1 adipocytes promote the growth of mammary epithelium.
Exp. Cell Res. 151:112-122(1984)

PubMed=28176856; DOI=10.1038/srep42104
Kassotis C.D., Masse L., Kim S., Schlezinger J.J., Webster T.F., Stapleton H.M.
Characterization of adipogenic chemicals in three different cell culture systems: implications for reproducibility based on cell source and handling.
Sci. Rep. 7:42104-42104(2017)

Cross-references
Cell line collections AddexBio; P0011002/407
ATCC; CL-173
ATCC; CCL-92.1 - Discontinued
BCRC; 60159
BCRJ; 0019
CCTCC; GDC0180
CLS; 400103
ECACC; 86052701
IBRC; C10152
IZSLER; BS CL 100
JCRB; IFO50416
JCRB; JCRB9014
KCB; KCB 92010YJ
KCLB; 10092.1
Kerafast; EF3001
Cell line databases/resources CLO; CLO_0001352
MCCL; MCC:0000015
CLDB; cl71
CLDB; cl72
CLDB; cl73
CCRID; 1101MOU-PUMC000155
CCRID; 3101MOUGNM25
CCRID; 3101MOUSCSP5038
CCRID; 4201MOU-CCTCC00180
CCRID; 5301MOU-KCB92010YJ
Lonza; 677
Lonza; 1083
TOKU-E; 331
Anatomy/cell type resources BTO; BTO:0000011
Biological sample resources ENCODE; ENCBS366WMO
ENCODE; ENCBS666CGP
Chemistry resources ChEMBL-Cells; CHEMBL3307630
ChEMBL-Targets; CHEMBL614510
PubChem_Cell_line; CVCL_0123
Encyclopedic resources Wikidata; Q4636414
Experimental variables resources EFO; EFO_0001084
EFO; EFO_0001223
Medical resources MeSH; D041721
Proteomic databases PRIDE; PXD001063
PRIDE; PXD001529
PRIDE; PXD003696
Entry history
Entry creation04-Apr-2012
Last entry update21-Mar-2023
Version number30