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Cellosaurus 16HBE14o- (CVCL_0112)

[Text version]
Cell line name 16HBE14o-
Synonyms 16-HBE14o; 16HBE140; 16-HBEo; 16HBEo-; 16-HBE; 16HBE
Accession CVCL_0112
Resource Identification Initiative To cite this cell line use: 16HBE14o- (RRID:CVCL_0112)
Comments Characteristics: Widely used to model barrier function of the airway epithelium and to study respiratory ion transport as well as the function of CFTR (Millipore=SCC150).
Transformant: NCBI_TaxID; 1891767; Simian virus 40 (SV40) (Note=pSVori-).
Omics: Proteomics; 2DE-MS.
Omics: Variations; Array-based CGH.
Derived from site: In situ; Lung, bronchus, epithelium; UBERON=UBERON_0002031.
Cell type: Epithelial cell of bronchus; CL=CL_0002328.
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Children:
CVCL_ZW91 (HBE-1A1)CVCL_S124 (NHBE-T)
Sex of cell Male
Age at sampling 1Y
Category Transformed cell line
STR profile Source(s): Millipore=SCC150

Markers:
AmelogeninX
CSF1PO11
D3S135816
D5S81811,12
D7S82011
D8S117910,11
D13S31711
D16S53912,13
D18S5114
D21S1128,30.2
FGA21,22
Penta D9
Penta E5,14
TH019.3
TPOX8
vWA16,18

Run an STR similarity search on this cell line
Web pages Info; Dartmouth; -; https://web.archive.org/web/20190126194809/http://www.dartmouth.edu/~cysfib/cell-lines.html
Publications

PubMed=1282304; DOI=10.1152/ajplung.1992.263.6.L692
Christine Haws, Mauri E. Krouse, Yi-Fan Xia, Dieter C. Gruenert, Jeffrey J. Wine;
CFTR channels in immortalized human airway cells.
Am. J. Physiol. 263:L692-L707(1992)

PubMed=7507342; DOI=10.1165/ajrcmb.10.1.7507342
Alison L. Cozens, Michael J. Yezzi, Karl Kunzelmann, Takashi Ohrui, Lynda Chin, Ken Eng, Walter E. Finkbeiner, Jonathan H. Widdicombe, Dieter C. Gruenert;
CFTR expression and chloride secretion in polarized immortal human bronchial epithelial cells.
Am. J. Respir. Cell Mol. Biol. 10:38-47(1994)

PubMed=9614386; DOI=10.1002/(SICI)1096-9896(199803)184:3<323::AID-PATH2>3.0.CO;2-2
Qing-Hai Meng, David R. Springall, Anne E. Bishop, Kevin Morgan, Tom J. Evans, Said Habib, Dieter C. Gruenert, Khin M. Gyi, Margaret E. Hodson, Magdi Habib Yacoub, Julia Margaret Polak;
Lack of inducible nitric oxide synthase in bronchial epithelium: a possible mechanism of susceptibility to infection in cystic fibrosis.
J. Pathol. 184:323-331(1998)

PubMed=10498856; DOI=10.1038/sj.bjp.0702772; PMCID=PMC1571611
Kaarin K. Goncz, Luzviminda Feeney, Dieter C. Gruenert;
Differential sensitivity of normal and cystic fibrosis airway epithelial cells to epinephrine.
Br. J. Pharmacol. 128:227-233(1999)

PubMed=12711171; DOI=10.1016/S0378-5173(03)00129-7
Ben Forbes, Atiya Shah, Gary P. Martin, Alison B. Lansley;
The human bronchial epithelial cell line 16HBE14o- as a model system of the airways for studying drug transport.
Int. J. Pharm. 257:161-167(2003)

PubMed=15463957; DOI=10.1016/j.jcf.2004.05.040
Dieter C. Gruenert, Marjolaine Willems, Jean-Jacques Cassiman, Raymond A. Frizzell;
Established cell lines used in cystic fibrosis research.
J. Cyst. Fibros. 3:191-196(2004)

PubMed=18441018; DOI=10.1194/jlr.M700388-JLR200; PMCID=PMC2444007
Charlotte Andersson, M. Rabie Al-Turkmani, Juanito E. Savaille, Ragheed Alturkmani, Waddah Katrangi, Joanne E. Cluette-Brown, Munir M. Zaman, Michael Laposata, Steven D. Freedman;
Cell culture models demonstrate that CFTR dysfunction leads to defective fatty acid composition and metabolism.
J. Lipid Res. 49:1692-1700(2008)

PubMed=28970102; DOI=10.1016/j.jprot.2017.09.013
Michele Puglia, Claudia Landi, Assunta Gagliardi, Loretta Breslin, Alessandro Armini, Jlenia Brunetti, Alessandro Pini, Laura Bianchi, Luca Bini;
The proteome speciation of an immortalized cystic fibrosis cell line: new perspectives on the pathophysiology of the disease.
J. Proteomics 170:28-42(2018)

Cross-references
Cell line collections (Providers) CLS; 305234
Millipore; SCC150
Cell line databases/resources MCCL; MCC:0000003
TOKU-E; 4023
Anatomy/cell type resources BTO; BTO_0002734
Chemistry resources ChEMBL-Cells; CHEMBL4295404
ChEMBL-Targets; CHEMBL4296395
PubChem_Cell_line; CVCL_0112
Encyclopedic resources Wikidata; Q54582314
Gene expression databases ArrayExpress; E-MTAB-4194
Proteomic databases PRIDE; PXD006933
Entry history
Entry creation04-Apr-2012
Last entry update10-Apr-2025
Version number22