ID   3T3-L1
AC   CVCL_0123
SY   3T3 L1; 3T3L1; 3T3-L1 ad; NIH-3T3-L1; NIH3T3-L1
DR   BTO; BTO_0000011
DR   CLO; CLO_0001352
DR   EFO; EFO_0001084
DR   MCCL; MCC:0000015
DR   CLDB; cl71
DR   CLDB; cl72
DR   CLDB; cl73
DR   AddexBio; P0011002/407
DR   ATCC; CCL-92.1
DR   ATCC; CL-173
DR   BCRC; 60159
DR   BCRJ; 0019
DR   CCRID; 1101MOU-PUMC000155
DR   CCRID; 3101MOUGNM25
DR   CCRID; 3101MOUSCSP5038
DR   CCRID; 4201MOU-CCTCC00180
DR   CCRID; 5301MOU-KCB92010YJ
DR   CCTCC; GDC0180
DR   ChEMBL-Cells; CHEMBL3307630
DR   ChEMBL-Targets; CHEMBL614510
DR   CLS; 400107
DR   ECACC; 86052701
DR   ENCODE; ENCBS366WMO
DR   ENCODE; ENCBS666CGP
DR   IBRC; C10152
DR   IZSLER; BS CL 100
DR   JCRB; IFO50416
DR   JCRB; JCRB9014
DR   KCB; KCB 92010YJ
DR   KCLB; 10092.1
DR   Kerafast; EF3001
DR   Lonza; 677
DR   Lonza; 1083
DR   MeSH; D041721
DR   PRIDE; PXD001063
DR   PRIDE; PXD001529
DR   PRIDE; PXD003696
DR   PubChem_Cell_line; CVCL_0123
DR   TOKU-E; 331
DR   Wikidata; Q4636414
RX   DOI=10.1016/0092-8674(74)90126-3;
RX   Patent=US4003789;
RX   PubMed=165899;
RX   PubMed=4426090;
RX   PubMed=6698114;
RX   PubMed=28176856;
WW   Info; COPE; -; http://www.cells-talk.com/index.php/page/copelibrary?key=3T3-L1%20cells
WW   Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/n/cell-lines-detail-103.html
WW   Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/n/cell-lines-detail-334.html
WW   Info; Thermo Fisher; -; https://www.thermofisher.com/ch/en/home/technical-resources/cell-lines/n/cell-lines-detail-72.html
WW   Info; Wikipedia; -; https://en.wikipedia.org/wiki/3T3-L1
WW   Provider; BRICS; BRICS0073; http://www.brics.ac.cn/cell/template/cell/cell_detail.html?id=3888
WW   Provider; ZenBio; -; https://www.zen-bio.com/products/cells/3T3L1.php
CC   Doubling time: ~2.2 days (Note=Lot 12142004), 31 hours (Note=Lot 07032008), ~28 hours (Note=Lot 07232012), ~54 hours (Note=Lot 02202015), ~39 hours (Note=Lot 08012016), ~28 hours (Note=Lot 02212018) (JCRB=IFO50416); ~43 hours (Note=Lot 11272012), ~41 hours (Note=Lot 02132012), ~28 hours (Note=Lot 08222011), ~23 hours (Note=Lot 02152011), ~26 hours (Note=Lot 01142010), ~31 hours (Note=Lots 01282009 and 08222016), ~1 day (Note=Lot 01252008), ~1.5 days (Note=Lot 02092007), ~33 hours (Note=Lots 06122006 and 05252015), ~40 hours (Note=Lot 12162004), 60-70 hours (Note=Lot 06082004), ~3 days (Note=Lot 10302002), ~37 hours (Note=Lot 032499), ~30 hours (Note=Lot 06012015), 20-64 hours (Note=Lot 04212017), ~27 hours (Note=Lot 10082020) (JCRB=JCRB9014).
CC   Miscellaneous: STR profile from personal communication of Rodriguez-Tarduchy Segovia, Gemma.
