| Publication number |
CLPUB00808 |
|---|
| Authors |
H. Karasawa, Masahide Hasobe, Hideo Fukuda, Tokuo Sano; |
|---|
| Title |
Development of a suspension culture of chinook salmon (Oncorhynchus tshawytscha) embryo (CHSE-214) cells in a spinner flask. |
|---|
| Citation |
Bull. Eur. Assoc. Fish Pathol. 11:142-144(1991) |
|---|
| Web pages |
https://eafp.org/download/1991-Volume11/Issue%204/4-Karasawa%20et%20al.pdf |
|---|
| Abstract |
Cultivation of fish cells is generally carried out under static conditions
using a flask or a dish providing the surface for cell adhesion. However,
a notable report by Lidgerding (1981) described the development of
suspension culture of fish cells such as chinook salmon (Oncorhynchus
tshawytscha) embryo (CHSE-214) cells and fathead minnow (Pimephales
promelas) (FHM) cells, in a viscid culture medium using 0.15%
carboxymethylcellulose (CMC). However, the potentiality of such cultures
to support the replication of some viruses was poor.
In the present study we conducted the suspension culture of CHSE-214 cells
in a spinner flask with normal culture medium in order to retain the
susceptibility to viruses.
|
|---|
| Cell lines |
CVCL_L017; CHSE-sp |
|---|
