Publication number |
CLPUB00801 |
Authors |
Zhang Y.-M., Li X., Xu X.-M., Luo W.-J. |
Title |
Construction of a high-EGFR expression cell line and its biological properties comparing with A431 cell. |
Citation |
Afr. J. Biotechnol. 9:4674-4680(2010) |
Web pages |
https://www.ajol.info/index.php/ajb/article/view/82985https://www.researchgate.net/publication/267793292 |
Abstract |
Targeted screening of EGFR compounds has become one of the medical
research focuses for tumor therapy. A431, which naturally expresses high
levels of EGFR, was compared with the stably high expressing EGFR cell
line HEK293. Flow cytometry was used to analyze cell growth and Western
blot was used to determine expression of EGFR, p-EGFR and p-ERK. Cellular
membrane chromatography analysis revealed longer on-peak chromatographic
and greater on-peak area of A431. Results demonstrated that the high-
expression EGFR cell line A431 improved sensitivity of cellular membrane
chromatography when screening drugs targeted to EGFR.
|
Cell lines |
CVCL_E8AY; HEK293-EGFR |