CC   Discontinued: ATCC; CCL-92.1; true.
CC   Derived from site: In situ; Whole embryo; UBERON=UBERON_0000922.
CC   Cell type: Fibroblast; CL=CL_0000057.
CC   Breed/subspecies: Swiss albino.
ST   Source(s): Direct_author_submission
ST   Mouse STR 1-1: 10,15
ST   Mouse STR 1-2: 13
ST   Mouse STR 2-1: 9
ST   Mouse STR 3-2: 14
ST   Mouse STR 4-2: 19.3
ST   Mouse STR 5-5: 13,15
ST   Mouse STR 6-4: 15.3
ST   Mouse STR 6-7: 12,15
ST   Mouse STR 7-1: 25.2,29
ST   Mouse STR 8-1: 15,16
ST   Mouse STR 11-2: 15
ST   Mouse STR 12-1: 19
ST   Mouse STR 13-1: 15
ST   Mouse STR 15-3: 20.3
ST   Mouse STR 17-2: 12,15
ST   Mouse STR 18-3: 17,21
ST   Mouse STR 19-2: 12
ST   Mouse STR X-1: 26
OX   NCBI_TaxID=10090; ! Mus musculus (Mouse)
HI   CVCL_0120 ! 3T3-Swiss albino
SX   Male
AG   Embryo
CA   Spontaneously immortalized cell line
DT   Created: 04-04-12; Last updated: 10-04-25; Version: 35
CH   CVCL_D4DA ! 3T3-L1 CAR SNRK KO
CH   CVCL_QZ17 ! 3T3-L1 EGFP-ATA2
CH   CVCL_0A20 ! 3T3-L1 MBX
CH   CVCL_E6YT ! 3T3-L1-GLUT4myc
CH   CVCL_0122 ! 3T3F442A
CH   CVCL_D6A9 ! HyCyte 3T3-L1 KO-mAckr3
CH   CVCL_E1IK ! HyCyte 3T3-L1 KO-mFasn
CH   CVCL_F1LI ! HyCyte 3T3-L1 KO-mSfrp5
//
RX   PubMed=4426090; DOI=10.1016/0092-8674(74)90116-0;
RA   Green, Howard
RA   Meuth, Mark
RT   "An established pre-adipose cell line and its differentiation in
RT   culture.";
RL   Cell 3:127-133(1974).
//
RX   PubMed=165899; DOI=10.1016/0092-8674(75)90087-2;
RA   Green, Howard
RA   Kehinde, Olaniyi
RT   "An established preadipose cell line and its differentiation in
RT   culture. II. Factors affecting the adipose conversion.";
RL   Cell 5:19-27(1975).
//
RX   PubMed=28176856; DOI=10.1038/srep42104; PMCID=PMC5296734;
RA   Kassotis, Christopher D.
RA   Masse, Lauren
RA   Kim, Stephanie
RA   Schlezinger, Jennifer Joy
RA   Webster, Thomas F.
RA   Stapleton, Heather M.
RT   "Characterization of adipogenic chemicals in three different cell
RT   culture systems: implications for reproducibility based on cell source
RT   and handling.";
RL   Sci. Rep. 7:42104.1-42104.17(2017).
//
RX   Patent=US4003789;
RA   Green, Howard
RT   "Triglyceride-accumulating clonal cell line.";
RL   Patent number US4003789, 18-Jan-1977.
//
RX   PubMed=6698114; DOI=10.1016/0014-4827(84)90361-6;
RA   Levine, Jedd F.
RA   Stockdale, Frank E.
RT   "3T3-L1 adipocytes promote the growth of mammary epithelium.";
RL   Exp. Cell Res. 151:112-122(1984).
//
RX   DOI=10.1016/0092-8674(74)90126-3;
RA   Green, Howard
RA   Kehinde, Olaniyi
RT   "Sublines of mouse 3T3 cells that accumulate lipid.";
RL   Cell 1:113-116(1974).
//